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Leading Developer and Manufacturer of In Vivo and DNA Transfection
Kits, Transfection Reagents and Electroporation Delivery Products
Products > CHO Transfection Reagent (Chinese Hamster Ovary Cells)
Altogen Biosystems offers the CHO Cell Transfection Reagent
among a host of 100+ cell line specific In Vitro Transfection Kits.
The CHO
Cell Transfection Reagent is a lipid mediated
formulation, and it has been developed to provide high
transfection efficiency with CHO.
This cell line is a good host for studying a range of areas within
the field of biomedical therapeutics and biology. When cultured in
vitro, CHO cells are a monolayer and adhere to the surface of the
culture flask.
Other applications include drug discovery, gene expression
studies, molecular and cell biology research applications.
Purchase CHO Transfection Kit at www.Altogen.com
Altogen Biosystems  848 Rainbow Blvd #823  Las Vegas  NV  89107  USA
Telephone  702 349 6103  Fax  702 989-0841  email  [email protected]
Leading Developer and Manufacturer of In Vivo and DNA Transfection
Kits, Transfection Reagents and Electroporation Delivery Products
Products > CHO Transfection Reagent (Chinese Hamster Ovary Cells)
CHO Cell Line Description and Applications
CHO cells
www.microscopyu.com
The CHO or Chinese Hamster Ovary cell line was
established using ovaran cells from a Chinese Hamster
(Cricetulus griseus) in 1957. Chinese Hamster Ovary
cells display epithelial morphology. This cell line has a
far reach as it is widely used in transfection and the
production of therapeutic proteins and recombinant
proteins. Beyond commercial applications, the CHO cell
line has application for biological and biomedical
research. For example, the cell line can be used in
studies ranging from toxicity screening to gene
expression and nutrition.
Altogen Biosystems  848 Rainbow Blvd #823  Las Vegas  NV  89107  USA
Telephone  702 349 6103  Fax  702 989-0841  email  [email protected]
Leading Developer and Manufacturer of In Vivo and DNA Transfection
Kits, Transfection Reagents and Electroporation Delivery Products
Products > CHO Transfection Reagent (Chinese Hamster Ovary Cells)
CHO Transfection Protocol
1. Plate 10,000 - 15,000 CHO cells per well in 0.5 ml of complete growth medium 12–24 hours prior to
transfection
2. Wash with 1xPBS and add 0.5 ml of fresh growth medium
3. Prepare transfection complexes by mixing 40 µl of serum-free medium, 5.5 µl of transfection reagent,
and
• 750 ng DNA (or mRNA), or
• 30 nM - 50 nM of siRNA (or microRNA)
*Referred to a final volume including growth medium
4. Incubate transfection complexes at RT for 15 - 30 minutes
5. Optional: Add 2 µl of Complex Condenser. This reagent reduces the size of transfection complex,
therefore increasing transfection efficiency; however it may increase cell toxicity
6. Add prepared transfection complexes to 0.5 ml of complete growth medium with CHO cells (from step
2)
7. Incubate cells at 37ºC in a humidified CO2 incubator
8. Assay for phenotype or target gene expression 48 - 72 hours after transfection
Optional: Transfection efficiency can be increased by addition of Transfection Enhancer reagent. Add 2 µl of
Transfection Enhancer reagent 12-24 hours after transfection
If the viability of CHO cells being transfected is affected at 16 - 24 hours post-transfection, the level of cytotoxicity
can be decreased by changing the growth medium and eliminating redundant exposure of cells to transfectant
Altogen Biosystems  848 Rainbow Blvd #823  Las Vegas  NV  89107  USA
Telephone  702 349 6103  Fax  702 989-0841  email  [email protected]
Leading Developer and Manufacturer of In Vivo and DNA Transfection
Kits, Transfection Reagents and Electroporation Delivery Products
Products > CHO Transfection Reagent (Chinese Hamster Ovary Cells)
General Lipoplex-mediated Transfection Mechanism of Action
Altogen Biosystems  848 Rainbow Blvd #823  Las Vegas  NV  89107  USA
Telephone  702 349 6103  Fax  702 989-0841  email  [email protected]
Leading Developer and Manufacturer of In Vivo and DNA Transfection
Kits, Transfection Reagents and Electroporation Delivery Products
Products > CHO Transfection Reagent (Chinese Hamster Ovary Cells)
CHO Transfection Kit Product Details
•
Two component formulation enhances lipid mediated transfection efficiency
•
Optimized easy-to-use transfection protocol provided for transfection of siRNA, DNA,
mRNA, and microRNA
•
Kit includes Transfection Enhancer reagent and recommended transfection protocol
•
High transfection efficacy in the presence of serum
•
Expand your RNAi application with a reagent optimized for delivery of both siRNA and
plasmid
•
Reproducible transfection results
•
Works well for standard reverse transfection and high-throughput applications
Developed and manufactured by Altogen Biosystems (www.Altogen.com)
Altogen Biosystems  848 Rainbow Blvd #823  Las Vegas  NV  89107  USA
Telephone  702 349 6103  Fax  702 989-0841  email  [email protected]
Leading Developer and Manufacturer of In Vivo and DNA Transfection
Kits, Transfection Reagents and Electroporation Delivery Products
Products > CHO Transfection Reagent (Chinese Hamster Ovary Cells)
Data
Figure 1. GAPD mRNA levels were quantified using real-time RT-PCR in the CHO cells transfected
with siRNAs targeting GAPD or non-silencing siRNA. Forty-eight hours post-transfection, the cells were
harvested and analyzed by real-time RT-PCR for GAPDH mRNA expression levels. Data were
normalized against the 18S rRNA signal. Control samples were either mock-transfected or untreated.
Values are normalized to untreated sample. Data are means ± SD (n=3).
Leading Developer and Manufacturer of In Vivo and DNA Transfection
Kits, Transfection Reagents and Electroporation Delivery Products
Products > CHO Transfection Reagent (Chinese Hamster Ovary Cells)
Data
Figure 2. Protein expression of GAPDH in CHO cells. DNA plasmid expressing GAPDH or siRNA targeting
GAPDH were transfected into CHO cells following Altogen Biosystems transfection protocol. At 72 hours posttransfection the cells were analyzed by Western Blot for protein expression levels (normalized by total protein,
10 µg of total protein loaded per each well). Untreated cells used as a negative control.
Leading Developer and Manufacturer of In Vivo and DNA Transfection
Kits, Transfection Reagents and Electroporation Delivery Products
Products > CHO Transfection Reagent (Chinese Hamster Ovary Cells)
CHO Transfection Kit Benefits
• Pre-optimized transfection protocol for CHO cell line
• Compatible with DNA, small RNA (siRNA, shRNA, miRNA), mRNA, and small protein complexing
• Free of serum and protein of animal origin
• Compatible with standard and reverse transfection methods (both protocols provided in the kit manual)
• Easy to use CHO transfection protocol ensures great performance with expedited experimental timeline
• Equally efficient for single or multiple transfections
• Can be used for transient transfection and development of stable CHO cell lines
• Bio-degradable after endocytosis
• Used for preclinical research worldwide
Altogen Biosystems  848 Rainbow Blvd #823  Las Vegas  NV  89107  USA
Telephone  702 349 6103  Fax  702 989-0841  email  [email protected]