Survey
* Your assessment is very important for improving the workof artificial intelligence, which forms the content of this project
* Your assessment is very important for improving the workof artificial intelligence, which forms the content of this project
Customer Application Note Cancer Stem Cells Glioblastoma cancer stem cells elongate in VEGF-C surface gradient ClineNanoGradientswereusedtostudyhowagradientofsurface-boundVEGF-C affectsglioblastomacancerstemcells.StudywasperformedbyPetraSekyrova, PhD,KarolinskaInstituteSweden(2016). Glioblastomaisamostcommontypeofbraintumor.Itsunpredictableand aggressivebehaviorleadstoverypoortherapeuticoutcome.Tofindouthowto effectivelytargetglioblastomaitiscrucialtounderstandtheroleofglioblastoma cancerstemcells,whichareresponsibleforanunusualplasticityofthistumor. Laminin vs. Laminin/VEGF-C GlioblastomacancerstemcellsweregrownonVEGF-C/laminingradientsurface andtestedfortheabilitytorespondtoagradientofaVEGF-Cguidingfactor. GlioblastomacellswereobtainedfromtheHumanGlioblastomaCellCulture Bank,Uppsala,Sweden. A B A) Laminin-coated coverslip. B) VEGF-C and Laminin bound to Cline Nano Gradient. Higher concentration of VEGF-C in the upper part of the surface and lower concentration in the lower end of the surface. Results Cellselongatedandstretchedacrossatwotimeslongerdistancewithinthe VEGF-C/laminingradientcomparedtowhengrownonlyonlaminin. GlioblastomastemcellsgrownonaVEGF-C/laminingradientsurfaceretained theirstemnessproperties,asreflectedbySOX2andVimentinimmunostaining. Inconclusion,VEGF-C/laminingradientsurfaceallowedstudyingbehaviorof glioblastomacancerstemcellswithoutinterferingwiththeirstemness propertiesandcellswererespondingtotheVEGF-Cgradientbychangein morphology. ClineScientificAB www.ClineScientific.com +46313875555