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Biology 120 Lab Exam 2 Review
Student Learning Services and Biology 120 Peer Mentors
Sunday, March 26th, 2017
6:00 pm
Arts 263
Important note: This review was written by your Biology Peer Mentors (not the lab instructors)!
It is designed to help you test yourself on topics and concepts covered in lab and should not in
any way be considered a “preview” of the actual exam.
You are encouraged to attempt to complete this review without the lab
manual, text book, your notes, or the internet. The peer mentors are
available while you are working to help you with any questions that
cause you difficulties.
NOTE: This version of the review has been revised.
Revision(s):
 Question 7e) had a repeated statement removed.
 Question 17 has had an answer changed for clarification
 The gel in question 18 has been changed to give a clear answer
and better reflect lab expectations.
Structured Study Session information
Mondays 2:00-3:20pm – Room 104 in the Arts Building (Mars)
Tuesdays 8:30am-9:50am – Room 213 in the Arts Library (Dakoda)
Tuesdays 5:30-6:50pm – Room G3 in the Murray Library (Maryam)
Wednesdays 11:30am-12:50pm – Room 211 in the Arts Building (Alexa)
Thursdays 11:30am-12:50pm – Room 124 in the Biology Building (Hayley)
Thursdays 3:00-4:20pm – Room 104 in the Arts Building (Tara)
Spot Test
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Short Answer
1. Fill in the Blanks:
a) Plants have a complex life cycle called an ___________________ ______
____________________, meaning that they have distinct multicellular haploid
and diploid stages.
b) _______________________ undergo meiosis to form haploid cells called spores.
c) Crossing over occurs in _________________ stage of meiosis.
d) In the fern life cycle the species is haploid during the __________________
generation, whereas the species is diploid during the ___________________
generation.
Questions and answers for the lab exam review will be posted after the session at: http://library.usask.ca/sss
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e) In animals meiosis only occurs in the ___________ or ___________ , and results
in the production of haploid gametes ( ___________ or ____________)
2. Harry Potter is in Herbology class and is studying mandrakes. A grumpy looking (R)
mandrake is dominant to happy looking (r) mandrakes. The mandrakes in his care, which
are all offspring of the same cross, are 50% homozygous dominant and 50%
heterozygous. What were the genotypes of the parents of these mandrakes?
3. During Potions, Harry learns that about 90% of wizards and witches perceive a sweet
taste from the Felix Felicis potion. The ability to taste this results from a dominant allele
(T). He also learns that in very rare cases drinking the potion causes people to turn
purple, the ability being called “purple-turners”. Being a purple-turner is a single locus
trait that is recessive (a) to normal pigment. A normally pigmented woman who cannot
taste the sweetness of Felix Felicis has a father who is a purple-turner non-taster. She
marries a homozygous, normally pigmented man who is a taster but who has a mother
that does not taste sweetness.
a) List all possible genotypes resulting from this cross.
b) What proportion of the children will be purple-turners? ______
c) What proportion of the children will be non-tasters? ________
4. During the class Care of Magical Creature, Hagrid is showing off Hippogriffs. The genes
that code for Hippogriff fur colour and nail sharpness are linked, 23.4 mu apart.
Brown fur (B) is dominant to white (b) and sharp nails (G) are dominant to dull (g).
Buckbeak’s mother had white fur and sharp nails, while his father had brown fur and
dull nails.
a) If Buckbeak who was brown fur and sharp nails mates with Fleetwing who has
white fur and dull nails what will be the phenotypic and genotypic frequencies of
their offspring?
b) Draw a cell from Buckbeak in Telophase II of Meiosis. Assume no crossing over
has occurred.
Questions and answers for the lab exam review will be posted after the session at: http://library.usask.ca/sss
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Questions and answers for the lab exam review will be posted after the session at: http://library.usask.ca/sss
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5. Squibness in wizarding families results in a complete lack of magical ability. It is caused
by the recessive allele (t) of an X-linked gene. Amanda, a carrier female for squibness
marries Jason, a normally magical male.
a) Draw a Punnet square for this cross.
b) If they have 2 sons and 4 daughters:
i. How many of the male offspring can be expected to be Squibs? ________
ii.
How many of the offspring can be expected to have normal magical
abilities? ________
iii.
How many of the female offspring can be expected to be carriers? ______
iv.
How many of the daughters can be expected to be magical? _______
6. In kneazles, magical creatures that resemble cats, the allele for black fur (B) is dominant
to the allele for beige fur (b), and the allele for a puffy-ended tail (T) is dominant to the
allele for a pointed-ended tail (t). The gene for fur colour is 9.6 map units from the gene
for tail type.
a) Draw a Punnett square to illustrate the cross between Mrs Paws, a kneazle with
beige fur and a pointed-ended tail and Crookshanks, a kneazle who had black fur
and a puffy-ended tail (both of Crookshank’s parents were homozygous for both
traits; his mom had black fur and a puffy-ended tail, and his dad had beige fur
with a pointed-ended tail).
b) What is the expected genotypic ratio of their offspring?
7. Instead of studying for his upcoming final exams, Harry Potter procrastinates and
instead studies house elf genetics (“elfnetics”) as they prepare Easter dinner at the
Hogwarts kitchen. In particular, he observes height and ear length, traits dependent on
genes that he knows are autosomal. In the P generation, the female house elf is short
and has short ears and the male house elf is tall with long ears. All of the house elves in
the F1 generation are short and have long ears. Use “A” to indicate height, and “B” for
ear length.
a) The genotypes of the house-elves in the P generation are:
Female: __________
Male: __________
b) The genotype of the house-elves in the F1 generation is: _________
c) An F1 female crosses with a true-breeding tall, short ears male.
This male’s genotype is: ______________
Questions and answers for the lab exam review will be posted after the session at: http://library.usask.ca/sss
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d) Harry Potter counts 16 offspring in the F2 generation (from the parents of
question c). List the number, phenotypes, and genotypes he would expect if
height and ear length were linked and no recombination occurred.
e) When he counts the F2 generation in the Hogwarts kitchen, he found the
following results:
1 short, long eared
7 tall, long eared
7 short, short eared
1 tall, short eared
Calculate the distance, in map units, between the genes for height and ear length.
8. The following pedigree illustrates the inheritance of Spontaneous Expelliarmus in a
population.
Use ‘E’ to represent the gene responsible.
A
Normal female
Normal male
B
C
Female with trait
D
a) What is most likely the mode of inheritance?
b) What is the genotype of A? _____________
c) What is the genotype of D? _____________
d) If individual B and C have a son what is the chance that he will have spontaneous
expelliarmus?
Questions and answers for the lab exam review will be posted after the session at: http://library.usask.ca/sss
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9. The following pedigree illustrates the inheritance of pterydactyl (development of bat
wings) in cats.
Use ‘P’ to represent the gene responsible.
Normal female
Normal male
Female with trait
Male with trait
a) What is the most likely mode of inheritance for this trait? _________________
b) Give the genotypes of the following individuals. Use the letter “P” for this trait.
6 _____________
c)
7 ______________
8 ______________
If Individuals 6 and 7 have another daughter, what are the chances that she will
be affected? ______
You are working as a forensic scientist for the Biology 120 Crime Scene Unit. You are called
to a crime scene in the biology building. At the crime scene, you discover blood splatter on
the mirror, hair fibres located in the sink, and urine samples along the wall.
10. What is the first step after you have collected cells from the scene?
11.
a) What component of blood, urine and hair fibres can you collect DNA from?
b) Which of the three (blood, urine, or hair fibres) would be the best sample
choice? Explain.
12. After extracting the DNA from your sample, you put it the thermal cycler. What other
ingredients should you add?
13.
a) Name the process that utilizes a thermal cycler.
b) What are the steps in this process?
Questions and answers for the lab exam review will be posted after the session at: http://library.usask.ca/sss
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14. What must you add to a DNA sample in the first step of Restriction Fragment Analysis?
15. Your sample is prepared, it is now time to analyze the DNA sample. Name the device
that you will use to load the samples into the wells of a gel.
16. What makes up the gel used in gel electrophoresis?
17. Which of the following combinations work for analyzing DNA samples with a gel?
a) Infrared light and Fast Blast
b) White Light and Methylene Blue
c) UV light and Methylene Blue
d) UV Light and Ethidium Bromide
18. You have successfully loaded your sample and run the gel. Based on the results below,
who is the murderer?
Ladder Crime Scene Victim
Suspect 1 Suspect 2
19. Name three uses for modern biotechnology.
i. ______________________________________________
ii. ______________________________________________
iii. ______________________________________________
20. What is recombinant DNA?
Questions and answers for the lab exam review will be posted after the session at: http://library.usask.ca/sss
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