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ORGANISMS: A unit of living matter capable of independent function and reproduction Complex organisms-an organism with cell tissues, and organs arranged to function as a single unit -Example: plants, humans, animals. ORGAN: -An arrangement of tissues which provides life support functions for an organism -Ex: a leaf in a plant or a heart in an animal TISSUE: -Arrangement of cells with similar structures in clusters or sheets for specific functions in an organ CELL: -Smallest structural unit of living matter than is capable of carrying out basic life processes. -Discovered by Robert Hooke in 1665 -Basic building block of life -Most basic life processed takes place at the cellular level. HOW ERE CELLS UNDERSTOOD? -Electron microscope- uses beams of electrons rather than beams of light to illuminate the object. -Allowed scientists to see how cells function PROKARYOTIC CELLS: -Single celled -Contains genetic material -Not confined to the nucleus. -Neither plant nor animal -Smallest of all the cells -Bacterium -Value to biotechnology -Used to make cheese and ferment wine -Used in medicine -Genetically engineered bacteria that carry genetic material for insulin production -BST id produced by genetically altered bacteria -Growth hormone used to make cows produce more milk -Bovine somatopin -Since bacteria multiply rapidly, large quantities of these substances can be produced. EUKARYOTIC CELLS: What plants and animals are made of -Have a nucleus and membrane-bound organelles THE NUCLEUS: -Control center for all activities of the cell -Most cells have one nucleus -Muscle cells may have may nuclei CELL MEMBRANE: -Encloses and protects the cells contents from the eternal environment -Regulates the movement of materials into and out of the cell -Taking in nutrients -Expelling wastes -Allows interaction with other cells -Animals: plasma membrane -Plants: cell wall CYTOPLASM: -Fluid that surrounds the nucleus -Contains all the materials needed by the cell to conduct life processes -Contains the organelles DNA: -Deoxyribonucleic Acid -Contains the genetic code -Double helix founded by Watson and Crick in 1953 Base pairs to form DNA “ladder” -Nucleotides paired through hydrogen bonds -Adenine – Thymine -Guanine – Cytosine CHROMOSOMES: -Structures that are physical carriers of genes, are made up of DNA, and are located in the cell nucleus MITOCHANDRIA: -Energy-producing structures in the cell cytoplasm that contain some DNA. (ATP) -Powerhouse of the cell PLASMID: -Extra chromosomal piece of DNA located in the cell cytoplasm RIBOSOME: -Site where new proteins are produced as a result of genetic expression -Assemble the amino acid molecules IMPORTANCE OF CELLS: -Agr. Production begins with cells -Healthy and unhealthy growth of plants and animals -Tissues are composed of a group of specialized cells that allow the tissue to serve it function -Skeletal tissue, connective tissue, etc. ELECTRON MICROSCOPE: -1940’s and 1950’s- understanding of the anatomy and functions of cells was greatly increased -Uses beans of electrons to illuminate a cell -We use compound microscopes -Allowed u to look inside of a cell and see all of its components CELL REPRODUCTION: -Continuation of life is dependent on cell reproduction -Used for reproduction and growth -The difference between it is that meiosis is for sex cells and all the others are mitosis -Mitosis is used for growth, meiosis is used for reproduction -Both are used for gene splicing, genetic engineering, and other biotech MITOSIS: -All growth in living organisms depends on mitosis -Cells are going to increase in either size of numbers -Used to heal wounds in either plants or animals -Cells will grow until they reach their maximum size -When this is reached the cells will divide into two cells and this process continues -Parent cell INTERPHASE: -Period when the cell is not actively dividing -Cell is carrying on processes such as synthesizing materials and moving them in and out of the cell -Cell grows to it’s maximum size -DNA is replaced and forms two complete sets of chromosomes -Thread like molecules of DNA make up the chromosomes are called chromatin and are spread throughout the nucleus. -Cell is read to divide PROPHASE: -First stage -Chromatin appears in the form of distinct, shortened rod-like structures -Chromosomes are formed of the two strands called chromatids, attached at the center called a Centromere -Nuclear membrane is dissolving and entire nucleus is disappearing -Spindle is formed in place of the nucleus -Football like shaped structure -Centrioles move to the opposite ends of the cell METAPHASE: -Chromatid move toward the center of the spindle (the equator) -When they reach the center, the centromeres connect themselves to the spindle fibers ANAPHASE: -Pairs of chromatids separate into an equal number of chromosomes and the centromeres duplicate -After separation, chromosomes move to the sides of the cell TELOPHASE: -Final phase -Chromosome migrate to poles of the cell -Spindle disappears -Membrane formed around chromosomes (nuclear membrane) -Two nuclei formed CYTOKINESIS: -2 fresh new daughter cells identical to the parent cell -Cells are back in interphase -Organisms can grow and repair MEIOSIS: -Cells are divided into cells that contain only ½ of chromosome needed for the formation of a young animal -SEX CELLS -Testicles-form sperm -Ovaries-form the egg STEP ONE: REPLICATION -Chromosomes make an exact copy of themselves -Replicated chromosomes are called chromatids STEP TWO: SYNAPSIS -Chromatids come together and are matched up in pairs STEP THREE: -Cell divides and the chromosomes are separated -Each cell receives one of each chromosome from each pair STEP FOUR: -Both the cells and the chromatids separate and the chromatids become chromosomes -End result: four sperm cells from one original reproductive cell -Each one contains half the number of chromosome that the original sex cell had SPERMATOGENESIS; -Production of the male gamete OOGENESIS: -Production of the female gamete -Same as the process of spermatogenesis except that only one sex cell is created -Polar bodies are created because production of one egg cell takes so much nourishment/energy ONCE FERTILIZATION HAS OCCURRED: -A complete cell has formed with all the genetic material necessary for its development into a complete organism -The cells are totipotent -Totipotency-capability of a cell to develop into any type of cell -The cells then divide and group together into what is called a morula -Cleavage- process where the cells in the morula keep dividing and clump together -Blastula: morula gets larger and forms a spherical shape with an outer layer and an inner mass of cells -Outer layer-placenta -Inner masses: different types of body tissues -Once the blastula develops and grows, cells begin to take on different characteristics which then leads to organs-cell differentiation -Stem cells-cells that begin the differentiation process STEM CELLS: -Hopefully in the future will be used to create new tissue to replace disease or damaged human tissue -Diseases such as Parkinson’s, diabetes, and heart disease could be cured.