Download (monclonal) Anti-Human Perforin Unconjugated

Mouse (monclonal) Anti-Human
Perforin Unconjugated
PRODUCT ANALYSIS SHEET
Catalog Number:
AHF0292
Lot Number:
See product label
Quantity/Volume:
100 µg/500 µL
Clone Number:
δG9
Isotype:
IgG2b kappa
Form of Antibody:
Purified immunoglobulin in 10 mM phosphate buffered saline, pH 7.4, with 0.2% BSA.
Preservation:
0.09% sodium azide (Caution: sodium azide is a poisonous and hazardous substance.
Handle with care and dispose of properly.)
Purification:
Purified from ascites by Protein A affinity chromatography.
Immunogen:
Purified granules from human YT lymphoma cell line.
Myeloma/Fusion
Partners:
BALB/c splenocytes fused with myeloma p3-NS-1/Ag4-1 cells.
Specificity:
This monoclonal antibody immunoprecipitates a protein with Mr=70 kDa known as
perforin or cytolysin. Perforin is a cytolytic pore-forming protein expressed by cytotoxic
T-lymphocytes (CTL) and natural killer cells (NK). This protein is located in the
cytoplasm and associated with granules. As with Fas ligand, perforin is a key effector in
T cell-mediated cytotoxicity which mediates cytolysis of target cells by membrane
damage and apoptosis.
There are several lines of evidence which indicate that this antibody is specific for
perforin. Sepharose-conjugated antibody preparations inactivate hemolytic granules
derived from cytotoxic T-lymphocytes or natural killer cells. In addition, transfection of
perforin-negative cells with perforin cDNA results in reactivity of these cells with this
antibody, and this reactivity can be reduced by the addition of partially purified perforin
preparations.
Species CrossReactivity:
Human. This antibody does not cross-react with mouse. Other species were not tested.
Applications:
This antibody is suitable for use in flow cytometry, immunofluorescence, and
immunohistochemistry with acetone fixed cryostat sections.
Suggested Working
Dilutions:
For immunoprecipitation, the use of 10 µL/mg protein lysate is recommended. For
immunohistochemistry, the use of 1:100-1:200 dilution is recommended with incubation
for 30-60 minutes at room temperature. The optimal antibody concentration should be
determined for each specific application.
This product is for research use only. Not for use in diagnostic procedures.
www.invitrogen.com
Invitrogen Corporation • 542 Flynn Rd • Camarillo • CA 93012 • Tel: 800.955.6288 • E-mail: [email protected]
PI AHF0292
(Rev 10/08) DCC-08-1089
Important Licensing Information - These products may be covered by one or more Limited Use Label Licenses (see the Invitrogen Catalog or our website, www.invitrogen.com). By
use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not
intended for human or animal diagnostic, therapeutic or commercial use.
Recommended Positive
Control:
Positive controls include YT cells, lymphokine activated killer cells (LAK), spleen, and
lymphoma.
Storage:
Store at 2-8°C. For long term storage, apportion into small aliquots and store at –20°C.
Avoid repeated freeze/thaw cycles to prevent denaturing the antibody.
Expiration Date:
Expires one year from date of receipt when stored as instructed.
References:
Briard, D. et al. (2002) Fibroblasts from human spleen regulate NK cell differentiation
from blood CD34(+) progenitors via cell surface IL-15. J. Immunol. 168(9):4326-4332.
Fox, W.M. et al. (1993) Perforin expression localizing cytotoxic lymphocytes in the
intimas of coronary arteries of transplant-related accelerated arteriosclerosis. Hum.
Pathol. 24(5):477-482.
Hameed. A. et al. (1992) Immunohistochemical identification of cytotoxic lymphocytes
using human perforin monoclonal antibody. Am. J. Pathol. 140(5):1025-1030.
Hameed, A. et al. (1996) Detection of perforin in human peritoneal fluid T-lymphocytes.
Acta Cytol. 40(3):401-407.
Rubes, G. et al. (1997) Increased perforin expression in multiple sclerosis patients during
exacerbation of disease in peripheral blood lymphocytes. J. Neuroimmunol. 74(1-2):198204.
Trapani, J.A. (1998) Dual mechanisms of apoptosis induction by cytotoxic lymphocytes.
Int. Rev. Cytol. 182:111-192.
Yamashita, Y. et al. (1998) Perforin and granzyme expression in cytotoxic T-cell
lymphomas. Mod. Pathol. 11(4):313-323.
This product is for research use only. Not for use in diagnostic procedures.
www.invitrogen.com
Invitrogen Corporation • 542 Flynn Rd • Camarillo • CA 93012 • Tel: 800.955.6288 • E-mail: [email protected]
PI AHF0292
(Rev 10/08) DCC-08-1089
Important Licensing Information - These products may be covered by one or more Limited Use Label Licenses (see the Invitrogen Catalog or our website, www.invitrogen.com). By
use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not
intended for human or animal diagnostic, therapeutic or commercial use.