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Download Tango INVESTIGATOR Name Stephen Crews Address
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Tango INVESTIGATOR Name Stephen Crews Address Dept. of Biochemistry, University of North Carolina at Chapel Hill, 321 Fordham Hall, CB#3280, Chapel Hill, NC 27599-3280 IMMUNOGEN Substance Name Origin Chemical Composition Developmental Stage IMMUNIZATION PROTOCOL Donor Animal Species Strain Sex Organ and tissue Immunization Dates immunized Amount of antigen Route of immunization Adjuvant FUSION Date Myeloma cell line Species Designation MONOCLONAL ANTIBODY Isotype Specificity Cell binding Immunohistology Antibody competition Species Specificity Tango amino acids 1-393 Tango Drosophila melanogaster bacterial GST fusion protein mouse BALB/C female spleen initial immunization in Summer 1996 and 6 additional injections every 21 days 50 g/injection subcutaneous Ribi 10/23/96 mouse P3x63 Ag8 653 IgG2a Drosophila ANTIGEN Tango Chemical properties protein Molecular weight 72 kDa (predicted) Characterization Immunoprecipitation yes Immunoblotting yes Purification Amino acid sequence analysis Functional effects Immunohistochemistry yes PUBLICATIONS : Sonnenfeld, M., Ward, M., Nystrom, G., Mosher, J., Stahl, S., and Crews, S. (1997). The Drosophila tango gene encodes a bHLHPAS protein that is orthologous to mammalian Arnt and controls CNS midline and tracheal development. Development 124, 45714582. Ward, M.P., Mosher, J.T., and Crews, S.T. (1998). Regulation of bHLH-PAS protein subcellular localization during Drosophila embryogenesis. Development 125, 1599-1608. ACKNOWLEDGMENTS STATEMENT We have been asked by NICHD to ensure that all investigators include an acknowledgment in publications that benefit from the use of the DSHB's products. We suggest that the following statement be used: “The (select: hybridoma, monoclonal antibody, or protein capture reagent,) developed by [Investigator(s) or Institution] was obtained from the Developmental Studies Hybridoma Bank, created by the NICHD of the NIH and maintained at The University of Iowa, Department of Biology, Iowa City, IA 52242.” Please send copies of all publications resulting from the use of Bank products to: Developmental Studies Hybridoma Bank Department of Biology The University of Iowa 028 Biology Building East Iowa City, IA 52242
