Journal of Biotechnology Rapid and highly efficient mammalian cell
... protein/gRNA RNP complexes were delivered into cultured human fibroblasts and induced pluripotent stem cells (iPSC) via electroporation with high efficiency and relatively low off-target effects (Kim et al., 2014). In that study, a large amount of Cas9 protein (4.5–45 g) and gRNA (6–60 g) were neces ...
... protein/gRNA RNP complexes were delivered into cultured human fibroblasts and induced pluripotent stem cells (iPSC) via electroporation with high efficiency and relatively low off-target effects (Kim et al., 2014). In that study, a large amount of Cas9 protein (4.5–45 g) and gRNA (6–60 g) were neces ...
Cell Biology IV:
... C4 photosynthesis Plants in dry and hot climates developed a modification of CO2 fixation which results in very low level of photorespiration (very high photosynthesis effciency) even when stomata are closed. Because the first product of CO2 fixation in such plants is a compound with four carbon ato ...
... C4 photosynthesis Plants in dry and hot climates developed a modification of CO2 fixation which results in very low level of photorespiration (very high photosynthesis effciency) even when stomata are closed. Because the first product of CO2 fixation in such plants is a compound with four carbon ato ...
RNA Amplification and cDNA Synthesis for qRT
... the No-RT control produced only nonspecific signals, as determined by the melting curve analysis in Fig. 2B. The melting temperature of the nonspecific products was significantly different from that of the LDHA-specific amplicon. Thus, the RNA amplification step, included in the MessageBOOSTER Kit r ...
... the No-RT control produced only nonspecific signals, as determined by the melting curve analysis in Fig. 2B. The melting temperature of the nonspecific products was significantly different from that of the LDHA-specific amplicon. Thus, the RNA amplification step, included in the MessageBOOSTER Kit r ...
... selenocystine reductase activity of TR in the presence of nonionic detergents such as NP40, which is a common nonionic detergent used in a wide variety of buffers to lyse mammalian cells. The use of NP-40 or other nonionic detergents inhibits activity in the original insulin end-point assay. Dr. Hon ...
Targets for breast cancer diagnosis and treatment
... vaccines-again” Scienti?c American (1994) Sep.:l02. Berns et al.. “Sporadic ampli?cation of the insulin-like growth factor 1 receptor gene in human breast tumors" Cancer Res. (1992) 52:1036-1039. Bishop. “Molecular themes in oncogenesis" Cell (1991) ...
... vaccines-again” Scienti?c American (1994) Sep.:l02. Berns et al.. “Sporadic ampli?cation of the insulin-like growth factor 1 receptor gene in human breast tumors" Cancer Res. (1992) 52:1036-1039. Bishop. “Molecular themes in oncogenesis" Cell (1991) ...
Akshaya Bio Inc.
... – Cellular (Class I)- critical to clear virus infected & cancer cells – Humoral (Class II)- Abs- Helps CTLs • Proof of principle established with Chimigen® HBV Therapeutic Vaccine • Potential use for both Prophylactic & Therapeutic Vaccines ...
... – Cellular (Class I)- critical to clear virus infected & cancer cells – Humoral (Class II)- Abs- Helps CTLs • Proof of principle established with Chimigen® HBV Therapeutic Vaccine • Potential use for both Prophylactic & Therapeutic Vaccines ...
Leukaemia Section Chronic myelogenous leukaemia (CML) Atlas of Genetics and Cytogenetics
... haematological relapse; 2-The most primitive stem cells seem to be resistant to IM therapy at least in vitro ; 3-The resistance to IM-therapy has been found to be associated, especially in patients who received it as second line treatment, with the occurrence of mutations in the ABL-kinase domain, i ...
... haematological relapse; 2-The most primitive stem cells seem to be resistant to IM therapy at least in vitro ; 3-The resistance to IM-therapy has been found to be associated, especially in patients who received it as second line treatment, with the occurrence of mutations in the ABL-kinase domain, i ...
The NaNo-INjecTIoN SySTem of cNIdarIa
... Dr. Tamar Lotan founded NanoCyte Inc, a biotech company which leverages the Cnidaria injection system for drug delivery. Prior to this, she was the R&D VP at Nidaria Technology. Her work in Nidaria led to the commercialization of the first jellyfish repellent product. Dr. Tamar Lotan is a specialist ...
... Dr. Tamar Lotan founded NanoCyte Inc, a biotech company which leverages the Cnidaria injection system for drug delivery. Prior to this, she was the R&D VP at Nidaria Technology. Her work in Nidaria led to the commercialization of the first jellyfish repellent product. Dr. Tamar Lotan is a specialist ...
bluebird bio, Inc. - corporate
... impurities that can include microvesicles. These cell-derived impurities can overlap key biophysical and biochemical attributes of the LVV, making them challenging to analyze. bluebird bio scientists reported on the use of various analytical tools to further characterize LVV preparations in terms ...
... impurities that can include microvesicles. These cell-derived impurities can overlap key biophysical and biochemical attributes of the LVV, making them challenging to analyze. bluebird bio scientists reported on the use of various analytical tools to further characterize LVV preparations in terms ...
Researcher Faked Evidence of Human Cloning, Koreans Report
... the future," the panel said. As for the field of embryonic stem cells, researchers in the United States say it should not be much affected in the long run, at least on a scientific level, since its theoretical promise is unchanged by one man's misdeeds. In practical terms, however, the panel's new f ...
... the future," the panel said. As for the field of embryonic stem cells, researchers in the United States say it should not be much affected in the long run, at least on a scientific level, since its theoretical promise is unchanged by one man's misdeeds. In practical terms, however, the panel's new f ...
- Horizon Discovery
... Figure 4. HaloTag® reporter images and pull-down. (A)-(C) Confocal experiments using SW48 wild type K-Ras HaloTag® cell lines show that K-Ras HaloTag® is present at the plasma membrane and in the cytoplasm. (A) KRas HaloTag® cells labelled with HaloTag® ligand show that the K-Ras HaloTag® fusion pro ...
... Figure 4. HaloTag® reporter images and pull-down. (A)-(C) Confocal experiments using SW48 wild type K-Ras HaloTag® cell lines show that K-Ras HaloTag® is present at the plasma membrane and in the cytoplasm. (A) KRas HaloTag® cells labelled with HaloTag® ligand show that the K-Ras HaloTag® fusion pro ...
UNIVERSITY OF MALTA
... thought to protect the hive and its occupants from microbial infection. Propolis has several reported medical applications in view of its antiseptic, antimycotic, antibacterial, antiviral, antiprotozoal and anti-inflammatory properties. Aims: The purpose of this study was to analyse the chemical con ...
... thought to protect the hive and its occupants from microbial infection. Propolis has several reported medical applications in view of its antiseptic, antimycotic, antibacterial, antiviral, antiprotozoal and anti-inflammatory properties. Aims: The purpose of this study was to analyse the chemical con ...
Abstract
... Recent breakthroughs in synthetic biology have led to the development of programmable bacteria— cells engineered to sense analytes in their environment (toxins, explosives, nitric oxide, pH) and execute specific programmed tasks. To harness the power of these microbes to assay and modify their envir ...
... Recent breakthroughs in synthetic biology have led to the development of programmable bacteria— cells engineered to sense analytes in their environment (toxins, explosives, nitric oxide, pH) and execute specific programmed tasks. To harness the power of these microbes to assay and modify their envir ...
When Wavelengths Collide: Bias in Cell Abundance Measurements
... metabolic burden imposed by various constructs being biased proportionally to mCherry expression level. We are unable to quantitatively estimate the bias in inferred construct activity reported in Ceroni et al. without repeating their experiments, but mitigating this bias by using OD700 to measure c ...
... metabolic burden imposed by various constructs being biased proportionally to mCherry expression level. We are unable to quantitatively estimate the bias in inferred construct activity reported in Ceroni et al. without repeating their experiments, but mitigating this bias by using OD700 to measure c ...
Introduction to Sierra Molecular Page 1 INTRODUCTION TO
... Molecular, will provide you with a concise, truncated overview of the Assay Assure technologies and their commercial applications. With worldwide addressable markets in diagnostics, pharmaceutical development, and basic research, the commercial opportunities for the AssayAssure® chemistries is diver ...
... Molecular, will provide you with a concise, truncated overview of the Assay Assure technologies and their commercial applications. With worldwide addressable markets in diagnostics, pharmaceutical development, and basic research, the commercial opportunities for the AssayAssure® chemistries is diver ...
Biotechnology, Plant Tissue Culture and Crop Improvement
... Introduction to Biotechnology : Definition, history, current demands from biological resources for food, fodder, feed, timber, oil, perfumes, pigments, biofertilizers, therapeutic agents, fermentation products etc. Bioprospecting. Ethical issues in biotechnology and safety and consumer issues of LMO ...
... Introduction to Biotechnology : Definition, history, current demands from biological resources for food, fodder, feed, timber, oil, perfumes, pigments, biofertilizers, therapeutic agents, fermentation products etc. Bioprospecting. Ethical issues in biotechnology and safety and consumer issues of LMO ...
PDF - Juno Therapeutics
... NEW YORK and SEATTLE, Dec. 4, 2013 /PRNewswire-USNewswire/ -- The Fred Hutchinson Cancer Research Center (Fred Hutch) and Memorial Sloan-Kettering Cancer Center (MSKCC), along with pediatric partner Seattle Children's Research Institute, have joined forces to launch Juno Therapeutics Inc., a new bio ...
... NEW YORK and SEATTLE, Dec. 4, 2013 /PRNewswire-USNewswire/ -- The Fred Hutchinson Cancer Research Center (Fred Hutch) and Memorial Sloan-Kettering Cancer Center (MSKCC), along with pediatric partner Seattle Children's Research Institute, have joined forces to launch Juno Therapeutics Inc., a new bio ...
Biology response 5 yeast
... Yeast, or the most commonly used Saccharomyces cerevisiae, is a tiny micro-organism that exists all around us - in soil, on plants, on fruit and even airborne. It has existed for so long, it is referred to as ‘the oldest plant cultivated by man’. Wild yeasts are single-celled microbes that are all a ...
... Yeast, or the most commonly used Saccharomyces cerevisiae, is a tiny micro-organism that exists all around us - in soil, on plants, on fruit and even airborne. It has existed for so long, it is referred to as ‘the oldest plant cultivated by man’. Wild yeasts are single-celled microbes that are all a ...
BIOL 463 Final Project 1st draft – Anik
... DNA methylation and histone markers associated with higher incidence for asthma Asthma is a heritable, multifaceted airway inflammatory disease afflicting approximately 8% of the USA population (Yang and Schwartz, 2012). The disease has steadily increased in prevalence and severity in the last coupl ...
... DNA methylation and histone markers associated with higher incidence for asthma Asthma is a heritable, multifaceted airway inflammatory disease afflicting approximately 8% of the USA population (Yang and Schwartz, 2012). The disease has steadily increased in prevalence and severity in the last coupl ...
0718 - a novel temperature-sensitive immortalized human adult
... Results and Discussion: Transfer to 39°C resulted in loss of TAg detected in cell extracts by Western blotting and complete inhibition of cell proliferation. After continuous monolayer culture at 32°C for 10 to 12 passages, passage through a 10-day alginate culture reestablished full phenotypic expr ...
... Results and Discussion: Transfer to 39°C resulted in loss of TAg detected in cell extracts by Western blotting and complete inhibition of cell proliferation. After continuous monolayer culture at 32°C for 10 to 12 passages, passage through a 10-day alginate culture reestablished full phenotypic expr ...
Mapping the radiobiological effectiveness of a pristine carbon beam
... To date, only a few studies have attempted to determine cellular responses other than cell killing along the ion path and they have been limited to very few positions (usually centre of the plateau and the peak) making it difficult to evaluate how the corresponding RBE varies across the ion trajecto ...
... To date, only a few studies have attempted to determine cellular responses other than cell killing along the ion path and they have been limited to very few positions (usually centre of the plateau and the peak) making it difficult to evaluate how the corresponding RBE varies across the ion trajecto ...
Pluristem - PR Increased Offering (January
... obtained by contacting H.C. Wainwright & Co., LLC, 430 Park Avenue, New York, NY 10022, by calling 646-975-6996 or by email at [email protected] or at the SEC's website at http://www.sec.gov. About Pluristem Therapeutics Pluristem Therapeutics Inc. is a leading developer of placenta-based cell th ...
... obtained by contacting H.C. Wainwright & Co., LLC, 430 Park Avenue, New York, NY 10022, by calling 646-975-6996 or by email at [email protected] or at the SEC's website at http://www.sec.gov. About Pluristem Therapeutics Pluristem Therapeutics Inc. is a leading developer of placenta-based cell th ...