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Appendix number 4 to the Senate Resolution No. 430/01/2015 SYLLABUS For the years 2015-2021 (date range) 1.1. BASIC INFORMATION CONCERNING THIS SUBJECT / MODULE Subject / Module Biochemistry with Elements of Chemistry Course code / module * BCh Faculty of (name of the leading direction) Faculty of Medicine, University of Rzeszow Department Name Department of Biochemistry Field of study Faculty of Medicine level of education Master Degree Studies Profile Practical Form of study Full Time and Part Time Year and semester 1st year, 1st semester Type of course Scientific Basis of Medicine Prof. Stanisław Wołowiec, Dr. hab. inż. Dorota Bartusik-Aebisher Coordinator Prof. Stanisław Wołowiec Dr. hab. inż. Dorota Bartusik-Aebisher Dr. Monika Stompor Dr. Rafał Podgórski First and Last Name of the Dr. David Aebisher Teachers Dr. Anna Pasternakiewicz Dr. Joanna Kisała Mgr. Tomasz Kubrak Mgr. Dorota Ulma-Tylek * - According to the resolutions of the Faculty of Medicine 1.2. Forms of classes, number of hours and ECTS Seminar Lecture Exercise Conversation Laboratory ZP 30 35 - - 25 - Practical Other - 1.3. The form of class activities X classes are in the traditional form ☐classes are implemented using methods and techniques of distance learning - Number of points ECTS 6.5 1.4. Examination Forms / module (exam, credit with grade or credit without grade) MODULE TRAINING- CREDIT WITH GRADE MODULE SEMINAR – CREDIT COMPLETED EXAM - CREDIT (ONE YEAR CLASS- EXAM AFTER THE SECOND SEMESTER) 2. REQUIREMENTS KNOWLEDGE OF CHEMISTRY AND BIOLOGY FROM HIGH SCHOOL WITH AN EXTENDED PROGRAM 3. OBJECTIVES, OUTCOMES, AND PROGRAM CONTENT USED IN TEACHING METHODS SUBJECT CONSISTS OF AN INITIAL PART WHICH AIMS TO PREPARE THE STUDENT TO USE CHEMICAL FORMULAS TO SUCH AN EXTENT THAT THE STUDENT HAS MASTERED THE LANGUAGE OF CHEMICAL FORMULAS AND CHEMICAL REACTIONS; CAN ATTRIBUTE PHYSICOCHEMICAL PROPERTIES TO A COMPOUND; HAS THE ABILITY TO PERFORM SIMPLE CHEMICAL CALCULATIONS AND THE ABILITY TO USE LABORATORY EQUIPMENT USING LABORATORY CHEMICAL AND BIOCHEMICAL COMPUNDS. THESE OBJECTIVES ARE TO BE ACHIEVED IN ONE SEMESTER THROUGH STUDENT PARTICIPATION IN LECTURES (10 HOURS OF GENERAL CHEMISTRY AND BIOCHEMISTRY 20 HOURS), SEMINAR CLASSES (25 HOURS) AND LABORATORY CLASSES (35 HOURS OF CLASSES IN CHEMISTRY). 3.1. Objectives of this course / module Understanding equilibrium, kinetics and thermodynamics of chemicals in aqueous solution C1 C2 Knowledge of the chemical formulas of amino acids, carbohydrates, lipids and their importance to physiology and the ability to diagram their metabolism C3 Ability to use laboratory equipment, performance of chemical and biochemical experiments according to the procedures described in the instructions for laboratory exercises C4 Ability to use the metabolic schemes (routes) in the field of synthesis and protein degradation pathways of carbohydrate, fat and fatty acids together with their regulation and disorders 3.2 OUTCOMES FOR THE COURSE / MODULE (TO BE COMPLETED BY THE COORDINATOR) EK (the effect of education) The content of learning outcomes defined for the class (module) EK_01 The student knows the basic reactions of inorganic and organic compounds in aqueous solution. The student knows and understands the concepts of solubility, osmotic pressure, ionization, colloidal solutions and Gibbs-Donnan Equilibrium EK_02 Student calculates the molar concentrations and percentage relationships and concentrations of substances in solution, iso-osmotic solutions, single- and multi-component solutions. Student calculates the solubility of inorganic compounds, chemical substrates and determines the solubility of organic compounds or lack thereof, and their practical significance for therapy. The student determines the pH of solutions and the impact of pH changes on organic and inorganic compounds Student provides the direction of biochemical processes depending on the energy states of cells. Student uses basic laboratory techniques such as: qualitative analysis, titration, colorimetry, pH, chromatography, electrophoresis of proteins and nucleic acids. EK_03 The student knows the structure of simple organic compounds including macromolecules present in cells, the extracellular matrix and body fluids. The student knows the structure of simple organic compounds included in the macromolecules present in cells, the extracellular matrix and body fluids. Student describes the structure of lipids and polysaccharides and their functions in cellular and extracellular structures. Student characterizes the structure of I-, II, III and quaternary proteins; knows functional post-translational modifications of proteins and their meaning. Student describes basic catabolic and anabolic pathways, their methods of regulation and the influence of genetic and environmental factors. The student knows the metabolic profiles of the basic organs and systems. 3.3 CONTENT CURRICULUM (filled by the coordinator) A. Lectures Course Contents LECTURES TP1. Scientific worldview. The history of the universe and of the Earth. Human populations. (1 hr.) TP2. Elements, their origin and circulation in nature. Compounds. (1 hr.) TP3. The solubility of compounds in water. Bonding – Energy, ionic interactions, covalent bonds, coordination chemistry, hydrogen bonds and van der Waals forces. Heterogeneous systems with a high degree of dispersion. (3 hrs.) TP4. Equilibria in water: hydration, dissociation, self-ionization of water, electrolytes, strong and weak acids and bases, acid-base balance, pH definition, equilibrium constant and degree of dissociation. The Henderson –Hasselbalch equation. Polyfunctional compounds - the acid-base properties of amino acids. Balance in the coordination sphere of metal ions. The pH of solutions of acids, bases, salts and buffers. (4 hrs.) B. TP5. The formal oxidation state. Redox reactions. Ion concentration transmembrane potential. Oxidation and reduction reactions in the body, redox potentials, the role of coenzymes and enzymes. (3 hrs.) TP6. Reaction kinetics, reaction order, rate equations. Catalysis, the role of catalysts. Radical reactions. (2 hours.) TP7. Thermodynamics. Heat and enthalpy. The first law of thermodynamics. The enthalpy of chemical transformations. Enthalpy as a function of state. Spontaneous transformations. Changes to the standard free energy of hydrolysis of key phosphates. The role of kinases and phosphatases. The relationship between the change in free energy, equilibrium constant and redox potential. (4 hrs.) TP8. Interaction of radiation with matter. Spectroscopy. Organic compounds (2 hrs.) TP9. Polypeptides and proteins. The life cycle of proteins. Determining the structure of primary proteins. The conformation of the protein backbone - Ramachandran plot. The structure of collagen. (2 hours.) TP10. Myoglobin and hemoglobin. The Bohr Cycle. (1 hr.) TP11. Enzymes. Classification of enzymes. Characteristics of the active site. Cofactors, coenzymes, prosthetic groups. Mechanisms of action of enzymes. Enzyme kinetics. The dependence of the MichaelisMenten equation and Hill equation. Lineweaver-Burk plots. Competitive and non-competitive inhibitors. Multi-substrate Reactions. Control of the amount and activity of enzymes. (4 hrs.) TP12. Bioenergetics. Glycolysis, metabolic syndromes. The energy balance (2 hrs.) TP13. Bioenergetics. Tricarboxylic acid cycle. (1 hr.) Problems, tutorials, conversational, laboratory practical classes Course Contents Laboratory Exercises TP2. (Exercise A1) demonstration classes and classrooms. Work safety in the chemical laboratory and biochemical laboratory (1 hr.). Demonstration of laboratory glassware and its applications, operation of the burner, centrifuges, pH-meter, pipetting techniques. Chemicals and solutions (3 hrs.). Total teaching time - 4 hours. TP3. TP4. (Exercise A2) Sparingly soluble compounds and their properties and complex ions (3 hours). TP5. (Exercise B3) Oxidation - reduction. (3 hrs.) TP3. TP4. (Exercise B4) Solutions: operations of weighing and measuring volume. Preparation of aqueous solutions of salts, acids and bases (3 hrs.). TP4. (Exercise B5) buffer solutions and acid-base indicators (3 hrs.). TP3. (Exercise B6) solutions, mixtures and their properties (3 hrs.). TP4. TP9. TP16. (Exercise B7) Organic compounds - synthesis reactions and methods of identification (3 hrs.). TP4. (Exercise C8) conductivity of electrolytes (3 hrs.). TP4. (Exercise C9) acid-base titration (3 hrs.) TP4. TP7. TP8. (Exercise C10) Chemical equilibrium - equilibrium constant, acid-base indicators - pKind (3 hrs.). TP8 (Exercise C11) Let's synthesize aspirin (3 hrs.). TP5. TP6. (Exercise 12) Kinetics and Catalysis (3 hrs.). A block of exercises performed by all students in the same class. Exercises in block B are performed in groups of two on a rotating basis (5 exercises in block B). Exercises in block C are done in groups of two on a rotating basis (5 exercises in block C). Each student must complete all the exercises. The condition of the execution of the exercise is a brief preliminary test. Performing exercises is confirmed on the basis of reporting on the scoreboard and the report credited by the teacher. Classes end with a final test covering all the content discussed in the exercises (1 hr.). SEMINAR TP1. TP2. Roots and building blocks of living matter (macronutrients), and elements present in small quantities and trace elements (micronutrients). Calculation of the molecular weights of compounds based on chemical formulas. The content of sodium and potassium ions in body fluids. Units of expression (1 hr.) TP3. Classes in the calculation of molar concentration of a compound (and its derivatives) in aqueous solution, dilution and drills in the calculation of the weight of a compound (and / or ion) contained in a solution of known concentration. (1 hr.) TP5. Exercise in determining the formal oxidation state of atoms in compounds consisting of C, H, O (ethane, ethanol, acetaldehyde, acetic acid, carbon dioxide). Exercise in determining the type of bond between elements of different electronegativity. (2 hours.) TP4. TP8. TP9. Organic compounds: saturated hydrocarbons, unsaturated and aromatic hydrocarbons. Alcohols, aldehydes, carboxylic acids. Amines and amides. Amino acids - equilibria in amino acids (2 hours). TP4. TP9. The calculation of the composition of a buffer solution for a predetermined pH. Buffers in the human body: the carbonate buffer, the buffer capacity of proteins (2 hrs.). TP7. Criterion of spontaneity of reaction. The relationship between the equilibrium constant of reaction enthalpy and free energy. (1 hr.) TP7. Electrochemical potential. Content supplementary lecture: Transport in passive and active membranes (1 hr.) Partial Test 1 (1 hr.). Content TP1. - TP5, TP8. TP9. + TP9.TP10.TP11. Protein metabolism. Features of proteins. Values of the pKa of amino acids and amino functional groups in the structure of proteins. (2 hours.) TP11. The active sites of enzymes and the regulation of enzyme activity. Inhibitors and activators of enzymes. (2 hours.) Content supplementary lecture: Transformation of amino acids in specialized products (2 hrs.) Partial Test 2 (1 hr.). Content TP9. - T11. TP12. Tp13. TP14. Bioenergetics: glycolysis, Krebs cycle, the pentosephosphate pathway (2 hrs.) TP16. TP17. The metabolism of fat and fatty acids (2 hours). Content supplementary lecture: Synthesis, transport and excretion of cholesterol (2 hrs.) Partial Test 3 (1 hr.). Content TP12. - T17. 3.4 TEACHING METHODS Lecture: Lecture with multimedia presentations Seminar: Problem Solving; discussion; self-development; a student presentation in biochemistry Laboratory: Gaining experience according to the procedures and design in laboratory 4 METHODS AND EVALUATION CRITERIA 4.1 Methods of verification of learning outcomes Symbol of Methods of assessment of learning outcomes (Eg.: effect tests, oral exams, written exams, project reports, observations during classes) EK_ 01 WRITTEN TEST 1 HOUR, AFTER 10 HOURS OF CLASSES AND SEMINARS EK_ 02 PRELIMINARY AND FINAL TESTS, REPORTS AND OBSERVATIONS DURING CLASSES EK_ 03 WRITTEN TEST 2 HOURS AFTER 6 HOURS OF CLASSES AND SEMINARS. WRITTEN TEST 3 HOURS AFTER 6 HOURS OF CLASSES AND SEMINARS Form of classes SEMINAR (LECTURE) LABORATORY EXERCISES SEMINAR (LECTURE) SEMINAR (LECTURE) 4.2 Conditions for completing the course (evaluation criteria) Lecture: Credit based on attendance. Seminar: Assessment based on partial written tests (3). Student is credited after all three tests are evaluated positively. The student has the right to make up one exam each term. If the student does not pass a second time, the student will have another chance at the end of the semester. The student has the right to two tests from all the material. In the absence of a positive assessment of the student has the right to apply to the Dean to ask for a test commission. Average grades of the tests will be taken into account at the final assessment of the whole subject at the end of the course. At the beginning of seminar students can expect pop quizes. Exercise: In order to receive credit from the laboratory, students must perform all the experiments contained in the program description, document the results, write conclusions in the report, and the report must be positively evaluated. The final evaluation of the exercises is the average of the partial grades of: pre-tests, final, lab execution and reports. 5. Total student workload required to achieve the desired result in hours and ECTS credits Activity Hours / student workload Hours of classes according to plan with the teacher 87 (30W; 22Sem; 35Lab) Preparation for classes 40 Participation in the consultations 2 The time to write a paper / essay 6 Preparation for tests 60 Participation in colloquia 3 Other (please specify) SUM OF HOURS 198 TOTAL NUMBER OF ECTS 6.5 6. TRAINING PRACTICES IN THE SUBJECT / MODUL Number of hours Rules and forms of apprenticeship 6. LITERATURE READING: "GENERAL CHEMISTRY; PARTICLES, MATTER, REACTIONS, "LORETTA JONES, PETER ATKINS. PWN W-WA 2004 (TRANS. JERZY KURYŁOWICZ) "BIOCHEMISTRY HARPER" RK MURRAY ET AL., PZWL, W-WA 2008 "CHEMICAL CALCULATIONS: A COLLECTION OF TASKS FROM GENERAL CHEMISTRY AND INORGANIC ANALYTICAL: COLLECTIVE WORK," ED. A. PLUMS. PWN W-WA, ANY EDITION. Additional literature: "Biochemistry" Lubert Stryer. PWN W-wa 1999 (trans. Collective). E. Bańkowski, Urban & Partner, Wrocław 2009. "Outline of physiological chemistry." Harold A. Harper, PZWL W-wa 1972 "General Chemistry with elements of biochemistry for students of medicine and science." Teresa Kedryn. Zamkor, 2005. "Medical Chemistry. Textbook for medical students. " Wladyslaw Galasiński, WZWL 2004. Acceptance Unit Manager or authorized person