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Qty: 50 µg/ 200 µl Rabbit anti-Phosphoserine 608 Retinoblastoma Protein Catalog No. 34-1800 Lot No. See product label Rabbit anti-Phosphoserine 608 Retinoblastoma Protein FORM This polyclonal antibody is supplied as a 200 µl aliquot at a concentration of 0.25 mg/ml in phosphate buffered saline (pH 7.4) containing 0.1% sodium azide. The antibody is double epitope-affinity-purified from rabbit antiserum. PAD: Z99.SS IMMUNOGEN Synthetic peptide from human retinoblastoma protein containing phosphorylated serine 608. SPECIFICITY This antibody reacts with retinoblastoma protein phosphorylated at serine 608. There is no cross reactivity with unphosphorylated retinoblastoma protein. REACTIVITY Reactivity of this antibody was confirmed using human NALM-6 (pre B acute lymphoblastic leukemia) cells nuclear extract. Sample Human Mouse Western Blotting +++ NT Immunoprecipitation (native) +++ NT ELISA +++ NT (Excellent +++, Good++, Poor +, No reactivity 0, Not tested NT) USAGE Working concentrations for specific applications should be determined by the investigator. Appropriate concentrations will be affected by several factors, including secondary antibody affinity, antigen concentration, sensitivity of detection method, temperature and length of incubations, etc. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product. ELISA: 0.1-1.0 µg/ml Western Blotting: 1-5 µg/ml Immunoprecipitation: 10 µg/IP reaction STORAGE Store at 2-8°C for up to one month. Store at –20°C for long term storage. Avoid repeated freezing and thawing. (cont’d) www.invitrogen.com Invitrogen Corporation • 542 Flynn Rd • Camarillo • CA 93012 • Tel: 800.955.6288 • E-mail: [email protected] PI341800 (Rev 10/08) DCC-08-1089 Important Licensing Information - These products may be covered by one or more Limited Use Label Licenses (see the Invitrogen Catalog or our website, www.invitrogen.com). By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use. (34-1800 cont’d) BACKGROUND Entry into S phase from late G1 is controlled by phosphorylation of the retinoblastoma protein (Rb). Mutations affecting Rb or proteins that control Rb phosphorylation are frequent in human tumors. The cell cycle distribution of childhood acute lymphoblastic leukemia (ALL) cells shares many features with that of normal CD34+ progenitor cells. The majority of CD34+ and ALL cells reside in G1 phase although their cell cycle distribution within the G1 phase may be different. Entry of the cell into late G1 phase is controlled by phosphorylation of Rb. Different phosphoforms of Rb were recognized by their slower mobility on SDS-PAGE in ALL cells vs. CD34+ cells. In ALL cells, Rb was phosphorylated at ser-608, ser-780 and ser-795 unlike CD34+ cells. This evidence suggests that G1 phase distribution of ALL and CD34+ cells is different and that ALL cells progress to mid G1 phase while unstimulated CD34+ cells reside in early G1 phase. REFERENCES 1. Schmitz NMR et al; Phosphorylation of the Retinoblastoma Protein in Childhood Acute Lymphoblastic Leukemia Hematology 00:1-11 (2001) RELATED PRODUCTS Product Mouse anti-BrdU Mouse anti-p19SKP1 Mouse anti-p21CIP1/WAF1 Mouse anti-p27KIP1 Rabbit anti-phospho-p27KIP1 Mouse anti-PCNA PCNA Staining Kit BrdU Staining Kit Mouse anti-Retinoblastoma Mouse anti-Retinoblastoma Clone/PAD* ZBU30 PC19 EA10 p27-11D11 PT-187 PC10 Cat. No. 03-3900 71-9800 33-7000 33-2800 71-7700 13-3900 93-1143 93-3943 18-0159 28-0007 Rb1 MAb 1 ® Protein A rec-Protein G Sepharose 4B ® Sepharose 4B 10-1041 10-1241 *PAD: Polyclonal Antibody Designation Conjugate Purified FITC TRITC Cy™3 Cy™5 HRP AP Biotin ZyMAX™ Goat x Rabbit IgG (H+L) 81-6100 81-6111 81-6114 81-6115 81-6116 81-6120 81-6122 81-6140 ZyMAX™ Goat x Mouse IgG (H+L) 81-6500 81-6511 81-6514 81-6515 81-6516 81-6520 81-6522 81-6540 ® ® Zymed and ZyMAX™ are trademarks of Zymed Laboratories Inc. Cy™ is a trademark of Amersham Life Sciences, Inc. Sepharose is a registered trademark of Pharmacia LKB. For Research Use Only www.invitrogen.com Invitrogen Corporation • 542 Flynn Rd • Camarillo • CA 93012 • Tel: 800.955.6288 • E-mail: [email protected] PI341800 (Rev 10/08) DCC-08-1089 Important Licensing Information - These products may be covered by one or more Limited Use Label Licenses (see the Invitrogen Catalog or our website, www.invitrogen.com). By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.