Download ETP: Genetic Engineering Quiz

Genetic Engineering Quiz
Lab Part 2: Using PCR Reaction to Identify Bacteria
Multiple Choice
Identify the letter of the choice that best completes the statement or answers the question.
Figure 13–1
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1. What does Figure 13–1 show?
a. gel electrophoresis
b. DNA sequencing
c. a restriction enzyme producing a DNA fragment
d. polymerase chain reaction
2. In Figure 13–1, between which nucleotides is the DNA cut?
a. adenine and thymine
c. thymine and cytosine
b. cytosine and guanine
d. adenine and guanine
3. Analyzing DNA by gel electrophoresis allows researchers to
a. identify similarities and differences in the genomes of different kinds of
organisms.
b. determine whether a particular allele of a gene is dominant or recessive.
c. compare the phenotypes of different organisms.
d. cut DNA with restriction enzymes.
4. On an electrophoresis gel, band B is closer to the positive end of the gel than is band A. Which of
the following statements is true?
a. Band B is more negatively charged than band A.
b. Band B moved faster than band A.
c. Band A is smaller than band B.
d. Band B consists of larger DNA fragments than does band A.
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5. Genetic engineering involves
a. reading a DNA sequence.
b. editing a DNA sequence.
c. reinserting DNA into living organisms.
d. all of the above
6. Which of the following are NOT used to read DNA sequences?
a. nucleotides
c. fluorescent dyes
b. gels
d. double-stranded DNA molecules
7. If two DNA samples showed an identical pattern and thickness of bands produced by gel
electrophoresis, the samples contained
a. the same amount of DNA.
c. the same DNA molecules.
b. fragments of the same size.
d. all of the above
8. Which of the following is often used as a genetic marker?
a. a foreign gene
b. a gene for antibiotic resistance
c. a DNA sequence that serves as a bacterial origin of replication
d. a nucleotide labeled with a fluorescent dye
9. Which of the following is an example of successful transformation?
a. injection of bacterial DNA into plant cells
b. a defective gene in a cell being replaced with a normal gene
c. bacterial cells taking in plasmids that have a genetic marker
d. none of the above
10. What is an advantage of using transgenic bacteria to produce human proteins?
a. The human proteins produced by transgenic bacteria work better than those
produced by humans.
b. Transgenic bacteria can produce human proteins in large amounts.
c. The human proteins produced by transgenic bacteria last longer than those
produced by humans.
d. Transgenic bacteria can produce human proteins used to make plastics.
11. Which of the following is a clone?
a. the adult female sheep whose DNA was used to produce Dolly
b. a transgenic mouse
c. a bacterium taken from a bacterial colony
d. the tobacco plant with the luciferase gene
Completion
Complete each sentence or statement.
12. The sequence of a DNA fragment can be determined by observing the ____________________ of
fluorescent bands on an electrophoresis gel.
13. A DNA sample will form a single band on an electrophoresis gel if all the fragments are the same
____________________.
14. The process of polymerase chain reaction is similar to the process of
_________________________, which occurs in cells.
15. The insulin produced by transgenic bacteria is identical to the insulin produced by humans because
both are coded for by ______________________________.
Short Answer
16. What is the likely effect of treating a sample of DNA with more than one kind of restriction
enzyme?
17. What does polymerase chain reaction enable scientists to make?
Figure 13–1
18. What are structures C and D in Figure 13–1, and what is their significance?
19. How can gel electrophoresis be used to tell whether a transformation experiment was successful?
Other
USING SCIENCE SKILLS
Figure 13–2
20. Interpreting Graphics In Figure 13–2, what do the bands shown in B consist of?
21. Interpreting Graphics Which group of bands in Figure 13–2 moved faster, C or D? Why?
22. Inferring In Figure 13–2, why are the bands in B moving toward the positive end of the gel?
23. Drawing Conclusions In Figure 13–2, were the three DNA samples shown in A identical? Explain
your answer.
Essay
24. Suppose you are a scientist trying to help people who cannot produce an enzyme needed for proper
digestion. How could you use genetic engineering techniques to make transformed bacteria that
produce the enzyme?
ETP: Genetic Engineering Quiz
Answer Section
MULTIPLE CHOICE
1. ANS:
STO:
2. ANS:
STO:
3. ANS:
STO:
4. ANS:
STO:
5. ANS:
STO:
6. ANS:
OBJ:
7. ANS:
STO:
8. ANS:
STO:
9. ANS:
STO:
10. ANS:
STO:
11. ANS:
STO:
C
BL.5.d
D
BL.5.a
A
BL.5.d
B
BL.5.d
D
BL.5.c
D
13.2.1
D
BL.5.d
B
BL.5
B
BL.5.d
B
BL.5.c
C
BL.4
DIF:
A
REF: p. 322
OBJ: 13.2.1
DIF:
A
REF: p. 322
OBJ: 13.2.1
DIF:
A
REF: p. 323
OBJ: 13.2.1
DIF:
E
REF: p. 323
OBJ: 13.2.1
DIF:
A
REF: p. 322
OBJ: 13.2.1
DIF: A
STO: BL.5.d
DIF: E
REF: p. 323, p. 324
REF: p. 323
OBJ: 13.2.1
DIF:
A
REF: p. 328
OBJ: 13.3.2
DIF:
E
REF: p. 329
OBJ: 13.3.2
DIF:
E
REF: p. 331
OBJ: 13.4.1
DIF:
A
REF: p. 333
OBJ: 13.4.2
REF: p. 323
OBJ: 13.2.1
STO: BL.5.d
DIF: E
REF: p. 323
14. ANS: DNA replication
OBJ: 13.2.1
STO: BL.5.d
DIF: E
REF: p. 325
15. ANS: the same gene DNA sequence
OBJ: 13.2.1
STO: BL.5.a, BL.5.b, BL.5.c
OBJ: 13.4.1
STO: BL.5.c
COMPLETION
12. ANS: colors
DIF: A
13. ANS: size
DIF:
E
REF: p. 331
SHORT ANSWER
16. ANS:
Treating a DNA sample with more than one kind of restriction enzyme will probably produce more
DNA fragments of different sizes.
DIF: E
REF: p. 323
OBJ: 13.2.1
STO: BL.5.d
17. ANS:
Polymerase chain reaction enables scientists to make many copies of a gene.
DIF: B
REF: p. 325
OBJ: 13.2.1
STO: BL.5.a, BL.5.b, BL.5.c
18. ANS:
Structures C and D are the sticky ends of a DNA fragment, which allow the fragment to be inserted
into a piece of DNA that has the same sticky ends.
DIF: A
REF: p. 322
OBJ: 13.3.1
STO: BL.5.b
19. ANS:
Gel electrophoresis can be used to compare banding patterns of DNA samples taken before and
after transformation was attempted. If the banding patterns are different, transformation was
successful.
DIF:
E
REF: p. 323
OBJ: 13.3.2
STO: BL.5.d
OTHER
20. ANS:
The bands consist of DNA fragments.
DIF: A
REF: p. 323
OBJ: 13.2.1
STO: BL.5.d
21. ANS:
The bands in group D moved faster because they consist of smaller DNA fragments.
DIF: A
REF: p. 323
OBJ: 13.2.1
22. ANS:
The bands consist of DNA, which is negatively charged.
STO: BL.5.d
DIF: A
REF: p. 323
OBJ: 13.2.1
STO: BL.5.d
23. ANS:
No, the DNA samples were not identical because they produced different patterns of bands on the
gel.
DIF:
ESSAY
A
REF: p. 323
OBJ: 13.2.1
STO: BL.5.d
24. ANS:
Extract DNA from the cells of people who can make the digestion enzyme. Cut the DNA with
restriction enzymes to cut out the gene that codes for the enzyme. Use gel electrophoresis to locate
the gene. Then, use polymerase chain reaction to make copies of the gene. Choose a plasmid that
has an antibiotic-resistance genetic marker, and cut the plasmid with the same restriction enzyme
used to cut out the human gene. Insert the copies of the human gene into the plasmids. Allow
bacterial cells to take in the plasmids. Select for transformed bacteria by growing them in a culture
containing the antibiotic. These bacteria will make the digestion enzyme.
DIF: E
OBJ: 13.3.1
REF: p. 323, p. 324, p. 325, p. 327, p. 328
STO: BL.5.c, BL.5.d, BL.5.e
LabPart 2: PCR Reaction to Identify Bacteria
From /ExamViewPro/Bank/PHBiology/ComputerTestBanks/Ch13; April 2005