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Cell Organelles Wakefield 2016 Cells • Robert Hooke discovered the cell in the late 1600’s • The smallest part of any living organism • Contains all parts necessary to survive in changing environments Cells • Made primarily of H, O, C, &N • Though it also needs trace elements • Contains about 60% water • Intracellular and Interstitial • Main solid consists mainly of proteins Macromolecule Structure Carbohydrate – C, H, O Protein – C, H, O, N Lipid – C, H, O, P Nucleic Acids – C, H, O, N Water – H, O Cells • Cells are very diverse • Vary in length • 2 micrometers to more than 3 feet • Structure defines in function which are also very diverse Prokaryotic Cells vs. Eukaryotic Cells • All cells have generalized features: • Cytosol – semi-liquid fluid in which organelles are suspended – found in both pro & eukaryotic cells • Organelles – small organs of cell – most found only eukaryotic cells • Plasma Membrane – double layer of phospholipids which surrounds cell – found in both pro & eukaryotic cells • Inclusions – Non-functioning parts of cell NUCLEUS – CONTROL CENTER • Located near the center of the cell • Found in both plants & animals • Found only in eukaryotic cells • Contains the organism’s genetic material • The cell (and organism’s) “blueprint” • DNA – Deoxyribonucleic Acid • Chromatin • Genes • Chromosomes NUCLEOLUS – 1 OR MORE SMALL BODIES IN CENTRAL AREA OF NUCLEUS • Located near the center of nucleus • Found in both plants & animals • Found only in eukaryotic cells • Site for ribosome production which will migrate to cytoplasm NUCLEAR ENVELOPE – (aka) NUCLEAR MEMBRANE • Located surrounding the nucleus • Found in both plants & animals • Found only in eukaryotic cells • Consists of a double-layer selectively permeable membrane with relatively large pores (nuclear pores) • Serves to protect the nucleus and determines what enters & leaves nucleus CHROMATIN– DNA (when it is not dividing) • Located inside the nucleus • Found in both plants & animals • Found only in both prokaryotic and eukaryotic cells – just not within a membrane bound nucleus • Consists of DNA (Genetic Material) • Appears as loose bumpy granular material scattered throughout the nucleus CHROMOSOME – DNA (when it is dividing) • Located inside the nucleus • Found in both plants & animals • Found in both prokaryotic & eukaryotic cells • Consists of DNA (Genetic Material) • Appears as shortened thick structures resembling X’s or curved lines – condensed chromatin material CHROMASOME – CONDENSED CHROMATIN / DNA PLASMA MEMBRANE – (aka) CELL MEMBRANE • Surrounds the outside of the cell’s cytoplasm • Found in both plants & animals • Found only in eukaryotic cells & prokaryotic cells • Consists of phospholipid bi-layer • Functions to determine what enters and leaves the cell – is selectively permeable – selects which materials can pass across the membrane PLASMA MEMBRANE – (aka) CELL MEMBRANE • Specializations – • Microvilli – tiny fingerlike projections that greatly increase the cell’s surface area • Enhances cell’s absorption ability • Small intestines PLASMA MEMBRANE – (aka) CELL MEMBRANE • Specializations – • Membrane Junctions – varies structurally • Tight Junctions • Impermeable • Bind cells together like a zipper • Desmosomes • Anchoring junctions which prevent cells from tearing apart (i.e. – skin to skin or skin to muscle) PLASMA MEMBRANE – (aka) CELL MEMBRANE • Specializations – • Membrane Junctions – varies structurally • Gap Junctions • Connects adjacent cells with tubular connexons which look like hollow cylinders • Found in the heart, embryonic cells • Functions mainly in cell to cell communication Plasmodesmata – • Eukaryote / Plants • Sites for inter-cytoplasmic communication in plants • Similar to Gap Junctions in Animal Cells Nuclear Lamina – • Eukaryote / Plants & Animals • Inter-nuclear network of intermediate filaments and proteins providing mechanical support CYTOPLASM – • Located outside the nucleus and inside the plasma membrane • Found in both plants & animals • Found in prokaryotic & eukaryotic cells • Consists of 3 major elements • Cytosol • Organelles • inclusions CYTOPLASM – • 3 major elements of cytoplasm • Cytosol – semitransparent fluid which suspends the organelles • Organelles – “little organs” of cell where all cell functioning occurs • Inclusions – non-functioning units of the cell such as lipid droplets,, melanin (color pigment), etc. MITOCHONDRIA – (aka) POWERHOUSE OF CELL • In the cell’s cytoplasm • Found in both plants & animals • Found only in eukaryotic cells only • Consists of a double layer membrane surrounding “sausage-like” structures which can move and wriggle about • Functions to carry out the reactions which use O2 to break down food into cellular energy (ATP) • Found most in metabolically busy cells such as the liver & muscle cells RIBOSOME – cell’s protein factories • In the cell’s cytoplasm – tiny dark bilobed bodies of protein • Ribosomal RNA – (rRNA) • Some float free but most are attached to the Rough Endoplasmic Reticulum • Found in both plants & animals • Found in eukaryotic cells as well as prokaryotic cells • Not membrane bound in prokaryotic cells • Functions synthesize proteins for cell ENDOPLASMIC RETICULUM – (aka) ER • In the cell’s cytoplasm • Found in both plants & animals • Found only in eukaryotic cells ONLY • A “network” of tubules or “cisterns” • Accounts for about ½ of cell’s membranes • Functions to carry substances (primarily proteins) from 1 part of cell to another part of cell • 2 forms – • Rough ER & Smooth ER ROUGH ENDOPLASMIC RETICULUM – (aka) ROUGH ER Rough ER • Studded with ribosomes • Site of protein synthesis • Abundant in cells that export protein products • Pancreas – insulin & other digestive fluids SMOOTHENDOPLASMIC RETICULUM – (aka) SMOOTH ER Smooth ER • Plays no actual role in protein synthesis – • Communicates with the Rough ER & functions in lipid metabolism • Detoxifies drugs & pesticides • Abundant in: • Liver • Testes – to produce testosterone GOLGI APPARATUS – (aka) GOLGI BODY • Located in the cytoplasm of cell • Found in both plant & animal cells • Found in Eukaryotic cells only • Functions to modify, package and transport proteins • 2 types – • Transport Vesicles – modifies & packages proteins • Secretory Vesicles – transports proteins from Golgi Apparatus to plasma membrane LYSOSOME – (aka) cell’s demolition sites • Located in the cytoplasm of cell • Found in both plant & animal cells • Found in Eukaryotic cells only • Functions to breakdown other materials • Contain powerful “digestive” enzymes • “digest worn-out or non-usable” cells or cell parts • Exocytosis – transporting materials out of cell • Abundant in white blood cells • “digest” bacteria & other foreign substances PEROXISOME – • Located in the cytoplasm of cell • Found in both plant & animal cells • Found in Eukaryotic cells only • Contains powerful oxidase enzymes that use molecular O2 to detoxify harmful substances • Functions to disarm free radicals – highly reactive chemicals • Look like small lysosomes • Abundant in the liver & kidneys CYTOSKELETON – • Located in the cytoplasm of cell • Found in both plant & animal cells • Functions to provide form & structure to cells • Found in Eukaryotic cells only CYTOSKELETON – • Consists of 3 types of protein filaments • Intermediate filaments – help form desmosomes to provide internal guy wires to resist pulling forces • Microfilaments – such as actin & myosin – involved in cell motility and changes in cell shape (muscle cells) • Microtubules – determine the overall shape of the cell and the distribution of organelles • Important during cell division CENTRIOLES – • Located in the cytoplasm of cell • Found in both plant & animal cells • Found in Eukaryotic cells only • Rod-shaped bodies that lie at right angles to each other • Made up of fine microtubules and form the mitotic spindle during cell division • Some have cilia and/or flagella Cilia & Flagella – • Cilia - Microtubules used to move materials around outside of cell • Usually many of these small projections surrounding the cell or at least the exterior surface of a cell • Flagella – responsible for motility of the cell as a whole • Usually only one or a few and are long whip-like structures Central Vacuole– • Eukaryote / Plants • Function to give plant cells rigidity • Aka – turgidity • Can increase & decrease in size Chloroplast – • Eukaryote / Plants • Site for photosynthesis • Light & Dark Reaction • Hold chlorophyll • Inner Membranes Enclose: • Thylakoid / Granum • Stroma Lamellae • Intermembrane Space