Download Cq4 INVESTIGATOR Name Elisabeth Knust Address Max

Cq4
INVESTIGATOR
Name
Elisabeth Knust
Address Max Planck Institute of Molecular Cell Biology and Genetics, Pfotenhauerstr. 108, Dresden 01307 Germany
E-mail: [email protected]
IMMUNOGEN
Substance
Name
Origin
Chemical Composition
Developmental Stage
IMMUNIZATION PROTOCOL
Donor Animal
Species
Strain
Sex
Organ and tissue
Immunization
Dates immunized
Amount of antigen
Route of immunization
Adjuvant
FUSION
Date
Myeloma cell line
Species
Designation
MONOCLONAL ANTIBODY
Isotype
Specificity
Cell binding
Immunohistology
Antibody competition
Species Specificity
ANTIGEN
Chemical properties
Molecular weight
Characterization
Fusion protein between -galactosidase and amino acid 737-1703 of Drosophila Crumbs
protein (cloned in pTRB vector). Expressed in bacteria, purified on PAA gel,
eluted and precipitated by acetone.
mouse
BALB/c
female
1990
1g
intraperitoneal
Freund’s complete, final boost: Freund’s incomplete
1991
mouse
NS-1
IgG1, kappa light chain
nd
nd
crossreacts with Drosophila virilis, not with mouse or C. elegans.
transmembrane protein
234 kDa
extracellular domain: 30 EGF-like repeats and 4 laminin-A G-domain like repeats
cytoplasmic domain: 37 amino acids, highly conserved in homologous protein of C. elegans
and human
Immunoprecipitation
Immunoblotting
Purification
Amino acid sequence analysis accession number: AAA28428
Functional effects
loss of crumbs function prevents stabilization of the zonula adherens of epithelial cells,
followed by the breakdown of the epithelial tissue structure and later cell death (whether Cq4
blocks this function has not been determined)
Immunohistochemistry
Crumbs protein is expressed in all epithelia of ectodermal origin in the Drosophila embryo from
gastrulation onwards and in all imaginal discs and the follicle epithelium. It is restricted to a
region apical to the zonula adherens. Good apical marker for epithelial cells.
PUBLICATIONS :
Tepass, U., Theres, C., and Knust, E. (1990). The Drosophila gene crumbs encodes an EGF-like protein, expressed on apical
membranes of epithelial cells and required for organization of epithelia. Cell 61, 787-799.
(Continued)
Cq4 (Continued)
Tepass, U., and Knust, E. (1990). Phenotypic and developmental analysis of mutations at the crumbs locus, a gene required for the
development of epithelia in Drosophila melanogaster. Roux’s Arch. Dev. Biol. 199, 189-206.
Tepass, U., and Knust, E. (1993). Crumbs and stardust act in a genetic pathway that controls the organization of epithelia in
Drosophila melanogaster. Dev. Biol. 159, 311-326.
Baumann, O. (2001). Posterior midgut epithelial cells differ in their organization of the membrane skeleton from other Drosophila
epithelia. Exp. Cell Res. 270, 176-187.
Morais-de-Sá, E., Mirouse, V., and St. Johnston, D. (2010). aPKC phosphorylation of Bazooka defines the apical/lateral border in
Drosophila epithelial cells. Cell 141, 509-523.
Maurel-Zaffran, C., Pradel, J., and Graba, Y. (2010). Reiterative use of signalling pathways controls multiple cellular events during
Drosophila posterior spiracle organogenesis. Dev. Biol. 343, 18-27
Galy, A., Schenck, A., Sahin, H.B., Qurashi, A., Sahel, J.-A., Diebold, C., and Giangrande, A. (2011). CYFIP dependent Actin
remodeling controls specific aspects of Drosophila eye morphogenesis. Dev. Biol. 359, 37-46.
ACKNOWLEDGMENTS STATEMENT
We have been asked by NICHD to ensure that all investigators include an acknowledgment in publications that benefit from the use of
the DSHB's products. We suggest that the following statement be used:
“The (select: hybridoma, monoclonal antibody, or protein capture reagent,) developed by [Investigator(s) or Institution] was
obtained from the Developmental Studies Hybridoma Bank, created by the NICHD of the NIH and maintained at The University
of Iowa, Department of Biology, Iowa City, IA 52242.”
Please send copies of all publications resulting from the use of Bank products to:
Developmental Studies Hybridoma Bank
Department of Biology
The University of Iowa
028 Biology Building East
Iowa City, IA 52242