Download dynamics of body and heart weights in the rats

DYNAMICS OF BODY AND HEART WEIGHTS IN THE RATS
AFTER АNTENATAL ANTIGEN INFLUENCE
Lebedinets A. N., Voloshin N. A., ReznichenkoYu.G.
Zaporozhye State Medical University, Ukraine
Abstract
Rationale There is a high prevalence of pathological pregnancy, intrauterine
infections of different etiology, accompanied by dysfunction of the placenta and the
penetration of foreign antigens to the fetus in the last decade. Pathology of the
antenatal period has negative health consequences over lifetime. Cardiovascular
pathology may be formed on the background of the heart morphogenesis disturbances
during fetal development. Intrauterine administration of antigens in experiment
causes acceleration of immunologically immature lymphocytes migration from the
thymus to the internal organs. These lymphocytes affect the formation of
morphological and functional units of the internal organs. Features of the
morphogenesis of heart after antenatal antigens exposure not studied.
Objective Determine the dynamics of the body and heart weights and their
ratio in experimental animals (rats) after antenatal antigens exposure.
Research
design
and
Methods
Experimental
study
of
the
heart
morphogenesis in rats of Wistar line after intrauterine injection of antigens at 18 days
of pregnancy by the method of Voloshin N.A. has performed. The dynamics of the
body and heart weights in the rats of six age groups (1, 7, 14, 30, 45 and 60 days of
postnatal period of ontogenesis) have investigated. Animals were divided in 4 groups:
I - intact group, II - intrauterine injection of Human Immunoglobulin for
intramuscular administration, III - intrauterine injection of Influenza Vaccine (split
virion, inactivated) VAXIGRIP, IV - intrauterine injection of Sodium Chloride
solution 0.9% (control group). Ratio of weight to length of the body was calculated.
Relative heart weight was determined by the formula: heart weight x 100, mg / body
weight, mg.
Results Increased body weight in animals of II and III groups compared with
body weight of intact (I) and control (IV) groups in all terms of postnatal period of
ontogenesis from the first day of life have noted. From 14 to 60 days increased body
weight in animals of groups II and III was significant as compared with indexes of
groups I and IV. There were no significant changes in the length of the body in
different groups of animals throughout the period of postnatal development. Ratio of
the weight and length of the body in the neonatal period and at 14, 30, 45 and 60 days
were significantly higher in animals of groups II and III in comparison with indexes
of intact and control groups. In intact and control animals groups were not detected
significant differences in body weight and length in any terms of postnatal period of
ontogenesis that excluded the impact of surgical intervention on the dynamics of the
body weight and length. The absolute heart weight had no significant differences in
the animals of different groups from 1 to 60 days of postnatal period, except for 7
days. The relative heart weight of animals groups II and III was significantly lower
than both the intact and control groups indexes from 7 days of life for all age groups
up to 60 days.
Conclusions Disproportion in development of rats hearts in experiment with
significant reduction of its relative weight at the period from 7 to 60 days of life on
the background trend in early terms and significant from 14 to 60 days of postnatal
development overweight of experimental animals have established. Detected changes
do not depend on the nature of the antigen and demonstrate the heart
morphofunctional disorders after antenatal antigens exposure. These features indicate
changes of the heart morphogenesis after an antenatal antigen influence that may
have essential contribution in the formation of cardiovascular diseases of children.
Further efforts should be directed to the study of the features of cardiovascular
system disorders in infants with antenatal antigen influence of foreign antigens.
Keywords: heart morphogenesis, rats, body weight, heart weight, antenatal
antigens exposure.