Download HYGROMYCIN B PRODUCT DESCRIPTION: MOLECULAR

PRODUCT DESCRIPTION:
Hygromycin B, an aminoglycoside antibiotic, inhibits the growth
of prokaryotic and eukaryotic cells. Specifically, it inhibits protein
synthesis by interfering with translocation of the 70S ribosome
subunit and inducing misreading of the mRNA template.
Hygromycin B has been used to select transfectants in a wide
variety of cells including bacteria, protozoans, yeast, fungi,
plants, and mammalian cells. Resistance to Hygromycin B is
conferred by a gene coding for a phosphotransferase that
phosphorylates Hygromycin B, thereby inactivating it.
Hygromycin B is an effective agent for the selection and
maintenance of bacterial and eukaryotic cells stably transfected
with vectors containing E. coli Hygromycin B resistance gene
(hyg or hph).
HYGROMYCIN B
Version 1.1.0
Catalog #03813
100 mg
MOLECULAR FORMULA: C20H37N3O13
MOLECULAR WEIGHT: 527.5
PHYSICAL DESCRIPTION: White powder.
SOLUBILITY: Soluble in aqueous solutions.
STORAGE:
The powder should be stored at 2 - 8°C. Solutions should be aliquoted and stored frozen at -20°C.
DIRECTIONS FOR USE:
This antibiotic is suitable for use in cell culture for the selection of hygromycin-resistant clones when using ClonaCell®-TCS medium
(Catalog #03814), and for the selection of any hygromycin-resistant transfected cell type. Product should be placed at room
temperature (15 - 25°C) for 30 minutes before use. Dissolve antibiotic in 1 mL of desired growth medium.
ClonaCell®-TCS Medium
Add to ClonaCell®-TCS medium at the concentration recommended in the Technical Manual (Catalog #28372). If you are selecting cell
lines not mentioned in the Technical Manual, please determine the optimal concentration of antibiotic needed.
The effective killing concentration of this antibiotic varies by cell type. Typical concentrations used for mammalian cell selection are
between 0.05 and 1 mg/mL.
If you are not using the entire solution, aliquot into working volumes and store at -20°C. Do not refreeze aliquots once they have been
thawed.
For more information on ClonaCell®-TCS, refer to the Technical Manual: http://www.stemcell.com/technical/manuals.aspx.
THIS REAGENT IS FOR RESEARCH ONLY.
NOT FOR THERAPEUTIC OR DIAGNOSTIC USE.
Revised:
April 2008