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Transcript
Cat. No. EC93120820
EPC (Skin, Fish)
Culture Medium:
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Fetal
Bovine Serum (FBS).
Cell Line description:
This cell line known as Epithelioma papulosum cyprini (EPC) was originally reported to be
from Carp (Cyprinus carpio) epidermal herpes virus-induced hyperplastic lesions. More
recently EPC was found to be derived from Fathead Minnow (Pimephales promelas) see
Winton et al., 2010. This has been confirmed by DNA sequencing at ECACC. The cells support
the growth of pancreatic necrosis virus and rhabdovirus.
DESCRIPTION OF REPOSITORY PEFERENCE SEED STOCK
Morphology:Epithelial
Growth Mode:Adherent
Sub Culture Routine:Split sub-confluent cultures (70-80%) 1:3 using 0.25% trypsin/EDTA; 5%
CO2; 20°C. Fish cell lines detach easily during transit if the culture is too young. After
resuscitation split cells 1:3 and culture for at least one week with 1-2 media changes before
shipping.
Karyotype:2n = 96
Depositor:Dr M Ferrari, Instituto Zooprofilattico, Brescia
Country:ITALY
References:Fijan N et al., 1983 Some properties of the epithelioma papulosum cyprini (EPC)
cell line from Carp Cyprinus carpio. Ann. Virol. (Inst. Pasteur) 134E 207-220.
Winton J, Batts W, deKinkelin P, LeBerre M, Bremont M, Fijan N 2010 Current lineages of
the epithelioma papulosum cyprini (EPC) cell line are contaminated with fathead minnow,
Pimephales promelas, cells. J Fish Dis. 33(8):701-4 PMID: 20497291.