Download ISTANBUL MEDIPOL UNIVERSITY Course Learning Outcomes of

ISTANBUL MEDIPOL UNIVERSITY
Course Learning Outcomes of MICROBIOLOGY
Course Code and Title
DHF1295, TF1295 - MICROBIOLOGY
Department /
Programme
School of Medicine
Language of Education English
Course Type
Yes
Lecturer(s)
Prof. Dr. Nuri KİRAZ
Office Hours
Every Friday 09:00 - 12:00
Course Objective
The aim of this course is to mention the importance of microorganisms and microbiology, to teach prokaryotic
cell biology (metabolism and genetic), define nutrient and other physical needs of microorganisms, to teach basic
laboratory techniques of working with microorganisms (culture techniques, Gram stain, Acid fast stain, spore
stain) with practical sessions in laboratory.
1.Describe Scope and History of Microbiology
1.1.Define microorganism, bacteriology, mycology, parasitology, immunology, virology, microbial genetics
1.2. Identify groups of organisms and infectious agents studied in microbiology and list their general
characteristics
1.3.State the contributions to microbiology made by Hooke, van Leeuwenhoek, Pasteur, Redi
1.4.Discuss the significance of the following in contributing to our understanding of disease today: germ theory,
cell theory, spontaneous generation, biogenesis
2.An Introduction to Taxonomy: The Bacteria
2.1.Discuss how and why microorganisms are named.
2.2.Describe the five kingdom classification system, and place organisms in the correct kingdom based on their
cell type (prokaryotic or eukaryotic), number of cells (unicellular or multicelluar), and method of obtaining food
(autotroph or heterotroph, absorption or ingestion)
2.3.Explain the method of classification of organisms from kingdom to species
2.4.Recognize scientific names and know the correct way to write them
3.Microscopy and Staining
3.1.Know the parts of a light microscope and their functions.
3.2.Explain the terms magnification, resolution, refractive index
3.3.Calculate total magnification.
3.4.Know the difference between simple, basic, acidic, differential and special stains.
3.5.Describe how to perform a Gram stain.
3.6.Describe the appearance of Gram positive and Gram negative cells after each step of the Gram stain.
4.Characteristics of Prokaryotic and Eukaryotic Cells
4.1.Explain what features of a cell are distinguished in the following stains: Gram stain, acid fast, endospore,
flagella, capsule.
4.2.Describe the morphology (shape and arrangement) of microorganisms based on terms given in class.
4.3.Compare and contrast the overall cell structure of prokaryotes and eukaryotes.
4.4.Identify the three basic shapes of bacteria.
4.5.Describe the structure and function of the glycocalyx, flagella, axial filaments, fimbriae and pili.
4.6.Compare and contrast the cell walls of Gram positive bacteria, Gram negative bacteria and mycoplasma.
4.7.Describe the structure, chemistry and function of the prokaryotic plasma membrane.
4.8.Define simple diffusion, osmosis, facilitated diffusion, active transport and group translocation, exocytosis,
endocytosis.
4.9.Identify the functions of the nuclear area, ribosomes and storage granules.
Learning Outcomes
and Subordinate Skills
5.Growth and Culturing of Bacteria
5.1.Define growth as it pertains to bacteria.
5.2.Explain the process of binary fission.
5.3.Identify the parts of a growth curve and explain what is occurring in each phase
5.4.Define aseptic technique, and demonstrate with applications.
6.Essential Concepts of Metabolism
6.1.Define metabolism, and distinguish between anabolism and catabolism.
6.2.Explain the overall function of metabolic pathways.
6.3.Identify common carriers of hydrogen, electrons and energy within the cell.
6.4.Compare aerobic and anaerobic respiration and fermentation in terms of products produced (final electron
acceptors), pathways employed, and amount of useful energy produced.
6.5.Describe the significance of glycolysis and the citric acid cycle in relation to overall metabolism (not just the
breakdown of glucose).
6.6.Calculate the total numbers of ATP molecules produced by aerobic respiration in prokaryotes and eukaryotes.
6.7.Define the terms phototroph, chemotroph, autotroph, heterotroph.
7.Microbial Genetics
7.1.Define genetics, genotype and phenotype.
7.2.Identify the structure of DNA in prokaryotes and eukaryotes.
7.2.Identify the structure of DNA in prokaryotes and eukaryotes.
7.3.Explain the meaning of the following terms as they pertain to DNA structure: complementary, antiparallel,
sugar phosphate backbone, semiconservative replication, hydrogen bonds.
7.4.Distinguish between DNA replication, transcription, translation and reverse transcription.
7.5.Explain the difference between leading strand and lagging strand DNA synthesis.
7.6.Define the central dogma of molecular biology.
7.7.Describe the function of each of the three major classes of RNA molecules.
7.8.Compare and contrast the structure of RNA and DNA.
7.9.Define spontaneous and induced mutations and compare missense, nonsense and frameshift mutations.
8.Viruses
8.1.Describe the components of a virus.
8.2.Explain what characteristics are used to classify viruses.
8.3.Define viroid and prion.
8.4.Explain what is meant by latent and slow viral infections.
9.Eukaryotic Microorganisms (Fungi) and Parasites
9.1.Define the terms parasite and parasitology.
9.2.Explain the method of classifying organisms in the kingdoms Protista and Fungi.
9.3.Define hyphae, mycelium.
9.4.Identify the two major groups of parasitic helminthes.
9.5.Distinguish between flatworms and roundworms.
Generic Competences
Creative, questioning, work in teams, critical thinking.
Outline
1.Introduction to microbiology
2.Classification of microorganisms
3.Introduction to bacteriology and bacterial classification
4.Orientation to the Microbiology Laboratory (Laboratory)
5.Safety Procedures and Precautions, General Laboratory Directions, The Microscope (Laboratory)
6.Bacterial Structure
7.Bacterial Metabolism
8.Bacterial Genetics, Bacteriophages and Extrachromosomal Structures
9.Gram Stain, Simple Stains (Laboratory)
10.Rickettsia, Chlamydia and Mycoplasmas
11.Introduction to Virology
12.Viral Replication and Genetics
13.Introduction to Mycology
14.Introduction to Parasitology
15.Disinfection Sterilization Antisepsis
Instructional Methods
and Techniques
Lecture, team/group work, experiment, drill and practice, brain storming, preparing reports.
Course Requirements
TF1092 DHF1092 Medical Biology and Genetics
Textbook Murray PR, Rosenthal KS, Pfaller MA. Medical microbiology. 6th ed. Philadelphia: Mosby/Elsevier; 2009.
References Suggested Tortora GJ, Funke BR, Case CL. Microbiology : an introduction. 9th ed. San Francisco: Pearson Benjamin
Sources
Cummings; 2007.
Hours