Download 14-3-3 Sigma (S7323) - Datasheet - Sigma

14-3-3 Sigma
human, recombinant
expressed in Escherichia coli
histidine-tagged (C-terminal)
Catalog Number S7323
Storage Temperature –20 °C
Synonyms: 14-3-3σ, Stratifin, Epithelial cell marker
protein 1, HME1, SFN
Product Description
The 14-3-3 proteins are a multifunctional protein family
composed of seven mammalian isoforms, which
interact with over 200 different intracellular molecules
including kinases, phosphatases, transcription factors,
scaffold proteins, and DNA. 14-3-3 proteins are found in
all eukaryotic cells involved in a multitude of biological
processes and play a regulatory role in processes such
as apoptotic cell death, mitogenic signal transduction,
and cell cycle control. In human cells, this family of
highly conserved proteins consists of seven distinct
gene products of ∼30 kDa (β, γ, ε, η, σ, ζ, and τ).1
14-3-3σ is a member of the 14-3-3 family of proteins
that play a critical role in signal transduction pathways
and cell cycle regulation. Among the family members,
14-3-3σ is unique. It is the only 14-3-3 isoform induced
by the tumor suppressor protein p53, in response to
γ-irradiation and other DNA-damaging agents.2 It is a
p53-regulated inhibitor of G2/M progression.3 14-3-3σ
acts as a tumor suppressor gene that is inactivated by
methylation of its 5′-CpG islands in epithelial tumor
cells. In normal epithelia, 14-3-3σ is commonly
unmethylated. 4
The product is supplied in a 0.2 µm filtered solution of
50 mM Tris-HCl, pH 7.6, with 1 mM EDTA, 1 mM DTT,
and 20% (w/v) glycerol.
Purity: ≥90% (SDS-PAGE)
Precautions and Disclaimer
This product is for R&D use only, not for drug,
household, or other uses. Please consult the Material
Safety Data Sheet for information regarding hazards
and safe handling practices.
Storage/Stability
The product ships on dry ice and storage at –20 °C is
recommended. Upon first use, it is recommended to
divide the enzyme into aliquots and store at –20 °C.
The product is stable at –20 °C for at least 2 years.
References
1. Wilker, E.W., et al., A structural basis for 14-3-3-σ
functional specificity. J. Biol. Chem., 280, 1889118898 (2005).
2. Yang, H., et al., DNA damage-induced protein
14-3-3σ inhibits protein kinase B/AKT activation
and suppresses AKT-activated cancer. Cancer
Res., 66, 3096-3105 (2006).
3. Hermeking, H., et al., 14-3-3 sigma is a
p53-regulated inhibitor of G2/M progression. Mol.
Cell, 1, 3-11 (1997).
4. Kishor, B., et al., The tumor suppressor gene
14-3-3 is commonly methylated in normal and
malignant lymphoid cells. Cancer Epidemiol.
Biomarkers Prevent., 12, 165-169 (2003).
TD,KAA,EB,MAM 01/09-1
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