Download 5e INVESTIGATOR Name Urs Rutishauser Address Memorial Sloan

5e
INVESTIGATOR
Name
Urs Rutishauser
Address Memorial Sloan-Kettering Cancer Center, Box 290, 1275 York Avenue, New York, NY 10021
IMMUNOGEN
Substance
Name
Origin
Chemical Composition
Developmental Stage
IMMUNIZATION PROTOCOL
Donor Animal
Species
Strain
Sex
Organ and tissue
Immunization
Dates immunized
Amount of antigen
Route of immunization
Adjuvant
FUSION
Date
Myeloma cell line
Species
Designation
MONOCLONAL ANTIBODY
Isotype
Specificity
Cell binding
Immunohistology
Antibody competition
Species Specificity
NCAM embryonic chicken brain membranes
approx E-14
mouse
Balb/c
female
spleen
3/12/83; 4/1/83; 4/20/83; 5/9/83
10-15 mg NCAM/mouse in PBS
i.p.
Freund’s complete followed by Freund’s incomplete
12/83
mouse
P3-X63-Ag8.653
IgG1, kappa
yes
yes
yes
chicken
ANTIGEN
Chemical properties
NCAM polypeptide (extracellular domain)
Molecular weight
180, 140 and 120 kDa
Characterization
Immunoprecipitation
yes
Immunoblotting
yes
Purification
yes
Amino acid sequence analysis
no
Functional effects
attenuates NCAM-NCAM binding
Immunohistochemistry
all NCAM positive tissues
PUBLICATIONS :
Watanabe, M., Frelinger, A.L., and Rutishauser, U. (1986). Topography of N-CAM structural and functional determinants. I.
classification of monoclonal antibody epitopes. J. Cell Biol. 103, 1721-1727.
Frelinger, A.L., and Rutishauser, U. (1986). Topography of N-CAM structural and functional determinants. II. placement of
monoclonal antibody epitopes. J. Cell Biol. 103, 1729-1737.
Brusés, J.L., and Rutishauser, U. (1998). Regulation of neural cell adhesion molecule polysialylation: evidence for
nontranscriptional control and sensitivity to an intracellular pool of calcium. J. Cell Biology 140(5), 1177-1186.
Goldstein, A.M., and Nagy, N. (2008). A bird’s eye view of enteric nervous system development: lessons from the avian embryo.
Pediatr. Res. 64, 326-333.
ACKNOWLEDGMENTS STATEMENT
We have been asked by NICHD to ensure that all investigators include an acknowledgment in publications that benefit from the use of
the DSHB's products. We suggest that the following statement be used:
“The (select: hybridoma, monoclonal antibody, or protein capture reagent,) developed by [Investigator(s) or Institution] was
obtained from the Developmental Studies Hybridoma Bank, created by the NICHD of the NIH and maintained at The University
of Iowa, Department of Biology, Iowa City, IA 52242.”
Please send copies of all publications resulting from the use of Bank products to:
Developmental Studies Hybridoma Bank
Department of Biology
The University of Iowa
028 Biology Building East
Iowa City, IA 52242