Genetics /Microbiology Final Exam Study Guide This study guide is to provide you with a listing of topics covered during the second half of the year in Genetics and Microbiology. It is important for you to know the details of these major concepts and to be able to relate their importance. As this is a listing of major concepts for your review it is not an all inclusive of every question on the final exam. Good practice would be to write down what you remember about each topic and then to review your notes, ppts, worksheets, study guides, labs, and textbook sections to clarify as needed. Behavioral Disorders What are behavioral traits? Techniques for studying behavioral traits IQ scores rangesGenetic and Environmental influences Behavioral Disorders- Overview/Definition/Symptoms Schizophrenia Narcolepsy Anorexia Bipolar Autism DNAScientists/ their discoveries andexperiments Structure of DNA- components Purines vs Pyrimidines Base pair rules Chargaff’s Rule DNA replication Process Enzymes involved Compare and contrast the different RNAs Introns vs Exons Protein Synthesis- steps Transcription Translation Biotechnology Restriction enzymes Plasmids Recombinant technology Gel Electrophoresis Process Methodology Interpretation of results Determination of genotypes Nucleotide sequence using a gel pattern Microbiology Structures of a microbial cell- Appendages and function Flagellar morphology- types Morphology Basic shapes and arrangements. ReproductionGram Stain Theory Differences between Gram positive and Gram negative based on cell wall differences Gram Stain Test Procedure Reagents and Results for Gram positve and Gram negative Bacterial Growth Requirements Temperature pH Gas Requirements Differences between aerobic and anaerobic bacteria What is a facultative anaerobe Bacterial Growth Curve-Draw and label 4 different phases and what occurs in each Which phase is best for antibiotic testing? Which phase is best for biochemical testing and gram staining? Metabolism What are oxidation and reduction reactions Aerobic Respiration- steps and ATPs produced in each Glycolysis Krebs Cycle Electron transport Final electron acceptor? What are NAD and FAD – what is their significance in production of ATP? Types of Fermentation Anaerobic Respiration Final electron acceptors? Role of Enzymes Structure- apoenzymes, holoenzymes, cofactors Factors that influence them Control Mechanisms Competitive Inhibition Feedback Host- Microbe Interactions Symbiotic relationships DiseasePathogensVirulenceDescribe Koch’s postulates Microbial Control- Physical and Chemical Control Resistance Levels Compare and Contrast : DisinfectionSterilizationSanitizationAutoclave use- UV radiation and effects on Bacillus spPasteurization- Anibiotic Disk Diffusion Test- Kirby-Bauer Procedure Standardization Interpretation of Results Quality Control Characteristics of an ideal Antimicrobial Drug How do antimicrobials attack bacterial cells? Immunology3 lines of defense Cellular vs humoral immunity Describe Inflammatory Response Passive and Active Immunity How does a vaccine work? Define an effective vaccine?