Download EPC (Skin, Fish)

yes no Was this document useful for you?
   Thank you for your participation!

* Your assessment is very important for improving the work of artificial intelligence, which forms the content of this project

Document related concepts

Induced pluripotent stem cell wikipedia, lookup

Epigenetics in stem-cell differentiation wikipedia, lookup

Embryonic stem cell wikipedia, lookup

Hematopoietic stem cell wikipedia, lookup

Artificial cell wikipedia, lookup

Ehud Shapiro wikipedia, lookup

Somatic cell nuclear transfer wikipedia, lookup

Tissue engineering wikipedia, lookup

Cell encapsulation wikipedia, lookup

Polyclonal B cell response wikipedia, lookup

Cellular differentiation wikipedia, lookup

Vectors in gene therapy wikipedia, lookup

Bio-MEMS wikipedia, lookup

Cat. No. EC93120820
EPC (Skin, Fish)
Culture Medium:
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Fetal
Bovine Serum (FBS).
Cell Line description:
This cell line known as Epithelioma papulosum cyprini (EPC) was originally reported to be
from Carp (Cyprinus carpio) epidermal herpes virus-induced hyperplastic lesions. More
recently EPC was found to be derived from Fathead Minnow (Pimephales promelas) see
Winton et al., 2010. This has been confirmed by DNA sequencing at ECACC. The cells support
the growth of pancreatic necrosis virus and rhabdovirus.
Growth Mode:Adherent
Sub Culture Routine:Split sub-confluent cultures (70-80%) 1:3 using 0.25% trypsin/EDTA; 5%
CO2; 20°C. Fish cell lines detach easily during transit if the culture is too young. After
resuscitation split cells 1:3 and culture for at least one week with 1-2 media changes before
Karyotype:2n = 96
Depositor:Dr M Ferrari, Instituto Zooprofilattico, Brescia
References:Fijan N et al., 1983 Some properties of the epithelioma papulosum cyprini (EPC)
cell line from Carp Cyprinus carpio. Ann. Virol. (Inst. Pasteur) 134E 207-220.
Winton J, Batts W, deKinkelin P, LeBerre M, Bremont M, Fijan N 2010 Current lineages of
the epithelioma papulosum cyprini (EPC) cell line are contaminated with fathead minnow,
Pimephales promelas, cells. J Fish Dis. 33(8):701-4 PMID: 20497291.