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Customer Application Note Cancer Stem Cells Glioblastoma cancer stem cells elongate in VEGF-C surface gradient ClineNanoGradientswereusedtostudyhowagradientofsurface-boundVEGF-C affectsglioblastomacancerstemcells.StudywasperformedbyPetraSekyrova, PhD,KarolinskaInstituteSweden(2016). Glioblastomaisamostcommontypeofbraintumor.Itsunpredictableand aggressivebehaviorleadstoverypoortherapeuticoutcome.Tofindouthowto effectivelytargetglioblastomaitiscrucialtounderstandtheroleofglioblastoma cancerstemcells,whichareresponsibleforanunusualplasticityofthistumor. Laminin vs. Laminin/VEGF-C GlioblastomacancerstemcellsweregrownonVEGF-C/laminingradientsurface andtestedfortheabilitytorespondtoagradientofaVEGF-Cguidingfactor. GlioblastomacellswereobtainedfromtheHumanGlioblastomaCellCulture Bank,Uppsala,Sweden. A B A) Laminin-coated coverslip. B) VEGF-C and Laminin bound to Cline Nano Gradient. Higher concentration of VEGF-C in the upper part of the surface and lower concentration in the lower end of the surface. Results Cellselongatedandstretchedacrossatwotimeslongerdistancewithinthe VEGF-C/laminingradientcomparedtowhengrownonlyonlaminin. GlioblastomastemcellsgrownonaVEGF-C/laminingradientsurfaceretained theirstemnessproperties,asreflectedbySOX2andVimentinimmunostaining. Inconclusion,VEGF-C/laminingradientsurfaceallowedstudyingbehaviorof glioblastomacancerstemcellswithoutinterferingwiththeirstemness propertiesandcellswererespondingtotheVEGF-Cgradientbychangein morphology. ClineScientificAB www.ClineScientific.com +46313875555
