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Mouse (monclonal) Anti-Human Perforin Unconjugated PRODUCT ANALYSIS SHEET Catalog Number: AHF0292 Lot Number: See product label Quantity/Volume: 100 µg/500 µL Clone Number: δG9 Isotype: IgG2b kappa Form of Antibody: Purified immunoglobulin in 10 mM phosphate buffered saline, pH 7.4, with 0.2% BSA. Preservation: 0.09% sodium azide (Caution: sodium azide is a poisonous and hazardous substance. Handle with care and dispose of properly.) Purification: Purified from ascites by Protein A affinity chromatography. Immunogen: Purified granules from human YT lymphoma cell line. Myeloma/Fusion Partners: BALB/c splenocytes fused with myeloma p3-NS-1/Ag4-1 cells. Specificity: This monoclonal antibody immunoprecipitates a protein with Mr=70 kDa known as perforin or cytolysin. Perforin is a cytolytic pore-forming protein expressed by cytotoxic T-lymphocytes (CTL) and natural killer cells (NK). This protein is located in the cytoplasm and associated with granules. As with Fas ligand, perforin is a key effector in T cell-mediated cytotoxicity which mediates cytolysis of target cells by membrane damage and apoptosis. There are several lines of evidence which indicate that this antibody is specific for perforin. Sepharose-conjugated antibody preparations inactivate hemolytic granules derived from cytotoxic T-lymphocytes or natural killer cells. In addition, transfection of perforin-negative cells with perforin cDNA results in reactivity of these cells with this antibody, and this reactivity can be reduced by the addition of partially purified perforin preparations. Species CrossReactivity: Human. This antibody does not cross-react with mouse. Other species were not tested. Applications: This antibody is suitable for use in flow cytometry, immunofluorescence, and immunohistochemistry with acetone fixed cryostat sections. Suggested Working Dilutions: For immunoprecipitation, the use of 10 µL/mg protein lysate is recommended. For immunohistochemistry, the use of 1:100-1:200 dilution is recommended with incubation for 30-60 minutes at room temperature. The optimal antibody concentration should be determined for each specific application. This product is for research use only. Not for use in diagnostic procedures. www.invitrogen.com Invitrogen Corporation • 542 Flynn Rd • Camarillo • CA 93012 • Tel: 800.955.6288 • E-mail: [email protected] PI AHF0292 (Rev 10/08) DCC-08-1089 Important Licensing Information - These products may be covered by one or more Limited Use Label Licenses (see the Invitrogen Catalog or our website, www.invitrogen.com). By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use. Recommended Positive Control: Positive controls include YT cells, lymphokine activated killer cells (LAK), spleen, and lymphoma. Storage: Store at 2-8°C. For long term storage, apportion into small aliquots and store at –20°C. Avoid repeated freeze/thaw cycles to prevent denaturing the antibody. Expiration Date: Expires one year from date of receipt when stored as instructed. References: Briard, D. et al. (2002) Fibroblasts from human spleen regulate NK cell differentiation from blood CD34(+) progenitors via cell surface IL-15. J. Immunol. 168(9):4326-4332. Fox, W.M. et al. (1993) Perforin expression localizing cytotoxic lymphocytes in the intimas of coronary arteries of transplant-related accelerated arteriosclerosis. Hum. Pathol. 24(5):477-482. Hameed. A. et al. (1992) Immunohistochemical identification of cytotoxic lymphocytes using human perforin monoclonal antibody. Am. J. Pathol. 140(5):1025-1030. Hameed, A. et al. (1996) Detection of perforin in human peritoneal fluid T-lymphocytes. Acta Cytol. 40(3):401-407. Rubes, G. et al. (1997) Increased perforin expression in multiple sclerosis patients during exacerbation of disease in peripheral blood lymphocytes. J. Neuroimmunol. 74(1-2):198204. Trapani, J.A. (1998) Dual mechanisms of apoptosis induction by cytotoxic lymphocytes. Int. Rev. Cytol. 182:111-192. Yamashita, Y. et al. (1998) Perforin and granzyme expression in cytotoxic T-cell lymphomas. Mod. Pathol. 11(4):313-323. This product is for research use only. Not for use in diagnostic procedures. www.invitrogen.com Invitrogen Corporation • 542 Flynn Rd • Camarillo • CA 93012 • Tel: 800.955.6288 • E-mail: [email protected] PI AHF0292 (Rev 10/08) DCC-08-1089 Important Licensing Information - These products may be covered by one or more Limited Use Label Licenses (see the Invitrogen Catalog or our website, www.invitrogen.com). By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.