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Cell Structure and Transport 7-1 Objectives • Describe the tenets of the cell theory • Compare the characteristics of prokaryotic and eukaryotic cells • Describe the parts of a light and electron microscope Chapter 7 Cellular Structure and Function 7.1 Cell Discovery and Theory The Cell Theory All organisms are composed of one or more cells. The cell is the basic unit of structure and organization of organisms. All cells come from preexisting cells. Chapter 7 Cellular Structure and Function 7.1 Cell Discovery and Theory Basic Cell Types - All cells have a plasma membrane Prokaryotic Cell Simple structure Contains a plasma membrane Does not contain membrane-bound organelles No nucleus 11,000x Chapter 7 Cellular Structure and Function 7.1 Cell Discovery and Theory Eukaryotic Cell More complex structure Contains a plasma membrane Membrane cell organelles 7.2 The Plasma Membrane Objectives 1. Describe how a cell’s plasma membrane functions 2. Identify the roles of proteins, carbohydrates, and cholesterol in the plasma membrane Chapter 7 Cellular Structure and Function 7.2 The Plasma Membrane Plasma Membrane Thin, flexible boundary between the cell and its environment Chapter 7 Cellular Structure and Function 7.2 The Plasma Membrane Selective Permeability The plasma membrane controls the movement of substances into and out of the cell. Chapter 7 Cellular Structure and Function 7.2 The Plasma Membrane The plasma membrane is composed of the phospholipid bilayer. Chapter 7 Cellular Structure and Function 7.2 The Plasma Membrane Fluid Mosaic Model “float” in the membrane. Other Components Proteins Cholesterol Carbohydrates Chapter 7 Cellular Structure and Function 7.2 The Plasma Membrane Proteins Transmit signals inside the cell support structure Provide pathways for substances to enter and leave Chapter 7 Cellular Structure and Function 7.2 The Plasma Membrane Cholesterol Prevents fatty acid tails from sticking together Chapter 7 Cellular Structure and Function 7.2 The Plasma Membrane Carbohydrates Identify chemical signals • Plasma membrane 7.3 Structures and Organelles Objectives 1. Identify the structure and function of the parts of a typical eukaryotic cell. 2. Compare and contrast structures of plant and animal cells. Chapter 7 Cellular Structure and Function 7.3 Structures and Organelles Plant and Animal Cell Structures Animal Cell Plant Cell Chapter 7 Cellular Structure and Function 7.3 Structures and Organelles Chapter 7 Cellular Structure and Function 7.3 Structures and Organelles Chapter 7 Cellular Structure and Function 7.3 Structures and Organelles Chapter 7 Cellular Structure and Function 7.3 Structures and Organelles Chapter 7 Cellular Structure and Function 7.3 Structures and Organelles Cilia Short, numerous projections that look like hairs 400x Flagella Longer and less numerous than cilia Create movement with a whiplike motion 26,367x Summary of cell parts • http://glencoe.mcgrawhill.com/olcweb/cgi/pluginpop.cgi?it=swf::550::400::/sites/dl/free/0 078695104/383931/Table7_1a.swf::Summary%20of%20Cell%20Str uctures%20-%20A • http://glencoe.mcgrawhill.com/olcweb/cgi/pluginpop.cgi?it=swf::550::400::/sites/dl/free/0 078695104/383931/Table7_1b.swf::Summary%20of%20Cell%20Str uctures%20-%20B • http://glencoe.mcgrawhill.com/olcweb/cgi/pluginpop.cgi?it=swf::550::400::/sites/dl/free/0 078695104/383931/Table7_1c.swf::Summary%20of%20Cell%20Str uctures%20-%20C 7.4 Cellular Transport Objectives 1. Explain the processes of diffusion, facilitated diffusion, and active transport 2. Predict the effect of a hypotonic, hypertonic, or isotonic solution on a cell 3. Discuss how large particles enter and exit cells Cell Membranes & Movement Across Them 2006-2007 Cell (plasma) membrane • Cells need an inside & an outside… – separate cell from its environment – cell membrane is the boundary IN food - sugars - proteins - fats salts O2 H2O cell needs materials in & products or waste out OUT waste - ammonia - salts - CO2 - H2O products - proteins Building a membrane • How do you build a barrier that keeps the watery contents of the cell separate from the watery environment? FATS LIPIDS Remember: oil & water don’t mix!! What substance do you know that doesn’t mix with water? Lipids of cell membrane • Membrane is made of special kind of lipid – phospholipids – “split personality” “attracted to water” • Membrane is a double layer – phospholipid bilayer phosphate inside cell lipid outside cell “repelled by water” Semi-permeable membrane • Cell membrane controls what gets in or out • Need to allow some materials — but not all — to pass through the membrane – semi-permeable • only some material can get in or out So what needs to get across the membrane? sugar lipids aa O2 H2O salt waste Crossing the cell membrane • What molecules can get through the cell membrane directly? – fats and oils can pass directly through lipid inside cell salt waste outside cell sugar aa H2O but… what about other stuff? Cell membrane channels • Need to make “doors” through membrane – protein channels allow substances in & out • specific channels allow specific material in & out • H2O channel, salt channel, sugar channel, etc. inside cell waste H2O salt aa sugar outside cell How do you build a semi-permeable cell membrane? • Channels are made of proteins – proteins both “like” water & “like” lipids bi-lipid membrane protein channels in bi-lipid membrane Protein channels • Proteins act as doors in the membrane – channels to move specific molecules through cell membrane HIGH LOW Movement through the channel • Why do molecules move through membrane if you give them a channel? HIGH ? LOW ? Molecules move from high to low • Diffusion – move from HIGH to LOW concentration Diffusion • Move from HIGH to LOW concentration – passive transport – no energy needed diffusion diffusion of water osmosis Simple Diffusion • Move from HIGH to LOW fat inside cell fat fat fat fat fat Which way will fat move? LOW HIGH fat outside cell fat fat fat fat fat fat fat Facilitated Diffusion • Move from HIGH to LOW through a channel sugar inside cell sugar sugar sugar sugar sugar LOW Which way will sugar move? HIGH outside cell sugar sugar sugar sugar sugar sugar sugar Diffusion • Move from HIGH to LOW concentration – directly through membrane • simple diffusion • no energy needed – help through a protein channel • facilitated diffusion (with help) • no energy needed HIGH LOW Simple vs. facilitated diffusion simple diffusion inside cell lipid facilitated diffusion inside cell H2O protein channel outside cell outside cell H2O Active transport • Cells may need molecules to move against concentration “hill” – need to pump “uphill” • from LOW to HIGH using energy – protein pump – requires energy • ATP ATP Transport summary simple diffusion facilitated diffusion active transport ATP Osmosis Movement of Water Across Cell Membrane 2006-2007 Osmosis • Water is very important, so we talk about water separately • Osmosis – diffusion of water from HIGH concentration of water to LOW concentration of water • across a semi-permeable membrane Keeping water balance • Cell survival depends on balancing water uptake & water loss freshwater balanced saltwater 1 • Keeping right amount of water in cell Freshwater freshwater KABOOM! – a cell in fresh water – high concentration of water around cell • cell gains water • example: Paramecium • problem: cells gain water, swell & can burst – water continually enters Paramecium cell • solution: contractile vacuole – pumps water out of cell No problem, here Controlling water • Contractile vacuole in Paramecium 2 Keeping right amount of water in cell I’m shrinking, saltwater • Saltwater I’m shrinking! – a cell in salt water – low concentration of water around cell • cell loses water – example: shellfish – problem: cell loses water • in plants: plasmolysis • in animals: shrinking cell – solution: take up water I will survive! 3 Keeping right amount of water in • Balanced conditionscell That’s balanced better! – no difference in concentration of water between cell & environment • cell in equilibrium • example: blood • problem: none I could – water flows across membrane be better… equally, in both directions – volume of cell doesn’t change Ice Fishing in Barrow QuickTime™ and a YUV420 codec decompressor are needed to see this picture.