Download Microbial Fermentation - Quotation Inquiry Form Instructions Please

Microbial Fermentation - Quotation Inquiry Form
Instructions
Please answer as many questions as possible and email the completed form to [email protected].
You can also fax it to 1-732-210-0262 or 1-732-885-5878. Assuming all required information has been
provided, our service representative will get back to you with a free quote within 1 business day.
Customer Information
If you have registered an account with GenScript, please identify yourself by giving us your name and
email address or the Account No.
Name
Account No.
Phone
Organization
Shipping Address (necessary
to determine shipping cost)
Email Address
Target name*
Fermentation
details*
Click here to enter text.
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☐ Regular ☐ High cell density
Fermentation volume Click here to enter text.L
Need purified protein?
☐ Yes
☐ No
If “Yes”, list Yield Click here to enter text.mg
☐ Sequence Click here to enter text.
PurityClick here to enter text.%
Enter DNA or protein sequence. We will perform codon optimization & gene synthesis. Please
ensure sequence accuracy prior to submission. Due to formatting issues, occasionally, copypasting sequence directly into the sequence box may not be entirely accurate
Starting
material*
☐ DNA/Expression construct
We will subclone if required and transform into the appropriate strain
☐ Glycerol stock
☐ Plate
Bacteria
☐ E. coli
Fermentation
organism*
☐ Bacillus sps
Yeast
☐ S. cerevisiae
☐ Other Click here to enter text.
☐ P. pastoris
☐ Other Click here to enter text.
If “Other”, please enter full name of bacteria or yeast species. Please note that we only accept
non-sporulating, non-pathological strains for fermentation
Characterization
☐ Endotoxin ☐ ELISA
☐ Other ☐ None
☐ HPLC
☐ IEF
☐ N-terminal sequencing
SDS-PAGE and Western Blot will be used to determine fragment purity, A280 will be used to
measure the concentration and MALDI-TOF peptide mapping will be used to determine protein
identity (if required)
Protein Intended
Applications
(select all that
apply)
☐ Antigen Structural analysis ☐ Binding kinetics ☐ Display technologies
☐ Druggability studies ☐ Epitope Mapping ☐ Functionality studies
☐ Immunoassay ☐ Protein-receptor interaction studies ☐ Screening
☐ Other Click here to enter text.
Approximate
timeline for
project initiation
☐ ASAP
☐ Within 1 month
☐ Within 3 months
☐ Within 6 months
Click here to enter text.
Comments
Please enter any comments in this field
*Fields required in order to process your quote quickly
Terms and Conditions
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Standard deliverables include cell paste, batch records and QC datasheet. Synthetic gene in
pUC57 will be delivered if gene synthesis is performed
Gene synthesis and protein purification are add-on services that cost extra
If gene synthesis is performed, codon optimization using our proprietary, OptimumGene™
technology, will be performed in order to increase recombinant protein expression
We only accept non-sporulating, non-pathological strains for fermentation