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Proteases ⢠Proteases are the most widely used enzymes in the detergent industry. ⢠They remove protein stains such as grass, blood, egg and human sweat. These organic stains have a tendency to adhere strongly to textile fibres. ⢠The proteins act as glues, preventing the waterborne detergent systems from removing some of the other components of the soiling, such as pigments and street dirt. ⢠The inefficiency of nonenzymatic detergents at removing proteins can result in permanent stains due to oxidation and denaturing caused by bleaching and drying. ⢠Blood, for example, will leave a rustcoloured spot unless it is removed before bleaching. ⢠Proteases hydrolyse proteins and break them down into more soluble polypeptides or free amino acids. As a result of the combined effect of surfactants and enzymes, stubborn stains can be removed from fibres.