Download Plasmids

Chapter 12
DNA Technology and Genomics
PowerPoint Lectures for
Campbell Biology: Concepts & Connections, Seventh Edition
Reece, Taylor, Simon, and Dickey
© 2012 Pearson Education, Inc.
Lecture by Edward J. Zalisko
Introduction
 DNA technology
– has rapidly revolutionized the field of forensics,
– permits the use of gene cloning to produce medical
and industrial products,
– allows for the development of genetically modified
organisms for agriculture,
– permits the investigation of historical questions about
human family and evolutionary relationships, and
– is invaluable in many areas of biological research.
© 2012 Pearson Education, Inc.
Figure 12.0_1
Chapter 12: Big Ideas
Gene Cloning
Genetically Modified
Organisms
DNA Profiling
Genomics
GENE CLONING
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12.1 Genes can be cloned in recombinant plasmids
 Biotechnology is the manipulation of organisms
or their components to make useful products.
 For thousands of years, humans have
– used microbes to make wine and cheese and
– selectively bred stock, dogs, and other animals.
 DNA technology is the set of modern techniques
used to study and manipulate genetic material.
© 2012 Pearson Education, Inc.
Figure 12.1A
12.1 Genes can be cloned in recombinant plasmids
 Genetic engineering involves manipulating
genes for practical purposes.
– Gene cloning leads to the production of multiple,
identical copies of a gene-carrying piece of DNA.
– Recombinant DNA is formed by joining nucleotide
sequences from two different sources.
– One source contains the gene that will be cloned.
– Another source is a gene carrier, called a vector.
– Plasmids (small, circular DNA molecules independent of the
bacterial chromosome) are often used as vectors.
© 2012 Pearson Education, Inc.
12.1 Genes can be cloned in recombinant plasmids
 Steps in cloning a gene
1. Plasmid DNA is isolated.
2. DNA containing the gene of interest is isolated.
3. Plasmid DNA is treated with a restriction enzyme that
cuts in one place, opening the circle.
4. DNA with the target gene is treated with the same
enzyme and many fragments are produced.
5. Plasmid and target DNA are mixed and associate with
each other.
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12.1 Genes can be cloned in recombinant plasmids
6. Recombinant DNA molecules are produced when
DNA ligase joins plasmid and target segments
together.
7. The recombinant plasmid containing the target gene
is taken up by a bacterial cell.
8. The bacterial cell reproduces to form a clone, a group
of genetically identical cells descended from a single
ancestral cell.
Animation: Cloning a Gene
© 2012 Pearson Education, Inc.
Figure 12.1B
E. coli bacterium
Plasmid
Bacterial
chromosome
1
A cell with DNA
containing the gene
of interest
2
A plasmid
is isolated.
The cell’s DNA
is isolated.
Gene of
interest
3
DNA
The plasmid is cut
with an enzyme.
Examples of gene use
4
The cell’s DNA is cut
with the same enzyme.
Gene
of interest
5
6
The targeted fragment
and plasmid DNA
are combined.
DNA ligase is added,
which joins the two
DNA molecules.
Examples of protein use
Recombinant
DNA
plasmid
Gene
of interest
7
The recombinant plasmid
is taken up by a bacterium
through transformation.
Recombinant
bacterium
8
Clone
of cells
Genes may be inserted
into other organisms.
The bacterium
reproduces.
9
Harvested
proteins
may be
used
directly.
Figure 12.1B_s1
E. coli
bacterium
Bacterial
chromosome
A cell with DNA
containing the gene
of interest
Plasmid
1
2
A plasmid
is isolated.
Gene of
interest
The cell’s DNA
is isolated.
DNA
Figure 12.1B_s2
E. coli
bacterium
Bacterial
chromosome
A cell with DNA
containing the gene
of interest
Plasmid
1
2
A plasmid
is isolated.
The cell’s DNA
is isolated.
Gene of
interest
3
DNA
The plasmid is cut
with an enzyme.
4
The cell’s DNA is cut
with the same enzyme.
Gene
of interest
Figure 12.1B_s3
E. coli
bacterium
Bacterial
chromosome
A cell with DNA
containing the gene
of interest
Plasmid
1
2
A plasmid
is isolated.
The cell’s DNA
is isolated.
Gene of
interest
3
DNA
The plasmid is cut
with an enzyme.
4
The cell’s DNA is cut
with the same enzyme.
Gene
of interest
5
The targeted fragment
and plasmid DNA
are combined.
Figure 12.1B_s4
E. coli
bacterium
Bacterial
chromosome
A cell with DNA
containing the gene
of interest
Plasmid
1
2
A plasmid
is isolated.
The cell’s DNA
is isolated.
Gene of
interest
3
DNA
The plasmid is cut
with an enzyme.
4
The cell’s DNA is cut
with the same enzyme.
Gene
of interest
5
6
Recombinant
DNA
plasmid
The targeted fragment
and plasmid DNA
are combined.
DNA ligase is added,
which joins the two
DNA molecules.
Gene
of interest
Figure 12.1B_s5
Recombinant
DNA
plasmid
Gene
of interest
7
Recombinant
bacterium
The recombinant plasmid
is taken up by a bacterium
through transformation.
Figure 12.1B_s6
Recombinant
DNA
plasmid
Gene
of interest
7
The recombinant plasmid
is taken up by a bacterium
through transformation.
8
The bacterium
reproduces.
Recombinant
bacterium
Clone
of cells
Figure 12.1B_s7
Genes may be inserted
into other organisms.
Recombinant
DNA
plasmid
Gene
of interest
7
The recombinant plasmid
is taken up by a bacterium
through transformation.
Recombinant
bacterium
8
Clone
of cells
The bacterium
reproduces.
9
Harvested
proteins
may be
used
directly.
12.2 Enzymes are used to “cut and paste” DNA
 Restriction enzymes cut DNA at specific
sequences.
– Each enzyme binds to DNA at a different restriction
site.
– Many restriction enzymes make staggered cuts that
produce restriction fragments with single-stranded
ends called “sticky ends.”
– Fragments with complementary sticky ends can
associate with each other, forming recombinant DNA.
 DNA ligase joins DNA fragments together.
Animation: Restriction Enzymes
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Figure 12.2_s1
1
DNA
Restriction enzyme
recognition sequence
A restriction
enzyme cuts
the DNA into
fragments.
2
Sticky
end
Restriction
enzyme
Sticky
end
Figure 12.2_s2
1
DNA
Restriction enzyme
recognition sequence
A restriction
enzyme cuts
the DNA into
fragments.
2
Sticky
end
A DNA fragment
from another
source is added.
Restriction
enzyme
Sticky
end
3
Gene of
interest
Figure 12.2_s3
1
DNA
Restriction enzyme
recognition sequence
A restriction
enzyme cuts
the DNA into
fragments.
2
Sticky
end
A DNA fragment
from another
source is added.
Two (or more)
fragments stick
together by
base pairing.
4
Restriction
enzyme
Sticky
end
3
Gene of
interest
Figure 12.2_s4
1
DNA
Restriction enzyme
recognition sequence
A restriction
enzyme cuts
the DNA into
fragments.
2
Sticky
end
A DNA fragment
from another
source is added.
Restriction
enzyme
Sticky
end
Gene of
interest
3
Two (or more)
fragments stick
together by
base pairing.
4
DNA ligase
pastes the
strands together.
5
DNA ligase
Recombinant
DNA molecule
12.3 Cloned genes can be stored in genomic
libraries
 A genomic library is a collection of all of the
cloned DNA fragments from a target genome.
 Genomic libraries can be constructed with
different types of vectors:
– plasmid library: genomic DNA is carried by plasmids,
– bacteriophage (phage) library: genomic DNA is
incorporated into bacteriophage DNA,
– bacterial artificial chromosome (BAC) library:
specialized plasmids that can carry large DNA
sequences.
© 2012 Pearson Education, Inc.
Figure 12.3
A genome is cut up with
a restriction enzyme
or
Recombinant
plasmid
Recombinant
phage DNA
Bacterial
clone
Phage
clone
Plasmid library
Phage library
12.4 Reverse transcriptase can help make genes
for cloning
 Complementary DNA (cDNA) can be used to
clone eukaryotic genes.
– In this process, mRNA from a specific cell type is the
template.
– Reverse transcriptase produces a DNA strand from
mRNA.
– DNA polymerase produces the second DNA strand.
© 2012 Pearson Education, Inc.
12.4 Reverse transcriptase can help make genes
for cloning
 Advantages of cloning with cDNA include the
ability to
– study genes responsible for specialized characteristics
of a particular cell type and
– obtain gene sequences
– that are smaller in size,
– easier to handle, and
– do not have introns.
© 2012 Pearson Education, Inc.
Figure 12.4
CELL NUCLEUS
Exon Intron
DNA of a
eukaryotic
gene
Exon
Intron Exon
1 Transcription
RNA
transcript
2 RNA splicing (removes
introns and joins exons)
mRNA
3 Isolation of mRNA from
TEST TUBE
Reverse transcriptase
cDNA strand
being synthesized
the cell and the addition
of reverse transcriptase;
synthesis of a DNA strand
4 Breakdown of RNA
Direction
of synthesis
5 Synthesis of second
DNA strand
cDNA of gene
(no introns)
Figure 12.4_1
CELL NUCLEUS
Exon Intron
DNA of a
eukaryotic
gene
Exon
Intron Exon
1 Transcription
RNA
transcript
2 RNA splicing (removes
introns and joins exons)
mRNA
Figure 12.4_2
3 Isolation of mRNA from
TEST TUBE
Reverse transcriptase
cDNA strand
being synthesized
the cell and the addition
of reverse transcriptase;
synthesis of a DNA strand
4 Breakdown of RNA
Direction
of synthesis
5 Synthesis of second
DNA strand
cDNA of gene
(no introns)
GENETICALLY MODIFIED
ORGANISMS
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12.6 Recombinant cells and organisms can
mass-produce gene products
 Recombinant cells and organisms constructed by
DNA technologies are used to manufacture many
useful products, chiefly proteins.
 Bacteria are often the best organisms for
manufacturing a protein product because bacteria
– have plasmids and phages available for use as genecloning vectors,
– can be grown rapidly and cheaply,
– can be engineered to produce large amounts of a
particular protein, and
– often secrete the proteins directly into their growth
medium.
© 2012 Pearson Education, Inc.
12.6 Recombinant cells and organisms can
mass-produce gene products
 Yeast cells
– are eukaryotes,
– have long been used to make bread and beer,
– can take up foreign DNA and integrate it into their
genomes,
– have plasmids that can be used as gene vectors, and
– are often better than bacteria at synthesizing and
secreting eukaryotic proteins.
© 2012 Pearson Education, Inc.
12.6 Recombinant cells and organisms can
mass-produce gene products
 Mammalian cells must be used to produce
proteins with chains of sugars. Examples include
– human erythropoietin (EPO), which stimulates the
production of red blood cells,
– factor VIII to treat hemophilia, and
– tissue plasminogen activator (TPA) used to treat heart
attacks and strokes.
© 2012 Pearson Education, Inc.
Table 12.6
12.6 Recombinant cells and organisms can
mass-produce gene products
 Pharmaceutical researchers are currently exploring
the mass production of gene products by
– whole animals or
– plants.
 Recombinant animals
– are difficult and costly to produce and
– must be cloned to produce more animals with the same
traits.
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Figure 12.6A
12.7 CONNECTION: DNA technology has
changed the pharmaceutical industry and
medicine
 Products of DNA technology are already in use.
– Therapeutic hormones produced by DNA technology
include
– insulin to treat diabetes and
– human growth hormone to treat dwarfism.
– DNA technology is used to
– test for inherited diseases,
– detect infectious agents such as HIV, and
– produce vaccines, harmless variants (mutants) or derivatives
of a pathogen that stimulate the immune system.
© 2012 Pearson Education, Inc.
Figure 12.7A
12.8 CONNECTION: Genetically modified
organisms are transforming agriculture
 Genetically modified (GM) organisms contain
one or more genes introduced by artificial means.
 Transgenic organisms contain at least one gene
from another species.
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12.8 CONNECTION: Genetically modified
organisms are transforming agriculture
 The most common vector used to introduce new
genes into plant cells is
– a plasmid from the soil bacterium Agrobacterium
tumefaciens and
– called the Ti plasmid.
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Figure 12.8A_s1
Agrobacterium
tumefaciens
DNA containing the
gene for a desired trait
Ti
plasmid
Restriction
site
1
The gene is
inserted into
the plasmid.
Recombinant
Ti plasmid
Figure 12.8A_s2
Agrobacterium
tumefaciens
DNA containing the
gene for a desired trait
Ti
plasmid
Restriction
site
1
The gene is
inserted into
the plasmid.
Plant cell
2
Recombinant
Ti plasmid
The recombinant
plasmid is
introduced into
a plant cell.
DNA carrying
the new gene
Figure 12.8A_s3
Agrobacterium
tumefaciens
DNA containing the
gene for a desired trait
Ti
plasmid
Restriction
site
1
The gene is
inserted into
the plasmid.
Plant cell
2
Recombinant
Ti plasmid
The recombinant
plasmid is
introduced into
a plant cell.
DNA carrying
the new gene
3
The plant cell
grows into
a plant.
A plant
with the
new trait
12.8 CONNECTION: Genetically modified
organisms are transforming agriculture
 GM plants are being produced that
– are more resistant to herbicides and pests and
– provide nutrients that help address malnutrition.
 GM animals are being produced with improved
nutritional or other qualities.
© 2012 Pearson Education, Inc.
Figure 12.8B
12.9 Genetically modified organisms raise concerns
about human and environmental health
 Scientists use safety measures to guard against
production and release of new pathogens.
 Concerns related to GM organisms include the
potential
– introduction of allergens into the food supply and
– spread of genes to closely related organisms.
 Regulatory agencies are trying to address the
– safety of GM products,
– labeling of GM produced foods, and
– safe use of biotechnology.
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12.10 CONNECTION: Gene therapy may
someday help treat a variety of diseases
 Gene therapy aims to treat a disease by
supplying a functional allele.
 One possible procedure is the following:
1. Clone the functional allele and insert it in a retroviral
vector.
2. Use the virus to deliver the gene to an affected cell
type from the patient, such as a bone marrow cell.
3. Viral DNA and the functional allele will insert into the
patient’s chromosome.
4. Return the cells to the patient for growth and division.
© 2012 Pearson Education, Inc.
12.10 CONNECTION: Gene therapy may
someday help treat a variety of diseases
 Gene therapy is an
– alteration of an afflicted individual’s genes and
– attempt to treat disease.
 Gene therapy may be best used to treat disorders
traceable to a single defective gene.
© 2012 Pearson Education, Inc.
Figure 12.10
Cloned gene
(normal allele)
1 An RNA version of
a normal human
gene is inserted
into a retrovirus.
RNA genome of virus
Retrovirus
2 Bone marrow cells
are infected with
the virus.
3 Viral DNA carrying the
human gene inserts into
the cell’s chromosome.
Bone marrow
cell from the patient
4 The engineered
cells are injected
into the patient.
Bone
marrow
Figure 12.10_1
Cloned gene
(normal allele)
1 An RNA version of
a normal human
gene is inserted
into a retrovirus.
RNA genome of virus
Retrovirus
Figure 12.10_2
2 Bone marrow cells
are infected with
the virus.
3 Viral DNA carrying the
human gene inserts into
the cell’s chromosome.
Bone marrow
cell from the patient
4 The engineered
cells are injected
into the patient.
Bone
marrow
12.10 CONNECTION: Gene therapy may
someday help treat a variety of diseases
 The first successful human gene therapy trial in
2000
– tried to treat ten children with SCID (severe combined
immune deficiency),
– helped nine of these patients, but
– caused leukemia in three of the patients, and
– resulted in one death.
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12.10 CONNECTION: Gene therapy may
someday help treat a variety of diseases
 The use of gene therapy raises many questions.
– How can we build in gene control mechanisms that
make appropriate amounts of the product at the right
time and place?
– How can gene insertion be performed without harming
other cell functions?
– Will gene therapy lead to efforts to control the genetic
makeup of human populations?
– Should we try to eliminate genetic defects in our
children and descendants when genetic variety is a
necessary ingredient for the survival of a species?
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DNA PROFILING
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12.11 The analysis of genetic markers can
produce a DNA profile
 DNA profiling is the analysis of DNA fragments to
determine whether they come from the same
individual. DNA profiling
– compares genetic markers from noncoding regions that
show variation between individuals and
– involves amplifying (copying) of markers for analysis.
© 2012 Pearson Education, Inc.
Figure 12.11
Crime scene Suspect 1
1
DNA is
isolated.
2 The DNA of
selected
markers is
amplified.
3
The amplified
DNA is
compared.
Suspect 2
12.12 The PCR method is used to amplify DNA
sequences
 Polymerase chain reaction (PCR) is a method of
amplifying a specific segment of a DNA molecule.
 PCR relies upon a pair of primers that are
– short,
– chemically synthesized, single-stranded DNA
molecules, and
– complementary to sequences at each end of the target
sequence.
 PCR
– is a three-step cycle that
– doubles the amount of DNA in each turn of the cycle.
© 2012 Pearson Education, Inc.
Figure 12.12
Cycle 1
yields two molecules
Genomic
DNA
3
5
Cycle 2
yields four molecules
5
3
5
3
separates
DNA
strands.
5
5
3
Target
sequence
5
2 Primers bond
1 Heat
3
3
polymerase
adds
nucleotides.
with ends
of target
sequences.
3
5
Primer
3
3 DNA
3
5
5
5
5
5
3
New DNA
Cycle 3
yields eight molecules
Figure 12.12_1
Cycle 1
yields two molecules
Genomic
DNA
3
5
1 Heat
3
5
5
3
separates
DNA
strands.
3
5
5
2 Primers bond
with ends
of target
sequences.
Target
sequence
3
5
3
3 DNA
polymerase
adds
nucleotides.
3
5
5
3
5
3
Primer
5
5
5
New DNA
3
Figure 12.12_2
Cycle 2
yields four molecules
Cycle 3
yields eight molecules
12.12 The PCR method is used to amplify DNA
sequences
 The advantages of PCR include
– the ability to amplify DNA from a small sample,
– obtaining results rapidly, and
– a reaction that is highly sensitive, copying only the
target sequence.
© 2012 Pearson Education, Inc.
12.13 Gel electrophoresis sorts DNA molecules
by size
 Gel electrophoresis can be used to separate
DNA molecules based on size as follows:
1. A DNA sample is placed at one end of a porous gel.
2. Current is applied and DNA molecules move from the
negative electrode toward the positive electrode.
3. Shorter DNA fragments move through the gel matrix
more quickly and travel farther through the gel.
4. DNA fragments appear as bands, visualized through
staining or detecting radioactivity or fluorescence.
5. Each band is a collection of DNA molecules of the
same length.
Video: Biotechnology Lab
© 2012 Pearson Education, Inc.
Figure 12.13
A mixture of DNA
fragments of
different sizes
Longer
(slower)
molecules
Power
source
Gel
Completed
gel
Shorter
(faster)
molecules
Figure 12.13_1
A mixture of DNA
fragments of
different sizes
Longer
(slower)
molecules
Power
source
Shorter
(faster)
molecules
Gel
Completed
gel
Figure 12.13_2
12.14 STR analysis is commonly used for DNA
profiling
 Repetitive DNA consists of nucleotide sequences
that are present in multiple copies in the genome.
 Short tandem repeats (STRs) are short nucleotide
sequences that are repeated in tandem,
– composed of different numbers of repeating units in
individuals and
– used in DNA profiling.
 STR analysis
– compares the lengths of STR sequences at specific sites
in the genome and
– typically analyzes 13 different STR sites.
© 2012 Pearson Education, Inc.
Figure 12.14A
STR site 1
STR site 2
Crime scene
DNA
The number of short
tandem repeats match
Suspect’s
DNA
The number of short tandem
repeats do not match
Figure 12.14B
Crime
scene
DNA
Suspect’s
DNA
Longer STR fragments
Shorter STR fragments
12.15 CONNECTION: DNA profiling has provided
evidence in many forensic investigations
 DNA profiling is used to
– determine guilt or innocence in a crime,
– settle questions of paternity,
– identify victims of accidents, and
– probe the origin of nonhuman materials.
© 2012 Pearson Education, Inc.
12.16 RFLPs can be used to detect differences in
DNA sequences
 A single nucleotide polymorphism (SNP) is a
variation at a single base pair within a genome.
 Restriction fragment length polymorphism
(RFLP) is a change in the length of restriction
fragments due to a SNP that alters a restriction
site.
 RFLP analysis involves
– producing DNA fragments by restriction enzymes and
– sorting these fragments by gel electrophoresis.
© 2012 Pearson Education, Inc.
Figure 12.16
Restriction
enzymes
added
DNA sample 1
DNA sample 2
w
Cut
z
x
Cut
Cut
y
Longer
fragments
y
Sample
1
Sample
2
z
x
Shorter
fragments
w
y
y
Figure 12.16_1
Restriction
enzymes
added
DNA sample 1 DNA sample 2
w
Cut
z
x
Cut
y
Cut
y
Figure 12.16_2
Longer
fragments
Sample
1
Sample
2
z
x
Shorter
fragments
w
y
y
GENOMICS
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12.17 Genomics is the scientific study of whole
genomes
 Genomics is the study of an organism’s complete
set of genes and their interactions.
– Initial studies focused on prokaryotic genomes.
– Many eukaryotic genomes have since been
investigated.
© 2012 Pearson Education, Inc.
Table 12.17
12.17 Genomics is the scientific study of whole
genomes
 Genomics allows another way to examine
evolutionary relationships.
– Genomic studies showed a 96% similarity in DNA
sequences between chimpanzees and humans.
– Functions of human disease-causing genes have been
determined by comparing human genes to similar
genes in yeast.
© 2012 Pearson Education, Inc.
12.18 CONNECTION: The Human Genome
Project revealed that most of the human
genome does not consist of genes
 The goals of the Human Genome Project (HGP)
included
– determining the nucleotide sequence of all DNA in the
human genome and
– identifying the location and sequence of every human
gene.
© 2012 Pearson Education, Inc.
12.18 CONNECTION: The Human Genome
Project revealed that most of the human
genome does not consist of genes
 Results of the Human Genome Project indicate that
– humans have about 20,000 genes in 3.2 billion
nucleotide pairs,
– only 1.5% of the DNA codes for proteins, tRNAs, or
rRNAs, and
– the remaining 98.5% of the DNA is noncoding DNA
including
– telomeres, stretches of noncoding DNA at the ends of
chromosomes, and
– transposable elements, DNA segments that can move or be
copied from one location to another within or between
chromosomes.
© 2012 Pearson Education, Inc.
Figure 12.18
Exons (regions of genes coding for protein
or giving rise to rRNA or tRNA) (1.5%)
Repetitive
DNA that
includes
transposable
elements
and related
sequences
(44%)
Introns and
regulatory
sequences
(24%)
Unique
noncoding
DNA (15%)
Repetitive
DNA
unrelated to
transposable
elements
(15%)
12.20 Proteomics is the scientific study of the full
set of proteins encoded by a genome
 Proteomics
– is the study of the full protein sets encoded by
genomes and
– investigates protein functions and interactions.
 The human proteome includes about 100,000
proteins.
 Genomics and proteomics are helping biologists
study life from an increasingly holistic approach.
© 2012 Pearson Education, Inc.
You should now be able to
1. Explain how plasmids are used in gene cloning.
2. Explain how restriction enzymes are used to “cut
and paste” DNA into plasmids.
3. Explain how plasmids, phages, and BACs are used
to construct genomic libraries.
4. Explain how a cDNA library is constructed and how
it is different from genomic libraries constructed
using plasmids or phages.
5. Explain how a nucleic acid probe can be used to
identify a specific gene.
© 2012 Pearson Education, Inc.
You should now be able to
6. Explain how different organisms are used to massproduce proteins of human interest.
7. Explain how DNA technology has helped to
produce insulin, growth hormone, and vaccines.
8. Explain how genetically modified (GM) organisms
are transforming agriculture.
9. Describe the risks posed by the creation and
culturing of GM organisms and the safeguards that
have been developed to minimize these risks.
© 2012 Pearson Education, Inc.
You should now be able to
10. Describe the benefits and risks of gene therapy in
humans. Discuss the ethical issues that these
techniques present.
11. Describe the basic steps of DNA profiling.
12. Explain how PCR is used to amplify DNA
sequences.
13. Explain how gel electrophoresis is used to sort
DNA and proteins.
14. Explain how short tandem repeats are used in
DNA profiling.
© 2012 Pearson Education, Inc.
You should now be able to
15. Describe the diverse applications of DNA
profiling.
16. Explain how restriction fragment analysis is
used to detect differences in DNA sequences.
17. Explain why it is important to sequence the
genomes of humans and other organisms.
18. Describe the structure and possible functions of
the noncoding sections of the human genome.
19. Explain how the human genome was mapped.
© 2012 Pearson Education, Inc.
You should now be able to
21. Compare the fields of genomics and proteomics.
22. Describe the significance of genomics to the
study of evolutionary relationships and our
understanding of the special characteristics of
humans.
© 2012 Pearson Education, Inc.
Figure 12.UN01
Bacterial
clone
Cut
Bacterium
DNA
fragments
Cut
Plasmids
Recombinant
DNA
plasmids
Recombinant
bacteria
Genomic library
Figure 12.UN02
A mixture of DNA
fragments
A “band” is a
collection of DNA
fragments of one
particular length
Longer
fragments
move slower
Shorter
fragments
move faster
DNA is attracted to 
pole due to PO4 groups
Power
source
Figure 12.UN03
DNA
amplified
via
(a)
Bacterial
plasmids
DNA
sample
treated with
treated with
(b)
DNA
fragments
sorted by size via
(c)
Recombinant plasmids
are inserted
into bacteria
Add
(d)
Particular
DNA
sequence
highlighted
are copied via
(e)
Figure 12.UN03_1
DNA
amplified
via
(a)
Bacterial
plasmids
DNA
sample
treated with
(b)
treated with
Figure 12.UN03_2
DNA
fragments
sorted by size via
(c)
Recombinant plasmids
are inserted
into bacteria
Add
(d)
Particular
DNA
sequence
highlighted
are copied via
(e)