Download Genetic Engineering Paper Exercise

Genetic Engineering Paper Exercise
You are provided with 2 DNA ‘samples’
Yellow = Human DNA
Cut out the two strips that make up the human DNA and stick them together to make
linear ‘DNA’. Stick tabs 1 and 2 together using the sticky labels.
Green = Bacterial plasmid DNA.
Join the tabs using the sticky labels to make a circle with the base sequence on the
outside.
Step 1: Isolation of human gene
The base sequence of the gene we wish to isolate is printed in bold letters.
Restriction enzymes are used to cut the DNA at specific base sequences.
You have a choice of 3 different restriction enzymes, which recognise specific base
sequences in the DNA, cutting the sugar-phosphate backbone at a particular point in
the sequence:
EcoRl
Hind III
SmaI
Which of these 3 recognition sequences are present in the human DNA?
Explain which restriction enzyme you would choose to isolate this gene.
Use scissors (restriction enzymes) to isolate the gene, making sure you make your cut
exactly as shown above.
Step 2 Insertion of gene into bacterial plasmid
Use the same restriction enzyme to cut open the bacterial plasmid.
Insert your isolated human gene into the plasmid, using sticky labels as DNA ligase.
Explain why the same restriction enzyme must be used. Explain why EcoR1 and Hind III
are more useful than Sma I for producing recombinant DNA.
Decide how you could adapt this paper model to show how antibiotic resistance genes
are used in genetic engineering.