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The B-box zinc finger family transcription factor, AtDBF1, is a positive regulator of abiotic stress tolerance through the ABA biosynthesis-mediated signaling pathway in Arabidopsis Ji-Hee Min and Cheol Soo Kim* Departments of Plant Biotechnology, Chonnam National University, Gwangju 500-757, South Korea Functional analysis of a putative novel transcription factor Arabidopsis B-box zinc finger family transcription factor protein designated AtDBF1, which contains predicted DNAbinding domain, was performed. The precise roles of the B-box zinc finger family transcription factors in plant stress are poorly understood. Analyses of an AtDBF1 promoterīĸ-glucuronidase (GUS) construct revealed substantial gene expression in whole seedling and expression of AtDBF1was strongly induced by abscisic acid (ABA), salt and osmotic stress treatments. atdbf1 mutant line was more sensitive to ABA and salt stress in the cotyledon greening processes. In contrast, AtDBF1-overexpressing plants were more insensitive to ABA and salt stress than the wild type. Interestingly, in the presence of ABA or salt stress, the transcript level of ABA biosynthesis and stress-related genes in AtDBF1-overexpressing plants was slightly higher than that in the wild type, whereas the expression of ABA biosynthesis genes in atdbf1 was significantly reduced. Thus, AtDBF1 is involved in ABA and salt stress response through the ABA biosynthesis-mediated signaling pathway. Taken together, these findings provide compelling evidence that AtDBF1 is an important regulator for plant tolerance to abiotic stress. * Email: [email protected]