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Interaction of
nanostructured silicon
with the living matter
Irina Kleps
National Institute for Research in Microtechnologies,
(IMT-Bucharest), P.O.Box 38-160, Bucharest,
Romania, fax: 4021.4908238, tel: 4021.4908412/33;
e-mail: [email protected]
MAIN PARTNERS
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Institute of Microtechnics, Mainz, Germany
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NMRC, Cork, Ireland
University of Bucharest, Faculty of
Chemistry
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Institute of Biochemistry, Bucharest
University of Bucharest, Faculty of Biology
Institute Chemico-Pharmaceutical,
Our team: Anca Angelescu, Mihaela Miu,
Monica Simion, Florea Craciunoiu, Teodora
Ignat, Adina Bragaru and Irina Kleps
Bucharest
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DEXTERCOM.SRL, Bucharest
Main results obtained in IMT-Bucharest –Laboratory of Nanotechnology
Cells behaviour
Nanostructured
silicon reactor;
Nanoelectrodes arrays
for electrochemical
/electrical measurements;
Lab-on-a-chip
Porous silicon
reservoirs for mineral or
drug delivery
PCR chip
Heating resistances
on the backside of
the chip can assure
a constant body fluid
temperature or other
temperature cycles
Nanoelectrode array - SEM details
r=5 nm
D=8 m
(a)
(b)
Some advantages of these electrodes:
(i) this kind of nanoelectrodes can be fabricated through the common photolithographic technique ultraviolet light proximity and does not involve other special lithographic techniques;
(ii) these electrodes are never recessed;
(iii) the array consists of a series of nanoelectrodes separated by an insulating material with
sufficient spacing to avoid the overlapping of the diffusion layers.
NE Biomedical applications: eukaryote and prokaryote
cells
ELECTROCHEMICAL CELL ON A CHIP
CHO cells on NE array
•investigation of the electrochemical
bacteria activity;
• study of the electron transfer from
living cells to the electrode arrays by
electrochemical measurements;
• conduction measurement on living cell
cultures;
Nanostructured silicon reactor for cell
investigation
Porous silicon (PS) reactor fabrication
PS stabilisation / functionalisation
Study of the prokaryotic or eukaryotic,
primary cells or cell lines behaviour in
interaction with PS
POROUS SILICON presents the advantage
over other bioactive materials because it is
compatible with silicon integrated circuit
technology
POROUS SILICON is a biocompatible and
bioactive substrate which facilitates the
adhesion and proliferation of various cell
The lab-on-a-chip with POROUS
SILICON reactor integrated with other
electrical and optical Si devices is an
useful tool to investigate cells answer to
various stimuli such as drug solutions,
electric impulses, or change of the
temperature
PS surface functionalisation
can be achieved by thermal,
chemical
and physico-chemical
treatments:
(i) thermal treatments in O2
(two steps process);
(ii) thin carbon, or silicon
carbide layer deposition;
(iii) surface derivatization
A
CHO cells on 31% PS / Sip+
(100), with the surface
modified by a
30nm carbon (10x)
PS/p+Si (100), 31% porosity,
annealed in N2 and
cultivated with B16-F1 cells
(20x);
Fluorescence images
obtained for CHO cell
lines grown on PS
surface
after 1 and 3 days
respectively
Resorbable porous silicon nanocomposite
reservoirs for mineral or drug delivery
Fe(NO3)3 deposition as powder,
followed by melting at 470C
Spin-on of a mixing etoxyetanol
and Fe(NO3)3
Samples with Fe / PS / Si
–n (cross section)
Fe release was investigated in SBF solution.
• In the first days a strong Fe release was observed
due to the dissolution of the iron salt from the PS
pores
•After that the iron release takes place in the same
time with the degradation of the PS matrix in SBF
solution.
DNA microchip
A silicon microchips with a
microreactor and a heater for
oligonucleotide amplification by
polymerase chain reaction - PCR and rapid
analysis of the DNA samples
Functionalized
reservoir with poly
(lysine).Fluorescent
primer immobilisation,
10 pmols / µl
Microfluidic Biochip for Biomedical Application
Recent published papers
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I. Kleps, A. Angelescu, M. Miu, M. Simion, A. Bragaru, M. Avram, Nanoelectrodes on silicon for electrochemical
applications, in NATO – ASI: Synthesis, Functional Properties and Applications of Nanostructures, (2002)
I. Kleps, A. Angelescu, M. Miu, Materials Science & Engineering C-Biomimetic and Supramolecular Systems; 19:219223 (2002).
Irina Kleps, Anca Angelescu, Marioara Avram, Mihaela Miu, Monica Simion, Microelectronic Engineering 61-62 (2002)
675-680.
Irina Kleps, "Electrochemical nanoelectrodes", in "Encyclopedia of Nanoscience and Nanotechnology", H. S. Nalwa,
(Ed.), American Scientific Publishers, 793-817, 2004.
A. Angelescu, I. Kleps, M. Miu, L. Zdrentu, S. Petrescu, M. Simion, A. Bragaru, T. Neghina,“Nanostructured silicon:
from material to devices for biomedical applications” “Nanoscience and Nanotechnology” 4, Heron Press, Sofia, 2004,
pp 280-283;
M. Miu, I. Kleps, A. Angelescu, T. Ignat, A. Bragaru, M. Simion, S. Petrescu, L. Zdrentu, N. Moldovan, M. Modreanu, P.
Rosengrave, “Porous silicon – a nanostructured biomaterial”, “Nanoscience and Nanoengineering” Vol., Series in “Micro
and Nanoengineering”, Romanian Academy Editure, Bucuresti 2004.
A. Angelescu, I. Kleps, M. Miu, S. Petrescu, C. Paduraru, M. Simion, A. Bragaru, A. Raducanu,“Thin carbon layers on
nanostructured silicon -properties and applications”, “Frontiers of Multifunctional Integrated Nanosystems”, Kluwer
Academic Publishers, 2004, 197-204.
M. Miu, A. Angelescu, I. Kleps, M. Simion, A. Bragaru, T. Neghina, M. Modreanu, D. Iacopino, P. Roseingrave,“Porous
silicon matrix as mineral microreservoir”, CAS 2003 Proceedings, vol. 1, 26th edition, oct. 8-12, 2003, p. 99-105;
A. Angelescu, I. Kleps, M. Miu, T. Neghina, M. Simion, A. Bragaru, M. Giurginca, „Metal-porous silicon composite layers
for biomedical applications”, „European Micro and Nanosystems 2004 (EMN04), Paris, Franta, 20-21 octombrie, 2004;
Monica Simion, Oana Tatiana Nedelcu, Irina Kleps, Anca Angelescu, Mihaela Miu,“Electro - Thermal Simulation For
PCR Silicon Chips”, Spring College on Science at Nanoscale , Trieste Italia