Download and GABA in the outer part of rat retina following acute high

会员号：S424300153A
Immunoexpression of synaptophysin(SYN) and GABA in the
outer part of rat retina following acute high
intraocular pressure
*
Jia LUO , Hui WANG , Dan CHEN , Ju-Fang HUANG
Department of Anatomy and Neurobiology, Xiangya School of Medicine, Central South
University, Changsha, 410013.
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* Corresponding author: Dan CHEN
E-mail:[email protected]
Abstract: Objective To investigate the changes of synaptic plasticity in the outer
part of rat retina and whether GABAergic neurons being involved in following acute
high intraocular pressure (HIOP).Methods The intraocular pressure of all left eyes
was increased until b wave of flash electroretinogragh (fERG) disappeared and such
pressure maintained for 60 min. All right eyes served as normal control. The SD rats
were sacrificed respectively on hour 2,6,12 or day1, 3, 7, 14 following operation.
Immunofluorescent staining and western blot were used to detect the expression of
SYN and GABA. Results In the outer part of retina, SYN was mainly located in the
outer plexiform layer (OPL) in normal condition. After HIOP 2h, 6h, 12h, 1d, 3d and
7d, SYN positive products were extended to the inner part of the outer nuclear layer
(ONL). The expression of SYN in the outer part of retina was similar to the normal
condition 14d after HIOP. GABA was mainly located in the inner nuclear layer (INL)
and the inner part of OPL in normal condition. After HIOP 2h, 6h, 12h, 1d, 3d and
7d,GABA expression in OPL was downregulated, while upregulated in INL. On the day
th
7 , there was silkiness positive product in ONL, which was not double-staining with
th
SYN. Till the 14 day after HIOP, the positive product was decreased. Conclusion
There are synaptic plasticity in rat OPL and ONL following HIOP insult, GABA positive
neuron may not participate directly in this process.
Key words:
HIOP
retina
synaptophysin GABA