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Transcript
Biology – Observing Mitosis Name _____________________________ Mrs. Douma Date: ________________ Mitosis in an Onion Root Introduction: Mitosis can be observed in cells that are in a state of growth. In this lab, you will observe cells and identify which stage of cell division the cells are in. To help you do this, let's review what characteristics to look for at the different stages. Also remember, Interphase is not technically a part of Mitosis, but it is part of the cell cycle and many of the cells you will be looking at are in Interphase - so it is included in this activity. What are the Distinguishing Characteristics of each stage? Interphase ________________________________________________________________________ ________________________________________________________________________ ________________________________________________________________________ Prophase ________________________________________________________________________ ________________________________________________________________________ ________________________________________________________________________ Biology – Observing Mitosis Mrs. Douma Metaphase ________________________________________________________________________ ________________________________________________________________________ ________________________________________________________________________ Anaphase ________________________________________________________________________ ________________________________________________________________________ ________________________________________________________________________ Telophase ________________________________________________________________________ ________________________________________________________________________ ________________________________________________________________________ Procedure: You will be given a slide of Allium, which is an onion root tip and also Whitefish blastula (zygotes). View the root tip under the microscope and search for organized blocks of cells where nuclei are plainly visible. (Most activity will be occurring at the tip of the root). Move the slide around until you find a good spot that shows the cells in various stages of mitosis. Draw an example of a cell in each of the five stages. Make sure you label all the structures. When you have finished, do the same with the slide of the Whitefish blastula. Plant Cell (Allium) Stage ........................................ Estimated Number of Cells in this Stage …….. Stage ........................................ Estimated Number of Cells in this Stage …….. Biology – Observing Mitosis Mrs. Douma Stage ........................................ Estimated Number of Cells in this Stage …….. Stage ........................................ Estimated Number of Cells in this Stage …….. Stage ........................................ Estimated Number of Cells in this Stage …….. Animal Cell (Whitefish blastula) Stage ........................................ Estimated Number of Cells in this Stage …….. Stage ........................................ Estimated Number of Cells in this Stage …….. Stage ........................................ Estimated Number of Cells in this Stage …….. Biology – Observing Mitosis Mrs. Douma Stage ...................................... Estimated Number of Cells in this Stage …….. Stage ........................................ Estimated Number of Cells in this Stage …….. Analysis 1. How does Mitosis differ in plant cells and animal cells? 2. Which phase of mitosis shows the greatest difference between animal cells and plant cells? Explain your choice. 3. Why is the onion root a good specimen for studying mitosis? 4. The majority of the cells in your specimens were in which stage of the cell cycle? 5. In Allium, Interphase lasts about 15 hours, and mitosis takes up 80 minutes. Assuming that each stage of mitosis takes the same amount of time, how many hours old is a cell that is just starting anaphase. (Yes, you'll need to do some algebra here - show your work) Biology – Observing Mitosis Mrs. Douma 6. Which stage of mitosis is the easiest (in your opinion) to see on the slide? What about it makes it easy to identify?
