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Table S1. Sensitivity and specificity of the commercial laboratory test kits used to test DENV, CHIKV and Rota/Adeno virus Kit (Manufacturer) Sensitivity Specificity Cross-reactivity Source of information NovaLisa Dengue Virus IgM- 90% 97.6% There is a cross reactivity among flavivirus due to presence of http://www.novatec- ELISA. (NovaTec common antigenic determinants. Bacterial contamination or freeze- id.com/products/infectious- Immundiagnostica GmbH, thaw cycles of the specimen may affect the absorbance values diseases/virology/dengue/ There is a cross reactivity among flavivirus due to presence of http://www.novatec- ELISA. (NovaTec common antigenic determinants. Bacterial contamination or freeze- id.com/products/infectious- Immundiagnostica GmbH, thaw cycles of the specimen may affect the absorbance values diseases/virology/dengue/ No cross-reactivity was observed by using Rheumatoid factor-samples http://www.novatec- capture ELISA (NovaTec and samples containing antibodies against Bordetella pertussis, id.com/products/infectious- Immundiagnostica GmbH, Chlamydia trachomatis, Chlamydia pneumoniae, Dengue Virus, TBE, diseases/virology/chikungunya-new/ Germany) Helicobacter pylori, HSV 2, Leishmania, Mycoplasma and Germany) NovaLisa Dengue Virus IgG- >90% 93% Germany) NovaLisa Chikungunya IgM μ- >90% >90% Schistosoma. Cross reactivity with antibodies against Borrelia, CMV and Toxoplasma cannot be excluded NovaLisa Chikungunya IgG Samples with IgG antibodies against Dengue virus, Tick born http://www.novatec- capture ELISA (NovaTec encephalitis, CMV, EBV, and Helicobacter pylori showed no cross id.com/products/infectious- Immundiagnostica GmbH, reactivity. Cross reactivity with antibodies against O’Nyong Nyong diseases/virology/chikungunya-new/ Germany) virus is not excluded RIDA® QUICK Rotavirus >90% 100% >90% 99% A positive result does not rule out the presence of another infectious http://www.r-biopharm.com/wp- pathogen. A negative result does not necessarily rule out rotavirus or content/uploads/items/ridaquick- adenovirus infection rotavirusadenovirus-combi-dipsticks3855/N1002-RIDAQUICK-Rota- Adeno-Box-10-08-10_GB.pdf RIDA® QUICK Adenovirus 90% 100% A positive result does not rule out the presence of another infectious http://www.r-biopharm.com/wp- pathogen. A negative result does not necessarily rule out rotavirus or content/uploads/items/ridaquick- adenovirus infection rotavirusadenovirus-combi-dipsticks3855/N1002-RIDAQUICK-RotaAdeno-Box-10-08-10_GB.pdf