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FEMS Pathogens and Disease, 74, 2016, ftv125 doi: 10.1093/femspd/ftv125 Advance Access Publication Date: 27 December 2015 Short Communication SHORT COMMUNICATION Plasma is the main regulator of Staphylococcus epidermidis biofilms virulence genes transcription in human blood Angela França and Nuno Cerca∗ CEB – Centre of Biological Engineering, LIBRO – Laboratory of Research in Biofilms Rosário Oliveira, University of Minho, Campus de Gualtar, 4710-057 Braga, Portugal ∗ Corresponding author: Centro de Engenharia Biológica, Universidade do Minho, Campus de Gualtar, 4710-057 Braga, Portugal. Tel: +351-253-60443; Fax: +351-253-678-986; E-mail: [email protected] One sentence summary: Plasma is the component of human blood that regulates the transcription of several genes involved in Staphylococcus epidermidis biofilms virulence. Editor: Thomas Bjarnsholt ABSTRACT Staphylococcus epidermidis is frequently associated with the emergence of medical-device-associated bloodstream infections, due to its ability to form biofilms on the surface of vascular catheters. Although these biofilms may be in continuous contact with human blood, how S. epidermidis biofilm cells interact with blood and its cellular and soluble components is poorly understood. Herein, we evaluated biofilm structure, biofilm cells culturability and viability, and the transcription of a panel of genes associated with S. epidermidis biofilms virulence, upon interaction with whole human blood or plasma. Our results showed that although whole human blood caused significant alterations in biofilm structure and in the number of culturable and viable cells, plasma was the main regulator of the transcription of genes with central role in biofilm formation, maturation and immune evasion. These findings highlight the urgent need to intensify studies aiming to evaluate the impact of host soluble factors on S. epidermidis biofilms fitness and persistence. Keywords: Staphylococcus epidermidis; biofilms; human blood; plasma; virulence genes; gene expression INTRODUCTION Biofilms present a significant challenge to current clinical practice due to their increased tolerance to antibiotics and host immune response being, consequently, commonly associated with the development of chronic and recalcitrant infections (Costerton, Stewart and Greenberg 1999). Staphylococcus epidermidis, a skin- and mucosae-colonizing bacterium, has a remarkable ability to form biofilms on the surface of indwelling medical devices (Vadyvaloo and Otto 2005), particularly on peripheral and central vascular catheters. In fact, it was reported that 22% of bloodstream infections diagnosed among patients in intensive care units, in the USA, are due to S. epidermidis biofilm for- mation on these catheters (Otto 2009). Nevertheless, although medical-device-related bloodstream infections are one of the most frequent infections caused by S. epidermidis biofilms, the interaction between biofilm cells and human blood is not well understood. Recently, we have shown that the incubation with human blood led to an extensive reprogramming of S. epidermidis biofilm cells transcriptome (Franca et al. 2014). Nevertheless, since human blood is a complex mixture of cells and soluble molecules, both involved in bacteria targeting, it was not possible to identify which of these fractions were causing the transcriptional alterations observed in that study. Therefore, we became interested in differentiating the response of S. epidermidis Received: 23 September 2015; Accepted: 22 December 2015 C FEMS 2015. All rights reserved. For permissions, please e-mail: [email protected] 1 2 FEMS Pathogens and Disease, 2016, Vol. 74, No. 2 biofilms to blood circulating cells versus soluble factors. Hence, herein, S. epidermidis biofilms were incubated, respectively, with whole human blood or human plasma and the abundance of transcripts encoding S. epidermidis biofilm virulence factors was assessed. In addition, biofilm structure and biofilm cells culturability and viability were also evaluated. R lithium Human blood was collected, into BD Vacutainer heparin tubes (NJ, USA), from healthy adults volunteers and under a protocol approved by the Institutional Review Board of the University of Minho (SECVS 002/2014), which is in strict accordance with the Declaration of Helsinki and Oviedo Convention. Informed written consent was signed by all volunteers. Plasma was obtained by centrifugation of whole blood at 300 g, for 20 minutes, at 4◦ C. Biofilms of S. epidermidis strain RP62A (ATCC 35984) were formed, in 24-well plates (Orange Scientific, Braine-l’Alleud, Belgium), by adding 1.6 μL of overnight culture into 1 mL of tryptic soy broth (Liofilchem, Teramo, Italy) supplemented with 1% glucose (v/v) and by incubating at 37◦ C in an orbital shaker at 120 rpm. Twenty-four hours-old biofilms were incubated for 2 hours, under static conditions, at 37◦ C and 5% CO2 with either 1 mL of whole blood or plasma. Biofilms were then washed twice with 0.9% of NaCl and immediately suspended in 1 mL of the same solution, for the evaluation of biofilms biomass and biofilm cells culturability and viability, or in 1 mL RNA Protect bacteria reagent (QIAGEN, Hilden, Germany), for the quantification of gene expression levels. Biofilms biomass was determined by optical density (OD) (Freitas et al. 2014) at 640 nm, the number of culturable cells by the standard CFU counting method, and the number of viable cells by flow cytometry (EC800 flow cytometer, Sony, CA, USA) using SYBR Green and propidium iodide staining as described before (Cerca et al. 2011b). All the quantification analyses were performed using blood/plasma of four different donors (two males and two females), with triplicates for each condition. For confocal laser scanning microscopy (CLSM), 24 hours-old biofilms were formed on Nunc Thermanox coverslips (Thermo Scientific, MA, USA), which were placed inside the wells of the 24-well plates used. After incubation with blood or plasma, the biofilms formed on the coverslips were washed twice with ultrapure water, the coverslips removed from the well and placed onto a glass slide. Then, polysaccharides were stained with Wheat Germ Agglutinin (WGA) Oregon Green 488 (Life Technologies, CA, USA) and nucleic acids with DAPI (Sigma, MO, USA), as detailed elsewhere (Cerca et al. 2012). Biofilm images were acquired using an Olympus FluoView FV1000 (Lisboa, Portugal) microscope. Biofilms were observed using a ×60 water-immersion objective (60 ×/ 1.2 W). Images were taken, with 1024 × 1024 resolution, in at least four different regions of each of the surfaces analyzed. This experiment was performed using blood/plasma of three different donors (two males and one female), with duplicates for each condition. For the quantification of gene expression, the abundance of target transcripts was determined as optimized before (Franca et al. 2014). In brief, total RNA was isolated from biofilms incubated with blood/plasma of five different donors (three males and two females) by combining silica beads lysis with RNeasy mini kit (QIAGEN purification column system. Genomic DNA was degraded by DNase I (Thermo Scientific) and complementary (c) DNA synthesized, from 300 ng of total RNA, by RevertAid H minus Reverse Transcriptase enzyme (Thermo Scientific) in the presence of random primers (NZYTech, Lisboa, Portugal), and in a 10 μL reaction volume. Finally, qPCR run was performed by mixing 2 μL of 1:400 diluted cDNA with 5 μL of Q R Green Supermix (Bio-Rad, CA, USA), 0.5 μL of each forSYBR ward and reverse primers at 10 μM and 2 μL of nuclease-free water. The run was completed in a CFX96TM (Bio-Rad) thermal cycler with the following parameters: 94◦ C 10 minutes and 35 cycles of 94◦ C 15 seconds, 20 seconds at 58◦ C and 72◦ C for 25 seconds. The abundance of transcripts was determined by applying the Pfaffl equation (Pfaffl 2004) using 16 rRNA as reference gene. The sequence of the primers used can be found in previous publications (Franca et al. 2012; Sousa, Franca and Cerca 2014), with the exception of the primers used to detect sepA (FW: TCTTAAGGCATCTCCGCCTA and RV: GTCTGGTGCGAATGATGTTG, 194 bp amplicon) and capA (FW: TGATTATGCGAACCTTTCCAG and RV: TAGCGAATGCTGGTGCAG, 175 bp amplicon) transcripts. The sequences of target genes were downloaded, for primer design, using GeneBank accession number NC 002976. The heat map of relative transcription was created using matrix2png interface (Pavlidis and Noble 2003). Statistical analysis was carried out using GraphPad Prism (CA, USA). Kruskal–Wallis and Dunn’s multiple comparison tests were applied. Accordingly, data are depicted in median with range. Differences among groups were considered significant when P was less than 0.05. As expected, biofilms biomass (Fig. 1A) and biofilm cells culturability (Fig. 1B) and viability (Fig. 1C) were significantly reduced in the presence of whole human blood. A more pronounced reduction, however, was observed in the culturability of biofilm cells rather than in viability. This difference was not surprising since biofilms are highly heterogeneous communities comprising cells in different metabolic states including, among others, dormant cells (Stewart and Franklin 2008). In addition to the intrinsic biofilm heterogeneity, the high concentration of glucose (1%) used for the growth of these biofilms is known to further stimulate dormancy within S. epidermidis biofilms (Cerca et al. 2011a). By comparing the number of CFU and the number of viable cells obtained, it was possible to determine the proportion of dormant cells in each condition. Interestingly, although control biofilms already presented 0.5 Log10 of dormant cells, the incubation with either blood or plasma led, respectively, to an increase of 2-fold (1 Log10 ) and 1.5-fold (0.75 Log10 ) in the proportion of dormant cells. This phenomenon may contribute to the persistence of biofilm cells in the host as it was recently shown that S. epidermidis biofilms with higher proportion of dormant cells have lower inflammatory profile (Cerca et al. 2014). To further characterize the influence of human blood and plasma on S. epidermidis biofilms, its structure was analyzed by CSLM upon interaction with whole human blood or plasma. In agreement with the previous results obtained, the incubation with whole human blood resulted in a significant reduction (P ≤ 0.001) of the maximum biofilm thickness, from 35.6 ± 13.3 μm (control) (Fig. 1D) to 8.2 ± 4.3 μm (blood-exposed biofilms) (Fig. 1E). Human plasma-exposed biofilms did not present any significant alteration in either biofilm structure or thickness (22.1 ± 4.5 μm), when compared with the control (Fig. 1F). Finally, to investigate which of blood components, cells or soluble factors, were in fact influencing S. epidermidis biofilms gene expression profile, the transcription levels of genes with a central role in biofilm development/maturation such as aap (Hussain et al. 1997; Rohde et al. 2005), bhp (Tormo et al. 2005), icaA (Mack et al. 1996) and lrgB (Bayles 2007) and in immune evasion such as icaA (Cerca et al. 2006; Kristian et al. 2008), capA (Kocianova et al. 2005), mprF/fmtC (Gill et al. 2005; Richards et al. 2015) and sepA (Lai et al. 2007) were measured. Despite the characteristic variability inherent to each donor, the transcription of these genes was significantly increased, when compared with biofilm cells harvested before any manipulation, in biofilms exposed to either whole human blood or plasma (Fig. 1G). However, when comparing the abundance of transcripts between França and Cerca 3 Figure 1. The influence of whole human blood and plasma on S. epidermidis biofilms. (A) Biofilms biomass, (B) number of CFU or (C) viable cells after incubation with whole human blood or plasma. The bars represent the median with range of four independent assays. (D, E, F) Representative images of the three-dimensional structure of control biofilms (D), biofilms exposed to blood (E) or plasma (F). DAPI, which stains nucleic acids, is represented in red, and WGA, which stains polysaccharides, is represented in green. (G) Heat map of the relative transcription levels (comparing to T0h) of the selected virulence genes upon 2 hours of interaction with whole human blood or plasma. (H) Relative transcription levels of the selected virulence genes in whole human blood (comparing to plasma). The vertical bars represent the median of 5 independent assays. Log2 fold change equal to 0 indicates no alteration in the transcription, Log2 fold change below 0 indicates increase in transcription in plasma-exposed biofilms and Log2 fold change above 0 indicates increase in transcription in blood-exposed biofilms. Each symbol represents a single donor. Statistical significance among groups was determined using Kruskal–Wallis and Dunn’s multiple comparison tests. ∗∗ P ≤ 0.01, ∗∗∗ P ≤ 0.001. blood- and plasma-exposed biofilms, no meaningful differences were observed with the exception of sepA and aap transcripts, which were found in higher amount within biofilm cells incubated with human plasma (Fig. 1H). SepA is a protease that is involved in bacterial immune evasion through the degradation of antimicrobial peptides produced by the host (Lai et al. 2007). As it could be expected, as a defense mechanism, the transcription of sepA would be increased in the presence of either blood or plasma. However, the increased transcription observed in plasma-exposed biofilms may be related to the overrepresentation of host proteins and due to the absence of leukocytes, which may interact with biofilm cells and selectively 4 FEMS Pathogens and Disease, 2016, Vol. 74, No. 2 trim the transcription of particular genes. Similarly, the higher levels of aap transcripts detected in plasma-exposed biofilms were probably due to the same reasons. Interestingly, it was shown that host proteases are capable of activating Aap by proteolysis, which in turn will help to establish a more cohesive biofilm with increased resistance to host factors (Rohde et al. 2005). The ability of S. epidermidis biofilm cells to evade bacteriatargeting molecules present in human plasma provides an exceptional competence to survive in a wide variety of human fluids. Apart from constituting approximately 55% of whole human blood, plasma is present, at varying concentrations, in several other important body fluids such as lymphatic and synovial fluids, acute soft tissue wounds and burn wounds exudates (Cardile et al. 2014), fluids where S. epidermidis biofilm infections may have special incidence. Overall these results indicate that, although plasma is less capable of reducing the number of biofilm cells or biomass, is the main regulator of the transcription of genes encoding key biofilm virulence factors (Fig. 1G and H). In addition, this finding highlights the urgent need to study, in more detail, the interaction between the components of human plasma and S. epidermidis biofilms in order to better understand the capacity of these bacterial cells to persist in the host. It is however important to highlight that the proteins that are secreted during bacterial infections (acute-phase proteins, such as C-reactive protein), which may polarize biofilm response (Malachowa et al. 2011), are not present in healthy individuals such the ones used for the experiments herein reported. Hence, in the future, to better comprehend the interplay between S. epidermidis biofilm cells and human plasma components, it would be important to repeat these experiments using plasma of infected patients. In addition, other S. epidermidis strains need to be tested since strainto-strain variability may occur, as we have previously shown (Franca et al. 2013). ACKNOWLEDGEMENTS NC is an Investigador FCT. The authors would like to thank Sandra Maia and Filomena Costa for human blood collection procedure. The authors declare no conflict of interest. FUNDING This work was supported by European Union funds (FEDER/COMPETE) and by national funds (FCT) under the project with reference FCOMP-01-0124-FEDER-041246 (EXPL/BIAMIC/0101/2013). The authors thank the FCT Strategic Project of UID/BIO/04469/2013 unit and the project FCOMP-01-0124FEDER-027462 (RECI/BBB-EBI/0179/2012). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. AF acknowledges the financial support of an individual grant SFRH/BPD/99961/2014. Conflict of interest. None declared. REFERENCES Bayles KW. The biological role of death and lysis in biofilm development. Nat Rev Microbiol 2007;5:721–6. Cardile AP, Sanchez CJ, Jr, Samberg ME et al. Human plasma enhances the expression of Staphylococcal microbial surface components recognizing adhesive matrix molecules promoting biofilm formation and increases antimicrobial tolerance in vitro. BMC Res Notes 2014;7:457. Cerca F, Andrade F, Franca A et al. 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