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iBioSeminars: Bonnie Bassler, June 2008 Cell-Cell Communication in Bacteria, Part 1 Bacterial Quorum Sensing Part 1 Intra- and Inter-Species Communication Bonnie Bassler Princeton University HHMI The American Society for Cell Biology 1 iBioSeminars: Bonnie Bassler, June 2008 Cell-Cell Communication in Bacteria, Part 1 Quorum Sensing Low Cell Density High Cell Density Canonical Quorum Sensing Systems Gram-Negative Bacteria Gram-Positive Gram-Negative Autoinducers Gram-Positive The American Society for Cell Biology 2 iBioSeminars: Bonnie Bassler, June 2008 Cell-Cell Communication in Bacteria, Part 1 Canonical Quorum Sensing Systems Gram-Positive Bacteria Gram-Positive Autoinducers Quorum-Sensing-Controlled Genes V. fischeri Bioluminescence P.aeruginosa Virulence Factors, Biofilms A. tumefaciens Mating, Transfer of Mobile DNA E. carotovora Virulence Factors, Antibiotic Synthesis The American Society for Cell Biology 3 iBioSeminars: Bonnie Bassler, June 2008 Cell-Cell Communication in Bacteria, Part 1 Quorum Sensing in V. harveyi AI-2 LuxQ LuxP H D H LuxU H AI-1 D LuxN D HTH LuxO LuxCDABE Bacteria That Contain luxS (A sample of 299/534 genomes sequenced) Actinobacillus actinomycetemcomitans Bacillus anthracis Bacillus subtilis Borrelia burgdorferi Campylobacter jejuni Clostridium acetobutylicum Clostridium difficile Escherichia coli O157:H7 Enterococcus faecalis Haemophilus influenzae Klebsiella pneumoniae Neisseria gonorrhoeae Neisseria meningitidis Porphyromonas gingivalis Salmonella typhi Salmonella typhimurium Shigella flexneri Staphylococcus aureus Streptococcus pneumoniae Streptococcus pyogenes Vibrio cholerae Vibrio vulnificus Yersinia pestis The Borrelia Genome Project luxS metK pfs The American Society for Cell Biology 4 iBioSeminars: Bonnie Bassler, June 2008 Cell-Cell Communication in Bacteria, Part 1 SAM Utilization Met MetK SAM Methyltransferases SAH Pfs SRH 4,5-Dihydroxy+ Homocysteine 2,3 pentanedione SAM Utilization Met MetK SAM Methyltransferases SAH Pfs SRH LuxS 4,5-Dihydroxy+ 2,3 pentanedione Homocysteine The LuxS Reaction SRH LuxS 4,5-Dihydroxy+ Homocysteine 2,3-pentanedione The American Society for Cell Biology 5 iBioSeminars: Bonnie Bassler, June 2008 Cell-Cell Communication in Bacteria, Part 1 Quorum Sensing in V. harveyi AI-2 LuxQ LuxP H D H LuxU H AI-1 D LuxN D HTH LuxO LuxCDABE Holo-LuxP Holo-LuxP The American Society for Cell Biology 6 iBioSeminars: Bonnie Bassler, June 2008 Cell-Cell Communication in Bacteria, Part 1 Holo-LuxP AI-2 Formation in V. harveyi The American Society for Cell Biology 7 iBioSeminars: Bonnie Bassler, June 2008 Cell-Cell Communication in Bacteria, Part 1 The Salmonella Lsr Operon lsrA lsrC lsrD lsrB lsrF lsrG lsrE The Salmonella Lsr Operon LsrB LsrC LsrA LsrB LsrD periplasm cytoplasm Holo-LsrB The American Society for Cell Biology 8 iBioSeminars: Bonnie Bassler, June 2008 Cell-Cell Communication in Bacteria, Part 1 V. harveyi and Salmonella AI-2s V. harveyi S. typhimurium AI-2s are Interconverting Molecules LuxS product LuxP ligand AI-2s are Interconverting Molecules LuxS product LuxP ligand LsrB ligand The American Society for Cell Biology 9 iBioSeminars: Bonnie Bassler, June 2008 Cell-Cell Communication in Bacteria, Part 1 Genes Controlled by AI-2 Actinobacillus actinomycetemcomitans Bacillus anthracis Bacillus subtilis Borrelia burgdorferi Campylobacter jejuni Clostridium difficile Clostridium perfringens Escherichia coli K12 Escherichia coli EHEC O157:H7 Escherichia coli EPEC Vibrio harveyi Haemophilus influenzae Helicobacter pylori Klebsiella pneumoniae Lactobacillus reuteri Listeria monocytogenes Mannheimia haemolytica Neisseria meningitidis Photorhabdus luminescens Porphyromonas gingivalis Salmonella typhi Salmonella typhimurium Serratia marcescens Shigella flexneri Staphylococcus epidermis Streptococcus gordonii Streptococcus mutans Streptococcus pneumoniae Streptococcus pyogenes Vibrio cholerae Vibrio fischeri Vibrio harveyi Periodontal virulence, Mixed-species biofilms Growth Development Virulence Motility, Toxins Virulence, Toxins Virulence, Toxins AI-2 transport, Biofilms, Cell division, DNA processing, Iron uptake Virulence, Type III Secretion , Motility Motility, Type III Secretion Bioluminescence, Biofilms Virulence, Cell invasion Motility, Biofilms Biofilms Biofilms in mice Biofilms Virulence, Encapsulation, Adhesion Virulence, Bacteremia Antibiotic production Virulence, Proteases Hemin acquisition, Stress response Biofilms AI-2 transport, Biofilms Virulence in C. elegans, Hemolysin, Antibiotic production VirB (virulence factor) Virulence, Biofilms Mixed-species biofilms Virulence, Biofilms, Competence, Stress response Virulence, Persistence, Competence Virulence factors, Protease, Hemolysin Virulence, Toxins, Biofilms Bioluminescence, Colonization Bioluminescence, Biofilms, Siderophore, Virulence, Type III Secretion, Protease Quorum Sensing In Bacteria Bacteria Talk to Each Other Quorum Sensing In Bacteria Bacteria Talk to Each Other Multiple Languages i.e., intra- and inter-species communication The American Society for Cell Biology 10 iBioSeminars: Bonnie Bassler, June 2008 Cell-Cell Communication in Bacteria, Part 1 Quorum Sensing In Bacteria Bacteria Talk to Each Other Multiple Languages i.e., intra- and inter-species communication Bacteria Distinguish Self from Other Quorum Sensing In Bacteria Bacteria Talk to Each Other Multiple Languages i.e., intra- and inter-species communication Bacteria Distinguish Self from Other Many More Molecules Remain To Be Discovered i.e., molecules that tell “who” the other is Quorum Sensing In Bacteria Bacteria Talk to Each Other Multiple Languages i.e., intra- and inter-species communication Bacteria Distinguish Self from Other Many More Molecules Remain To Be Discovered i.e., molecules that tell “who” the other is Quorum Sensing Allows Bacteria To Be Multi-Cellular Similar to Higher Organisms The American Society for Cell Biology 11 iBioSeminars: Bonnie Bassler, June 2008 Cell-Cell Communication in Bacteria, Part 1 Quorum Sensing In Bacteria Bacteria Talk to Each Other Multiple Languages i.e., intra- and inter-species communication Bacteria Distinguish Self from Other Many More Molecules Remain To Be Discovered i.e., molecules that tell “who” the other is Quorum Sensing Allows Bacteria To Be Multi-Cellular Similar to Higher Organisms Opportunities for Novel Biotechnological Applications To Impede/Enhance Quorum Sensing Controlled Functions Quorum Sensing In Bacteria Bacteria Talk to Each Other Multiple Languages i.e., intra- and inter-species communication Bacteria Distinguish Self from Other Many More Molecules Remain To Be Discovered i.e., molecules that tell “who” the other is Quorum Sensing Allows Bacteria To Be Multi-Cellular Similar to Higher Organisms Opportunities for Novel Biotechnological Applications To Impede/Enhance Quorum Sensing Controlled Functions Natural Anti-Quorum Sensing Strategies Already Exist The American Society for Cell Biology 12