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4/15 Daily Catalyst Pg. 53 Gel Electrophoresis
 1. Why do insertions and deletion mutations have a
more devastating affect?
 2. Why do women need to be cautious around
potential mutagens?
 3. Determine the mutation:
 DNA: ACG- TAC-GGC
 Mut: AGG-ACG -GGT
4/15 Class Business Pg. 53 Gel Electrophoresis
 Quiz #12 DNA on Friday
 DNA test 4/21
 Study guide due test
 Review day on 4/20
 DNA Extraction lab on Thursday
 Be here! Labs are test grades!
 Interims come out this Friday
 Signed for extra credit
 Parent Night on Thursday 6-7:30
 Extra Credit
4/15 Agenda Pg. 53 Gel Electrophoresis
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Daily Catalyst
Class Business
Gel Electrophoresis notes
Project work time
Exit Ticket #9 Mutations
Homework: finish the gel electrophoresis packet and
DNA packet pg. 5
4/15 Objective
Genetic engineering techniques can
manipulate the heritable information
of DNA by gel electrophoresis.
Video Clip
 Gel Electrophoresis video clip
 https://www.youtube.com/watch?v=dkp12h31kH4
Genetic Engineering
 Key Point #1: Genetic engineering (GE)
 Scientific alteration of the DNA (genetic material)
 Transferring genes between organisms or modifying the
genes in an organism to remove or add a desired trait
 For example: Genetic engineering has been employed to create
bacteria that synthesize insulin and other human proteins.
Gel Electrophoresis
 Key Point #2: Gel Electrophoresis
 The separation of DNA based on
positive and negative charges
 What is DNA’s charge?
 DNA is negatively charged
 How?
 Negative phosphate groups
How Gel Electrophoresis works
 DNA molecules are added to a gel
 DNA molecules move down through the gel
 Key Point #3: Molecules move based on their
size
 Smaller molecules move faster through the gel
Why are we learning about this?
 Every living cell has DNA
 Every living cell has unique DNA
 Key Point #5: DNA is like our fingerprint, unique to us and can link
us to the scene of a crime
 Gel electrophoresis has only been used since 1980’s
 This technology has revolutionized the way crimes are solved.
How does this process work?
 DNA is collected from the crime scene and from
potential suspects
 DNA is dropped into individual columns
 The gel is connected to a power source (+ and -)
 The DNA moves down the gel
 Similar DNA will show the same patterns and the
patterns are matched to the suspect’s.
Example
 Crime video clip
 http://www.ksl.com/?sid=18133538
Project
 Directions: Today, you and a partner will read
several gels and determine if the suspect is
guilty or not. When you finish the gel project,
prepare for exit ticket.
 Time: 18 minutes
 Noise: 1 (with partner)
Exit Ticket #9 Mutations
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Name: ___________ Date: 4/15 Period: 1 Score ______/4
1. Define mutations.
2. Are all mutations bad?
3. Mutations can lead to an increase in ______________.
4. What causes mutations?
Exit Ticket #9 Mutations
 Name: ___________ Date: 4/15 Period: 1 Score ______/4
 1. Define mutations.
 A random change in DNA
 2. Are all mutations bad?
 NO, some are helpful
 3. Mutations can lead to an increase in ______________.
 Variation
 4. What causes mutations?
 Mutagens