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Introduction to Fast Plants Development Dr. Paul H. Williams at the University of Wisconsin-Madison developed fast plants. Originally developed for research in disease resistance. Extended the material for classroom use. Williams PH and C Hill. 1986. Rapid-cycling populations of Brassicas. Science 232: 1385-1389. More than 150 traits identified in the material. Original population was Brassica rapa. Also developed populations for six related species. Seeds can be stored up to 10 years. Fast Plants Rapid-cycling Brassicas Crucifers Cabbage Turnips Broccoli Cauliflower Mustards Canola Characteristics of Seed Plants Seed plants outnumber seedless plants by more than one to ten! All seed plants share two characteristics: ♦ They have vascular tissue ♦ They use seeds to reproduce Vascular Tissue Most seed plants live on land. This causes challenges for the plants. ♦ How can they stand up right? • The thick walls of the vascular tissue help to support the plant ♦ Supply their cells with water, food, & nutrients? • Phloem is what the food moves through • Xylem is how water moves from roots to plant Seeds are structures that contain a young plant inside a protective covering. ♦ Three important parts: • Embryo – zygote or fertilized egg • Stored food – cotyledons or leaves that store food • Seed coat – skin or protective covering Types of Fast Plants Standard Purple Stem, Hairy Non-Purple Stem Yellow-Green Leaf Non-Purple Stem, Yellow-Green Leaf Rosette dwarf (Gibberellin deficient) Tall Plant (Gibberellin Overexpression) Petite Plant (Gibberellin deficient) Variegated Life Cycle of Brassica rapa budding germination mature harvesting 1. Requirements for Growing There must be 24-hour lighting from cool fluorescent bulbs The light must be 5 – 10 centimeters above the tallest plant 2. Requirements for Growing There must be a constant water source Your plants will rest on top of a self-watering system like this one. 3. Requirements for Growing Room temperature Between 65° and 78° F 4. Requirements for Growing Consistent source of nutrition • 2 – 3 Fertilizer pellets per planting cell Planting Materials Formulated soil Pelletized fertilizer Quad Wicks Anti-algal squares Labels Watering mat & watering system Directions for Growing Your group will plant one quad, which consists of four smaller cells Push the wicks into each cell, so that they extend through the bottom of the quad by about one centimeter Directions for Growing Fill each cell half full with moist potting soil Add 1-3 fertilizer pellets to each cell. Add more soil to fill each cell. Do not pack the soil! Use your finger to make a 4 mm depression in the soil. Drop 1-2 seeds into the soil of each cell. Cover seeds with enough soil so the seeds are not visible. Directions for Growing Label your quad with one pot label. Water gently with pipette until water drips from each wick. Place the quad on the water mat. Water with the pipette for the first three days to ensure adequate moisture for germination. Radicle Monday (Day 0): Your seeds Tuesday and Wednesday (Days 1 & 2): Seed Germination Cotyledon Root Root hairs Hypocotyl Thursday and Friday (Days 3 & 4): Your seedling Thursday and Friday (Days 3 & 4): Your seedling Over the weekend (Days 4 through 6) The hypocotyls will extend as the plant seeks light True leaves Monday (Day 7): The developing plant Shoot Meristem Nodes (where the leaves are attached) Internode Stamens Petals Week 3 (Days 14-20): The flower is ready to pollinate Pistil Sepals Petal Come in all shapes, sizes, and colors The colorful structures that you see when a flower opens Not all plants have these Their colors and shapes attract insects and other animals to the plant This ensures that pollination will occur Sepal Leaf-like structures They protect the developing flower Pistil: Ovary, Ovules, & Stigma A protective “vessel” where seeds develop These are located within the flower When plants reproduce through seeds they are called angiosperms Some flowers have more than one pistil The sticky tip of the pistil The purpose is collect pollen Stamen The filament is the stem of the male reproductive part The anther is where the pollen is produced First, we need to know the parts of the plants and their functions Click on each part label to learn more about it … Back Pollinating with Bees Thorax of bee Where is the pollen on the plant? The stamens Where does it need to be deposited? The pistil Fertilization has occurred Developing seeds Developing Fruit Harvesting your seeds Moist Paper Towel Evenly scatter seeds on paper towel Where should these seeds be kept? A cool, dark place Gallery of Fast Plant Images http://www.fastplants.org/ac tivities.gallery.php#menu Fast Plants Website www.fastplants.org Page 3 O2 CO2 Leaves contain many pores (called stomata) Page 3 Chloroplasts contain a green pigment called chlorophyll Page 3 Glucose! Homework – Drawing Dicots Use the information in the left and center columns to help you draw a picture in the right column that represents characteristics of dicots. Monocot Seeds Roots Stems Leaves Flowers Examples Dicot Monocot Dicot Seeds One cotyledon Stays below ground Two cotyledons Come out of ground w/ young plant Roots Fibrous roots that spread wide Single, long, taproot system Stems Vein bundles are scattered randomly Vein bundles arranged in circular pattern Leaves Parallel veins Branched veins Flowers Multiples of 3 Multiples of 2, 4, or 5 Examples Grasses; corn, rye Grains; wheat, rice Lilies Beans, peanuts, peas, broccoli, apples Roses Page 1 Growth Requirements Continuous fluorescent light Continous Water (Tray with water that each pot sets in) Continous Fertilizer (Pellets) Peat/Vermiculite Potting Mix Monitoring the Growth of your Fast Plants