Download Appendix number 4 to the Senate Resolution No. 430/01/2015

Appendix number 4 to the Senate Resolution No. 430/01/2015
SYLLABUS
For the years 2015-2021
(date range)
1.1.
BASIC INFORMATION CONCERNING THIS SUBJECT / MODULE
Subject / Module
Biochemistry with Elements of Chemistry
Course code / module *
BCh
Faculty of (name of the
leading direction)
Faculty of Medicine, University of Rzeszow
Department Name
Department of Biochemistry
Field of study
Faculty of Medicine
level of education
Master Degree Studies
Profile
Practical
Form of study
Full Time and Part Time
Year and semester
1st year, 1st semester
Type of course
Scientific Basis of Medicine
Prof. Stanisław Wołowiec, Dr. hab. inż. Dorota Bartusik-Aebisher
Coordinator
Prof. Stanisław Wołowiec
Dr. hab. inż. Dorota Bartusik-Aebisher
Dr. Monika Stompor
Dr. Rafał Podgórski
First and Last Name of the
Dr. David Aebisher
Teachers
Dr. Anna Pasternakiewicz
Dr. Joanna Kisała
Mgr. Tomasz Kubrak
Mgr. Dorota Ulma-Tylek
* - According to the resolutions of the Faculty of Medicine
1.2. Forms of classes, number of hours and ECTS
Seminar
Lecture Exercise Conversation Laboratory
ZP
30
35
-
-
25
-
Practical Other
-
1.3. The form of class activities
X classes are in the traditional form
☐classes are implemented using methods and techniques of distance learning
-
Number
of points
ECTS
6.5
1.4. Examination Forms / module (exam, credit with grade or credit without grade)
MODULE TRAINING- CREDIT WITH GRADE
MODULE SEMINAR – CREDIT
COMPLETED EXAM - CREDIT (ONE YEAR CLASS- EXAM AFTER THE SECOND
SEMESTER)
2. REQUIREMENTS
KNOWLEDGE OF CHEMISTRY AND BIOLOGY FROM HIGH SCHOOL WITH AN
EXTENDED PROGRAM
3. OBJECTIVES, OUTCOMES, AND PROGRAM CONTENT USED IN TEACHING
METHODS
SUBJECT CONSISTS OF AN INITIAL PART WHICH AIMS TO PREPARE THE STUDENT TO
USE CHEMICAL FORMULAS TO SUCH AN EXTENT THAT THE STUDENT HAS MASTERED
THE LANGUAGE OF CHEMICAL FORMULAS AND CHEMICAL REACTIONS; CAN
ATTRIBUTE PHYSICOCHEMICAL PROPERTIES TO A COMPOUND; HAS THE ABILITY TO
PERFORM SIMPLE CHEMICAL CALCULATIONS AND THE ABILITY TO USE
LABORATORY EQUIPMENT USING LABORATORY CHEMICAL AND BIOCHEMICAL
COMPUNDS. THESE OBJECTIVES ARE TO BE ACHIEVED IN ONE SEMESTER THROUGH
STUDENT PARTICIPATION IN LECTURES (10 HOURS OF GENERAL CHEMISTRY AND
BIOCHEMISTRY 20 HOURS), SEMINAR CLASSES (25 HOURS) AND LABORATORY
CLASSES (35 HOURS OF CLASSES IN CHEMISTRY).
3.1. Objectives of this course / module
Understanding equilibrium, kinetics and thermodynamics of chemicals in aqueous solution
C1
C2
Knowledge of the chemical formulas of amino acids, carbohydrates, lipids and their importance to
physiology and the ability to diagram their metabolism
C3
Ability to use laboratory equipment, performance of chemical and biochemical experiments according
to the procedures described in the instructions for laboratory exercises
C4
Ability to use the metabolic schemes (routes) in the field of synthesis and protein degradation
pathways of carbohydrate, fat and fatty acids together with their regulation and disorders
3.2 OUTCOMES FOR THE COURSE / MODULE (TO BE COMPLETED BY THE
COORDINATOR)
EK (the
effect of
education)
The content of learning outcomes defined for the class (module)
EK_01
The student knows the basic reactions of inorganic and organic
compounds in aqueous solution.
The student knows and understands the concepts of solubility, osmotic
pressure, ionization, colloidal solutions and Gibbs-Donnan Equilibrium
EK_02
Student calculates the molar concentrations and percentage relationships
and concentrations of substances in solution, iso-osmotic solutions,
single- and multi-component solutions.
Student calculates the solubility of inorganic compounds, chemical
substrates and determines the solubility of organic compounds or lack
thereof, and their practical significance for therapy.
The student determines the pH of solutions and the impact of pH
changes on organic and inorganic compounds
Student provides the direction of biochemical processes depending on
the energy states of cells.
Student uses basic laboratory techniques such as: qualitative analysis,
titration, colorimetry, pH, chromatography, electrophoresis of proteins
and nucleic acids.
EK_03
The student knows the structure of simple organic compounds including
macromolecules present in cells, the extracellular matrix and body
fluids.
The student knows the structure of simple organic compounds included
in the macromolecules present in cells, the extracellular matrix and body
fluids.
Student describes the structure of lipids and polysaccharides and their
functions in cellular and extracellular structures.
Student characterizes the structure of I-, II, III and quaternary proteins;
knows functional post-translational modifications of proteins and their
meaning.
Student describes basic catabolic and anabolic pathways, their methods
of regulation and the influence of genetic and environmental factors.
The student knows the metabolic profiles of the basic organs and
systems.
3.3 CONTENT CURRICULUM (filled by the coordinator)
A. Lectures
Course Contents
LECTURES
 TP1. Scientific worldview. The history of the universe and of the Earth.
Human populations. (1 hr.)
 TP2. Elements, their origin and circulation in nature. Compounds. (1
hr.)
 TP3. The solubility of compounds in water. Bonding – Energy, ionic
interactions, covalent bonds, coordination chemistry, hydrogen bonds
and van der Waals forces. Heterogeneous systems with a high degree
of dispersion. (3 hrs.)
 TP4. Equilibria in water: hydration, dissociation, self-ionization of
water, electrolytes, strong and weak acids and bases, acid-base
balance, pH definition, equilibrium constant and degree of
dissociation. The Henderson –Hasselbalch equation. Polyfunctional
compounds - the acid-base properties of amino acids. Balance in the
coordination sphere of metal ions. The pH of solutions of acids, bases,
salts and buffers. (4 hrs.)
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B.
TP5. The formal oxidation state. Redox reactions. Ion concentration
transmembrane potential. Oxidation and reduction reactions in the
body, redox potentials, the role of coenzymes and enzymes. (3 hrs.)
TP6. Reaction kinetics, reaction order, rate equations. Catalysis, the
role of catalysts. Radical reactions. (2 hours.)
TP7. Thermodynamics. Heat and enthalpy. The first law of
thermodynamics. The enthalpy of chemical transformations. Enthalpy
as a function of state. Spontaneous transformations. Changes to the
standard free energy of hydrolysis of key phosphates. The role of
kinases and phosphatases. The relationship between the change in
free energy, equilibrium constant and redox potential. (4 hrs.)
TP8. Interaction of radiation with matter. Spectroscopy. Organic
compounds (2 hrs.)
TP9. Polypeptides and proteins. The life cycle of proteins. Determining
the structure of primary proteins. The conformation of the protein
backbone - Ramachandran plot. The structure of collagen. (2 hours.)
TP10. Myoglobin and hemoglobin. The Bohr Cycle. (1 hr.)
TP11. Enzymes. Classification of enzymes. Characteristics of the active
site. Cofactors, coenzymes, prosthetic groups. Mechanisms of action
of enzymes. Enzyme kinetics. The dependence of the MichaelisMenten equation and Hill equation. Lineweaver-Burk plots.
Competitive and non-competitive inhibitors. Multi-substrate
Reactions. Control of the amount and activity of enzymes. (4 hrs.)
TP12. Bioenergetics. Glycolysis, metabolic syndromes. The energy
balance (2 hrs.)
TP13. Bioenergetics. Tricarboxylic acid cycle. (1 hr.)
Problems, tutorials, conversational, laboratory practical classes
Course Contents
Laboratory Exercises
 TP2. (Exercise A1) demonstration classes and classrooms.
 Work safety in the chemical laboratory and biochemical laboratory (1
hr.). Demonstration of laboratory glassware and its applications,
operation of the burner, centrifuges, pH-meter, pipetting techniques.
Chemicals and solutions (3 hrs.). Total teaching time - 4 hours.
 TP3. TP4. (Exercise A2) Sparingly soluble compounds and their
properties and complex ions (3 hours).
 TP5. (Exercise B3) Oxidation - reduction. (3 hrs.)
 TP3. TP4. (Exercise B4) Solutions: operations of weighing and
measuring volume. Preparation of aqueous solutions of salts, acids and
bases (3 hrs.).
 TP4. (Exercise B5) buffer solutions and acid-base indicators (3 hrs.).
 TP3. (Exercise B6) solutions, mixtures and their properties (3 hrs.).
 TP4. TP9. TP16. (Exercise B7) Organic compounds - synthesis reactions
and methods of identification (3 hrs.).
 TP4. (Exercise C8) conductivity of electrolytes (3 hrs.).
 TP4. (Exercise C9) acid-base titration (3 hrs.)
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TP4. TP7. TP8. (Exercise C10) Chemical equilibrium - equilibrium
constant, acid-base indicators - pKind (3 hrs.).
TP8 (Exercise C11) Let's synthesize aspirin (3 hrs.).
TP5. TP6. (Exercise 12) Kinetics and Catalysis (3 hrs.).
A block of exercises performed by all students in the same class.
Exercises in block B are performed in groups of two on a rotating basis (5
exercises in block B). Exercises in block C are done in groups of two on a rotating
basis (5 exercises in block C).
Each student must complete all the exercises. The condition of the execution
of the exercise is a brief preliminary test.
Performing exercises is confirmed on the basis of reporting on the scoreboard
and the report credited by the teacher.
Classes end with a final test covering all the content discussed in the exercises
(1 hr.).
SEMINAR

TP1. TP2. Roots and building blocks of living matter (macronutrients),
and elements present in small quantities and trace elements
(micronutrients). Calculation of the molecular weights of compounds
based on chemical formulas. The content of sodium and potassium
ions in body fluids. Units of expression (1 hr.)
 TP3. Classes in the calculation of molar concentration of a compound
(and its derivatives) in aqueous solution, dilution and drills in the
calculation of the weight of a compound (and / or ion) contained in a
solution of known concentration. (1 hr.)
 TP5. Exercise in determining the formal oxidation state of atoms in
compounds consisting of C, H, O (ethane, ethanol, acetaldehyde,
acetic acid, carbon dioxide). Exercise in determining the type of bond
between elements of different electronegativity. (2 hours.)
 TP4. TP8. TP9. Organic compounds: saturated hydrocarbons,
unsaturated and aromatic hydrocarbons. Alcohols, aldehydes,
carboxylic acids. Amines and amides. Amino acids - equilibria in amino
acids (2 hours).
 TP4. TP9. The calculation of the composition of a buffer solution for a
predetermined pH. Buffers in the human body: the carbonate buffer,
the buffer capacity of proteins (2 hrs.).
 TP7. Criterion of spontaneity of reaction. The relationship between
the equilibrium constant of reaction enthalpy and free energy. (1 hr.)
 TP7. Electrochemical potential. Content supplementary lecture:
Transport in passive and active membranes (1 hr.)
Partial Test 1 (1 hr.). Content TP1. - TP5, TP8. TP9. +
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TP9.TP10.TP11. Protein metabolism. Features of proteins. Values of
the pKa of amino acids and amino functional groups in the structure
of proteins. (2 hours.)
TP11. The active sites of enzymes and the regulation of enzyme
activity. Inhibitors and activators of enzymes. (2 hours.)
Content supplementary lecture: Transformation of amino acids in
specialized products (2 hrs.)
Partial Test 2 (1 hr.). Content TP9. - T11.

TP12. Tp13. TP14. Bioenergetics: glycolysis, Krebs cycle, the pentosephosphate pathway (2 hrs.)
 TP16. TP17. The metabolism of fat and fatty acids (2 hours).
 Content supplementary lecture: Synthesis, transport and excretion of
cholesterol (2 hrs.)
Partial Test 3 (1 hr.). Content TP12. - T17.
3.4
TEACHING METHODS
Lecture: Lecture with multimedia presentations
Seminar: Problem Solving; discussion; self-development; a student presentation in biochemistry
Laboratory: Gaining experience according to the procedures and design in laboratory
4 METHODS AND EVALUATION CRITERIA
4.1 Methods of verification of learning outcomes
Symbol of
Methods of assessment of learning outcomes (Eg.:
effect
tests, oral exams, written exams, project reports,
observations during classes)
EK_ 01
WRITTEN TEST 1 HOUR, AFTER 10 HOURS OF
CLASSES AND SEMINARS
EK_ 02
PRELIMINARY AND FINAL TESTS, REPORTS
AND OBSERVATIONS DURING CLASSES
EK_ 03
WRITTEN TEST 2 HOURS AFTER 6 HOURS OF
CLASSES AND SEMINARS.
WRITTEN TEST 3 HOURS AFTER 6 HOURS OF
CLASSES AND SEMINARS
Form of classes
SEMINAR
(LECTURE)
LABORATORY
EXERCISES
SEMINAR
(LECTURE)
SEMINAR
(LECTURE)
4.2 Conditions for completing the course (evaluation criteria)
Lecture: Credit based on attendance.
Seminar: Assessment based on partial written tests (3). Student is credited after all three tests
are evaluated positively. The student has the right to make up one exam each term. If the
student does not pass a second time, the student will have another chance at the end of the
semester. The student has the right to two tests from all the material. In the absence of a
positive assessment of the student has the right to apply to the Dean to ask for a test
commission. Average grades of the tests will be taken into account at the final assessment of
the whole subject at the end of the course. At the beginning of seminar students can expect
pop quizes.
Exercise: In order to receive credit from the laboratory, students must perform all the
experiments contained in the program description, document the results, write conclusions in
the report, and the report must be positively evaluated. The final evaluation of the exercises is
the average of the partial grades of: pre-tests, final, lab execution and reports.
5. Total student workload required to achieve the desired result in hours and ECTS credits
Activity
Hours / student workload
Hours of classes according to plan with the
teacher
87 (30W; 22Sem; 35Lab)
Preparation for classes
40
Participation in the consultations
2
The time to write a paper / essay
6
Preparation for tests
60
Participation in colloquia
3
Other (please specify)
SUM OF HOURS
198
TOTAL NUMBER OF ECTS
6.5
6. TRAINING PRACTICES IN THE SUBJECT / MODUL
Number of hours
Rules and forms of apprenticeship
6. LITERATURE
READING:
"GENERAL CHEMISTRY; PARTICLES, MATTER, REACTIONS, "LORETTA JONES, PETER ATKINS.
PWN W-WA 2004 (TRANS. JERZY KURYŁOWICZ)
"BIOCHEMISTRY HARPER" RK MURRAY ET AL., PZWL, W-WA 2008
"CHEMICAL CALCULATIONS: A COLLECTION OF TASKS FROM GENERAL CHEMISTRY AND
INORGANIC ANALYTICAL: COLLECTIVE WORK," ED. A. PLUMS. PWN W-WA, ANY EDITION.
Additional literature:
"Biochemistry" Lubert Stryer. PWN W-wa 1999 (trans. Collective).
E. Bańkowski, Urban & Partner, Wrocław 2009.
"Outline of physiological chemistry." Harold A. Harper, PZWL W-wa 1972
"General Chemistry with elements of biochemistry for students of medicine and
science." Teresa Kedryn. Zamkor, 2005.
"Medical Chemistry. Textbook for medical students. " Wladyslaw Galasiński,
WZWL 2004.
Acceptance Unit Manager or authorized person