Download Recombinant Human IL-35-Fc fusion protein (ELISA Std.)

Version: 3
Revision Date: 09/03/2014
Recombinant Human IL-35-Fc fusion protein (ELISA Std.)
Catalog# / Size
578502 / 10 µg
Other Names
IL-35 subunits: IL-12 subunit alpha (p35), IL-27 subunit
beta (EBI3)
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Description
IL-35 is a newly described cytokine comprised of the IL-12α chain (p35) and
the Epstein-Barr virus-induced gene product 3 (EBI-3). IL-35 belongs to the
IL-12 family which includes IL-12, IL-23, IL-27, and IL-35. These cytokines
are heterodimeric proteins comprised of an α chain (p19, p28, or p35) and a
β chain (p40 or Ebi3).
Mouse IL-35-Fc fusion protein enhances the proliferation of mouse Treg
cells, while inhibiting the development of Th17 cells. Human IL-35 can
suppress the proliferation of umbilical cord-derived human CD4 T
conventional cells, to a similar extent as activated Tregs. In addition,
activation of human CD4 T conventional cells with anti-CD3 and anti-CD28
in the presence of IL-35 induces the expression of IL-35 in these cells.
Interestingly, activated T conventional cells treated with IL-35 acquire a
regulatory phenotype (iTreg35) that suppress via IL-35. Most recent data
suggest that IL-35 secreted by ICOS+ Tregs can suppress IL-17 production
and reverse established IL-17-dependent allergic airways disease in mice.
Reactivity
Human
Source
Human IL-35 consists of two subunits linked via a 3x (SGGGG) bridge: EBI3 (Accession#
NM_005755.2) and p35 (Accession# NM_000882.3). Human IL-35 was expressed in 293E cells
as a human Fc-fusion protein (SP-EBI3-linker-p35-hFc).
Molecular Mass
The 762 amino acid recombinant protein has a predicted molecular weight of 84.4 kD. The non
-reduced migrates at approximately 200 kD, and the DTT-reduced protein migrates at
approximately 100 kD by SDS-PAGE.
Purity
>98%, as determined by Coomassie stained SDS-PAGE.
Formulation
0.22 µm filtered protein solution is in PBS.
Endotoxin Level
Less than 0.01 ng per µg cytokine as determined by the LAL method.
Preparation
10 µg size is bottled at 200 µg/mL.
Storage & Handling
Unopened vial can be stored between 2°C and 8°C for one month, at -20°C for six months, or at
-70°C for one year. For maximum results, quick spin vial prior to opening. The protein can be
aliquoted and stored from -20°C to -70°C. Stock solutions can also be prepared at 50-100 µg/mL
in sterile buffer (PBS, HPBS, DPBS, or EBSS) containing carrier protein such as 0.2-1% BSA or
HSA and stored in working aliquots at -20°C to -70°C. Avoid repeated freeze/thaw cycles.
Application
ELISA
Antigen Details
Structure
Heterodimer
Distribution
Regulatory T cells (Treg), CD4+ CD25+ Foxp3+ , CD4+ CD25+ , Foxp3- regulatory T cells.
Function
IL-35 suppresses T effector cell proliferation in vitro and induces a new subpopulation of Tregs
(iTregs35). IL-35-Fc fusion protein enhances the proliferation of mouse Treg cells. Interaction
between Tregs and T effector cells induces IL-35 production.
Interaction
T conventional cells, T regs.
Ligand Receptor
Unknown
Antigen References
1.
2.
3.
4.
5.
6.
Collison LW, et al. 2007. Nature 450:566.
Niedbala W, et al. 2007. Eur. J. Immunol. 37:3021.
Collison LW and Vignali DA. 2008. Immunol. Rev. 226:248.
Ito T, el al. 2008. Immunity 28:870.
Collison LW, et al. 2010. Nat. Immunol. 12:1093.
Whitehead GS, et al. 2012. J. Allergy Clin. Immunol. 129:207.
Gene ID
3592
10148
UniProt
View information about IL-35 on UniProt.org
Documentation
Technical data sheet
Certificate of Analysis
Safety Data Sheet
Related Protocols
Sandwich ELISA Protocol
Related FAQs
How do you convert activity as an ED50 in ng/ml to a specific activity in Units/mg?
Use formula Specific activity (Units/mg) = 10e6/ ED50 (ng/mL)
Related Categories
Cytokines & Chemokines > RecombinantProteins > Human > IL-35 >
Cytokines & Chemokines > Human Cytokine & Chemokine Research > Recombinant Proteins > IL-35 >
Antibodies and More > Cell Biology > Signaling/Signaling Intermediates > IL-35 >
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