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Max E. Joffe [email protected] 443-794-7632 Vanderbilt Center for Neuroscience Drug Discovery Vanderbilt University 12475E Medical Research Building 4 Nashville, TN 37203 EDUCATION August 2011 – April 2016 Vanderbilt University Medical Center Ph.D. Pharmacology August 2006 – May 2010 University of Maryland, College Park B.S. with Honors Citation Major: Mathematics, Minor: Physics RESEARCH EXPERIENCE Postdoctoral Research Fellow, Neuropharmacology lab of Dr. P. Jeffrey Conn, May 2016 – present Pharmacology Department / Vanderbilt University Evaluating mGlu3 modulation as a potential treatment for psychiatric disorders Probing the function of mGlu3 in synaptic physiology, plasticity, and behavior through a combination of techniques including input- and cell type-specific whole cell electrophysiology PhD. Candidate, Behavioral Neuroscience lab of Dr. Brad A. Grueter, December 2012 – April 2016 Anesthesiology Department / Vanderbilt University Medical Center Examined circuit-specific NAc glutamatergic function using viral-delivered optogenetics and targeted whole-cell patch clamp electrophysiology Used BAC transgenic D1/A2A genetic deletions to examine the cell type-specific contributions of NMDAR subunits to reward-related behaviors Graduate Student, Neuropharmacology lab of Dr. Carrie K. Jones, January 2012 – November 2012 Pharmacology Department / Vanderbilt University Evaluated the efficacy of mGlu5 negative modulators to attenuate the reinforcing properties of cocaine Developed procedures in MedState Notation to execute custom operant behavior tasks Performed analyses via MATLAB to evaluate spatial organization of locomotor behavior in mAChR KO mice and drug-treated animals Graduate Rotation, Neuropharmacology lab of Dr. Gregg D. Stanwood, November 2011 – December 2011 Pharmacology Department / Vanderbilt University Assessed the behavioral effects of SKF-83959, a Gq-preferring dopaminergic agonist, in genetic mouse models in locomotor activity assays and the tail suspension test Used immunohistochemistry to examine changes in downstream protein phosphorylation following administration of SKF-83959 in mice Graduate Rotation, Mass Spectrometry lab of Dr. Kevin L. Schey, September 2011 – October 2011 Biochemistry Department / Vanderbilt University Used MALDI-TOF Imaging MS/MS to quantify the distributions and abundances of several ophthalmologic agents and their metabolites in whole mouse eye Performed pilot experiments to detect energy metabolites (e.g. nucleotide phosphates, glutathione) in two-dimensional space within bovine lens tissue using LTQ Imaging MS/MS Data Analyst, Cognitive Science lab of Dr. Deborah A. Zarin, November 2010 – August 2011 ClinicalTrials.gov / National Library of Medicine, contracted by ICF International Extracted data from published articles; organized and summarized findings Provided technical support in the preparation of data quality reports and scholarly communications Collected, analyzed, and summarized data from ClinicalTrials.gov records Special Volunteer, Behavioral Pharmacology lab of Dr. Jonathan L. Katz, September 2010 – May 2011 Medications Discovery Research Branch / National Institute on Drug Abuse Quantified behavioral effects of cocaine withdrawal and established pilot protocol for conditioned place preference in Planaria Assisted with running rat behavioral assays and subsequent data analyses: 5-CSRTT, Delay Discounting, Drug Discrimination and Progressive Ratio Reinforcement Assistant, Metabolic Engineering lab of Dr. Ganesh Sriram, August 2009 – May 2010 ENCH 468, University of Maryland Measured “isotopomer” abundances via data analysis of 2D (1H-13C) NMR experiments Wrote MATLAB programs to quantify and deconvolute NMR multiplets via simulated annealing Intern, Biophysics lab of Dr. Patrick H. Brown, June 2009 – August 2009 Dynamics of Macromolecular Assembly / National Institute on Biomedical Imaging and Bioengineering Characterized nanoparticles (organic and aqueous Quantum Dots & 10-, 20-, 30-nm colloidal Au) Learned and utilized advanced biophysical techniques (Analytical Ultracentrifugation, Dynamic Light Scattering, Field Flow Fractionation, Isothermal Titration Calorimetry, Refractive Index Increment, Dry Weight Measurement, UV-visual Mass Spectrometry) Assistant, Neuroscience lab of Dr. Catherine E. Carr, August 2008 – May 2009 BSCI 399, University of Maryland Sliced, fixed, and stained brain sections; used Neurolucida to construct 3D models of brains Began work on a comparative study of Bowerbird brain size, structure, and organization TEACHING EXPERIENCE Seminar in College Teaching, August 2016 – October 2016 Guest Lecture, September 29, 2016 Vanderbilt University NURO 8352 Methods and Experimental Design in Neuroscience Research “Murine neurobehavior: reward and reinforcement” Guest Lecture, March 1, 2016 Vanderbilt University NSC 3235 Biological Basis of Mental Disorders “Substance abuse and the reward circuitry” Academic Dean’s Assistant, June 2010 – August 2010 Teaching/Program Assistant, June 2007 – August 2007 / June 2008 – August 2008 Johns Hopkins University / Center for Talented Youth Worked with the academic dean on day-to-day administration of the academic program Tutored and supervised elementary, middle, and high school students Assisted with teaching lessons, administrative duties, and final student evaluations Section Leader / Homework Grader / Tutor, August 2008 – December 2009 University of Maryland, College Park / Honors, Mathematics, and Athletic Departments Taught HONR100, a discussion-based course, to seventeen first-semester Honors students Organized a service learning project; designed a research project; led several field trips Graded for Calculus I, III, and Number Theory, between 20-40 students/semester MENTORING EXPERIENCE 2014-2015 Sophie Vitter, undergraduate student, mouse behavioral assays 2016 Douglas Shaw, research assistant, stereotaxic injections HONORS AND AWARDS 2010 University of Maryland Honors Citation 2014 Middle Tennessee Chapter for the Society of Neuroscience Data Blitz Best Presentation 2014 Vanderbilt Graduate Student Travel Award 2015 Vanderbilt Graduate Student Travel Award 2016 Vanderbilt Kennedy Center Science Day Best Abstract, Cellular and Molecular Neuroscience 2016 CNS Drug Discovery Research Training Grant, 5T32MH093366-05 APPOINTMENTS AND COMMITTEES 2014-2016 Review Committee for Vanderbilt Pharmacology Journal Club 2015 Vice President for Community Affairs, Vanderbilt Pharmacology Graduate Student Association 2015 Vanderbilt Pharmacology Student-Invited Form Committee 2016 Chair of Vanderbilt Pharmacology Student-Invited Forum Committee PROFESSIONAL MEMBERSHIPS 2012-2016 Vanderbilt Pharmacology Graduate Student Association 2012Society for Neuroscience 2014Middle Tennessee Chapter for the Society for Neuroscience 2015Tennessee Physiological Society PEER-REVIEWED PUBLICATIONS Joffe ME and Grueter BA. Cocaine experience enhances thalamo-accumbens N-methyl-D-aspartate receptor function. Biological Psychiatry. doi:10.1016/j.biopsych.2016.04.002 Joffe ME, Vitter SR, Grueter BA. GluN1 deletions in D1- and A2A-expressing cell types reveal distinct modes of behavioral regulation. Neuropharmacology. doi:10.1016/j.neuropharm.2016.03.026 Gould RW#, Amato RJ#, Bubser M, Joffe ME, Nedelcovych M, Gray AT, Nickols H, Yuh J, Zhan X, Felts AS, Rodriguez AL, Morrison RD, Byers FW, Rook JM, Daniels JS, Niswender CM, Conn PJ, Emmitte KA, Lindsley CW, Jones CK. Partial mGlu5 negative allosteric modulators attenuate cocaine-mediated behaviors and lack psychotomimetic-like effects. Neuropsychopharmacology. 2016 Mar;41(4):1166-78. doi:10.1038/npp.2015.279 REVIEWS Joffe ME, Grueter CA, Grueter BA. Biological substrates of addiction. WIREs Cogn Sci. 2014, 5:151–171. doi: 10.1002/wcs.1273 Nargund S#, Joffe ME#, Tran D, Tugarinov V, Sriram G. Nuclear magnetic resonance methods for metabolic fluxomics. In Alper HS, Ed., Systems Metabolic Engineering: Methods and Protocols, Methods in Molecular Biology, vol. 985: 335-51. Humana Press. 2013 (#: equal contribution) ORAL PRESENTATIONS “Mechanisms of mGlu3-mediated synaptic plasticity in the prefrontal cortex.” Vanderbilt Kennedy Center Science Day. 9/19/2016. “Cocaine enhances NMDAR function at mThal-NAc core synapses.” Vanderbilt Anesthesiology Research Division Works in Progress. 1/28/2016. “Cocaine exposure generates pathophysiological adaptations at mThal-NAc synapses.” Vanderbilt Pharmacology Works in Progress. 4/21/2015. “Circuit-specific NAc iGluR function and implications for cocaine-related learning.” Vanderbilt Neuroscience Program in Substance Abuse. 12/1/2014. “Relapse-related pathophysiology of the thalamo-accumbens circuit.” Vanderbilt Pharmacology Annual Retreat. 10/28/2014. “NMDA receptor modulation of NAc synaptic and behavioral plasticity.” Vanderbilt Anesthesiology Research Division Works in Progress. 5/15/2014. “Deconstructing nucleus accumbens circuit functions with cell type-specific NMDA receptor deletions.” Middle Tennessee Chapter of the Society for Neuroscience Annual Meeting. 5/14/2014. “NMDA receptor modulation of NAc synaptic and behavioral plasticity.” Vanderbilt Basal Ganglia Club. 2/13/2014. ABSTRACTS Joffe ME, Engers JL, Lindsley CW, and Conn PJ. Mechanisms of mGlu3-mediated synaptic plasticity in the prefrontal cortex. Vanderbilt Kennedy Center Science Day. Nashville, TN. 9/19/2016. Joffe ME and Grueter BA. Cocaine exposure alters thalamo-accumbens synapses. Gordon Research Conference. Ventura, CA. 2/27/2016. Grannan MD, Folkes O, Gould RW, Joffe ME, Bubser M, Bridges TM, Wess J, Ramsey AJ, Wood MR, Grueter BA, Conn PJ, Lindsley CW, Jones CK. Effects of the M4 positive allosteric modulator VU0467154 on cognition and electrophysiological properties in the mPFC in a rodent model of NMDA receptor hypofunction. Vanderbilt Pharmacology Annual Retreat. Nashville, TN. 10/29/2015. Joffe ME and Grueter BA. Cocaine exposure alters thalamo-accumbens synapses. Society for Neuroscience Annual Meeting, Chicago, IL 10/19/2015. Joffe ME, Vitter SR, and Grueter BA. NMDAR function in striatal neurocircuits regulates despair and cocaine reinstatement. Vanderbilt Department of Anesthesiology Research Retreat. 5/1/2015. Joffe ME and Grueter BA. Deconstructing nucleus accumbens circuit functions with cell type-specific NMDA receptor deletions. Society for Neuroscience Annual Meeting, Washington, D.C. 11/19/2014. Joffe ME and Grueter BA. NMDA receptor modulation of NAc synaptic and behavioral plasticity. Vanderbilt Department of Anesthesiology Research Retreat. 5/3/2014. Amato RJ, Joffe ME, Morrison RD, Rodriguez AL, Felts AS, Emmitte KA, Daniels JS, Conn PJ, Lindsley CW, Jones CK. Potential of partial negative allosteric modulators of the metabotropic glutamate receptor subtype 5 for reversal of cocaine self-administration in rats. Society for Neuroscience Annual Meeting, New Orleans, LA. 10/17/2012. Nargund S, Joffe ME, Tugarinov V, Sriram G. Metabolic Flux Analysis Using Designed Isotope Labeling Experiments Reveals Effects of Light On Metabolic Fluxes in Heterotrophic Plant Suspension Cells. American Institute of Chemical Engineers. 10/16/2011.