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Portland Community College, Sylvania Campus BI 231 Lab Supplemental Package 1 PCC-Sylvania BI 231 Laboratory Supplement 1. Upon entering the laboratory, please locate the exits, fire extinguisher, eyewash station, and clean up materials for chemical spills. Your instructor will demonstrate the location of fire blanket, safety kit, and showers. 2. Read the general laboratory directions and any objectives before coming to lab. 3. Food and drink, including water, are prohibited in laboratory. This is per Federal laboratory guidelines and per College Safety Policy. Do not chew gum, use tobacco products of any kind, store food or apply cosmetics in the laboratory. No drink containers of any kind may be on the benches. 4. Please keep all personal materials off the working area. Store backpacks and purses at the rear of the laboratory, not beside or under benches. Some laboratory spaces have shelving in rear for this purpose. 5. For your safety, please restrain long hair, loose fitting clothing and dangling jewelry. Hair ties are available, ask your instructor. Hats and bare midriffs are not acceptable in the laboratory. Shoes, not sandals, must be worn at all times in laboratory. You may wear a laboratory apron or lab coat if you desire, but it is not required. 6. We do not wish to invade your privacy, but for your safety if you are pregnant, taking immunosuppressive drugs or who have any other medical conditions (e.g. diabetes, immunological defect) that might necessitate special precautions in the laboratory must inform the instructor immediately. If you know you have an allergy to latex or chemicals, please inform instructor. 7. Decontaminate work surfaces at the beginning of every lab period using Amphyl solution. Decontaminate bench following any practical quiz, when given, and after labs involving the dissection of preserved material. 8. Use safety goggles in all experiments in which solutions or chemicals are heated or when instructed to do so. Never leave heat sources unattended: hot plates or Bunsen burners. 9. Wear disposable gloves when handling blood and other body fluids or when touching items or surfaces soiled with blood or other body fluids such as saliva and urine. (NOTE: cover open cuts or scrapes with a sterile bandage before donning gloves.) Wash your hands immediately after removing gloves. 10. Keep all liquids away from the edge of the lab bench to avoid spills. Immediately notify your instructor of any spills. Keep test tubes in racks provided, except when necessary to transfer to water baths or hot plate. You will be advised of the proper clean-up procedures for any spill. 11. Report all chemical or liquid spills and all accidents, such as cuts or burns, no matter how minor, to the instructor immediately. 12. Use mechanical pipetting devices only. Mouth pipetting is prohibited. Students who do not comply with these safety guidelines will be excluded from the Laboratory 2 Safe Disposal of Contaminated Materials Place disposable materials such as gloves, mouth pieces, swabs, toothpicks and paper towels that have come into contact with blood or other body fluids into a disposable Autoclave bag for decontamination by autoclaving. This bucket is not for general trash. Place glassware contaminated with blood and other body fluids directly into a labeled bucket of 10% bleach solution. ONLY glass or plastic-ware is to be placed in this bucket, not trash. Sharp’s container is for used lancets only. It is bright red. When using disposable lancets do not replace their covers. 1. Properly label glassware and slides, using china markers provided. 2. Wear disposable gloves when handling blood and other body fluids or when touching items or surfaces soiled with blood or other body fluids such as saliva and urine. (NOTE: cover open cuts or scrapes with a sterile bandage before donning gloves.) Wash your hands immediately after removing gloves. 3. Wear disposable gloves when handling or dissecting specimens fixed with formaldehyde or stored in Carosafe/Wardsafe. 4. Wear disposable gloves when handling chemicals denoted as hazardous or carcinogenic by your instructor. Read labels on dropper bottles provided for an experiment, they will indicate the need for gloves or goggles, etc. Upon request, detailed written information is available on every chemical used (MSDS). Ask your instructor. 5. No pen or pencil is to be used at any time on any model or bone. The bones are fragile, hard to replace and used by hundreds of students every year. To protect them and keep them in the best condition, please use pipe cleaners and probes provided instead of a writing instrument. a. Probes may be used on models as well. The bones are very difficult and costly to replace, as are the models and may take a long time to replace. 6. At the end of an experiment: a. Clean glassware and place where designated. Remove china marker labels at this time. b. Return solutions & chemicals to designated area. Do not put solutions or chemicals in cupboards! 7. You cannot work alone or unsupervised in the laboratory. 8. Microscopes should be cleaned before returning to numbered cabinet. Be sure objectives are clean, use lens paper. Place objectives into storage position, and return to the storage cabinet. Be sure cord has been coiled and restrained. Your instructor may require microscope be checked before you put it away. Be sure it is in assigned cupboard. 9. Please replace your prepared slides into the box from which they came (slides and boxes are numbered), so students using them after you will be able to find the same slide. Before placing slides in box, clean it with Kimwipes if it is dirty or covered with oil. If you break a slide, please, inform you instructor so the slide can be replaced. Please be aware that there is hundreds of dollars worth of slides in each box and handle the boxes with care when carrying to and from your workbench. 10. Be sure all paper towels used in cleaning lab benches and washing hands are disposed of in trash container provided. Students who do not comply with these safety guidelines and directions will be excluded from the Laboratory 3 Please Read You are beginning a very intense laboratory course. Before you come to class you will want to review what the study focus is for that day’s lab. This is important because you will be liable (tested) for the information listed in your study guide and manual. There are lists of terms that you are required to know, as well as tables and diagrams. These are testable as well. If there are slides listed in the study guide then you are also liable to identify these structures under the microscope on quizzes or on practicals. There will also be various models that are available in the classroom which will be used in the tests. It is up to the student to identify the structures on these models. Remember, majority of your practicals will be on these models. Please do not think that you will be able to look at the pictures in the book and do well on quizzes and practicals. YOU NEED TO SPEND TIME WITH THE MODELS! Some labs will have exercises that are required. Please make sure that you understand what was learned in these exercises because these are also fair game to be used for questions in the tests. Each lab will start with a 10 point quiz. You are required to be in attendance at the beginning of each lab. You will receive a zero on the quiz if you miss it. There will not be quizzes on the weeks we have a practical or the week after a practical. If you stay in lab only long enough to take the quiz and then leave soon after the lab will be counted as a missed lab. Spelling can account for up to 10% off of your grade so please be careful. Also be aware of singular and plural usage because these mistakes will count as spelling errors. Absences: You cannot miss more than two labs and still pass the course. Also you can only attend another instructor’s class once during the quarter. This must be approved by both instructors. If you attend another instructor’s lab without permission your quiz will be automatically thrown out. There are review sheets at the end of each exercise that we recommend that you do. You will not receive credit for these pages but they will help you study the material and prepare for the tests. Any material found in the lab manual can be used for the extra credit questions. If you have any questions please contact Marilyn Thomas, Lab Coordinator ([email protected]) Thank you! 4 BI 231 Laboratory: Week 1 Introduction to Anatomy and Physiology Go over Laboratory Guidelines and Procedures, sign-up for microscopes and lab cabinet From Lab Manual Ex 1. Body Organizationn and Terminology Ex 2. Care and Use of the Compound Light Microscope: This chapter is review. You should already know how to use and care for a microscope. Please ask your instructor for help if you need help have forgotten how to use the microscope. ________________________________________________________________________________ Study focus: Anatomical terminology, organ systems and organ DO NOT DO ACTIVITY 2.5 on page 22. Lab resources: Dissectable models, muscle models Anatomical body regions, relationships, and planes (Figs. 1.1, 1.2, 1.3)(Table 1.1) Organs and organ systems (Fig. 1.5) Abdominopelvic quadrants and regions (Fig. 1.6)(Table 1.2) Body cavities (Fig. 1.7)(Table 1.3) Serous membranes (Fig. 1.7) Compound light microscopes: care and use thereof Superficial muscle groups, please label image below a. Sternocleidomastoid b. Deltoid c. Pectoralis major d. External abdominal oblique e. Rectus abdominis f. Biceps brachii g. Sartorius h. Rectus femoris i. Tibialis anterior j. Trapezius k. Deltoid 5 l. Triceps brachii m. Latissimus dorsi n. Gluteus maximus o. Semitendinosus p. Biceps femoris q. Gastrocnemius 6 BI 231 Laboratory: Week 2 Quiz 1 (material from previous week) Tissues – Histology Ex 5. Epithelial and Connective Tissues ________________________________________________________________________________ Study focus: Be able to identify various epithelial and connective tissues and know locations in the human body where these tissues can be found. Remember that organs contain more than one tissue type so majority of the slides that you will view will have more than one tissue type so please use the characteristics of the various tissue types to identify them within the organ you are viewing. Lab resources: Microscopes, slide boxes in designated cabinets, instructor scope Epithelial Tissues: slides to use are outlined in parentheses (Know Table 5.1, 5.2) Simple squamous epithelium (artery, vein, capillaries, alveoli of the lung, Bowman’s capsule) Stratified squamous epithelium, keratinized (skin) and non-keratinized (esophagus) 7 Simple cuboidal epithelium (thyroid gland, kidney tubules, some glandular ducts, sweat glands) Stratified cuboidal epithelium (ducts of mammary glands, salivary glands, sweat glands) Simple columnar epithelium, ciliated (fallopian/uterine tube) and non-ciliated (GI tract) 8 Pseudostratified ciliated columnar epithelium (trachea, bronchi) Transitional epithelium (urinary bladder, ureters) Mucous membranes: epithelial tissues that secrete mucus via goblet cells Connective Tissues: slides are labeled with corresponding tissue (know Table 5.3) Embryonic CT: Mesenchyme (no slide in lab, please use laboratory manual) Connective tissue proper: Loose CT and Dense CT o Loose CT: Areolar tissue, adipose tissue, reticular tissue o o 9 o Dense CT: Dense regular CT, dense irregular CT, elastic tissue Solid connective tissues o Cartilage: Hyaline cartilage, elastic cartilage, fibrocartilage o Bone. Structural components of compact bone (Fig. 5.16) 10 Fluid connective tissues o Blood. Formed elements: erythrocytes, leukocytes, platelets o Lymph (no slide) Matrix of CT = Ground substance + fibers (collagen, elastic, reticular) I recommend drawing the slides that you view under the microscope. This helps clarify distinguishing characteristics of each tissue type. If you do not wish to draw the slides write the functions of the tissues. Terms to know: Collagen fibers* Blast cells Elastic fibers* Fibroblasts Reticular fibers* Chondroblasts Matrix Osteoblasts Ground substance Hemocytoblasts Goblet cells* Fibrocytes Erythrocytes* Chondrocytes* Leukocytes* Osteocytes* *Be able to identify these cells and fibers under the microscope 11 BI 231 Laboratory: Week 3 Quiz 2 (material from previous week) The Integument and its Accessory Structures Ex 6. The Integumentary System: Cutaneous membrane (skin) + accessory structures (pp6979) Ex 7. Part I: Introduction to the Skeletal System (pp. 83 - 88 _______________________________________________________________________________ Study focus: Be able to identify organizational layers of the epidermis and dermis as well as identify accessory structures of the skin Slides available: thick and thin skin slides and compact bone slides Lab resources: Integument models, integument and finger nail models, microscope, slide boxes Microscopic structure of the skin Anatomical model of the skin, images Specialized cells of the skin Accessory structures of the skin The structure of nails Terms to know: Organ level Organ system Organ Cutaneous membrane Accessory structures of skin Integumentary system Epidermis Dermis Thick skin Thin skin Stratum germinativum/ basale Stratum spinosum Stratum granulosum Stratum lucidum Stratum corneum Keratinocytes Papillary layer Reticular layer Dermal papillae Epidermal ridges Hypodermis (superficial fascia or subcutaneous tissue) Stratified squamous, keratinized epithelium Melanocytes Hair follicles Hair bulb Hair matrix Hair root 12 Hair shaft Keratin Sebaceous glands Arrector pili muscle Merocrine (eccrine) sweat glands Apocrine sweat glands Tactile (Meissner’s) corpuscles Lamellated (Pacinian) corpuscles Nail plate Nail bed Free edge Hyponychium Lunula Cuticle or eponychium Lateral nail folds Nail matrix Nail root Exercise 7: Terms to know Fig 7.1 Classification of bones by shape Fig 7.3 structure of compact and spongy bone (All) Lamellae Compact bone Osteon (Haversian system) Spongy (cancellous) bone Trabeculae Red bone marrow Yellow bone marrow Central (Haversian) canal Perforating (Volkmann’s) canals Interstitial lamellae Circumferential lamellae Osteocytes Lacunae Canaliculi Medullary cavity Diploe Axial skeleton Appendicular skeleton Endosteum Periosteum Perforating or Sharpey’s fibers 13 BI 231 Laboratory: Week 4 Quiz 3 (material from previous week) Muscle Tissue – Histology & Nervous Tissue – Anatomy and Histology Ex 10. Histology of Muscle Tissue Ex 13. Histology of Nervous Tissue (pages 249-255) Review for Lab Practical I ________________________________________________________________________________ Study focus: Muscle and nervous tissue histology taken from exercises 10 and 13 Lab resources: Model of neuron, model of muscle w/neuromuscular junction, microscope, slide boxes Slides available: Neuron, nerve, skeletal, cardiac and smooth muscle slides Terms to know from exercise 10: Skeletal muscle Cardiac muscle Smooth muscle Skeletal muscle fibers epimysium Endomysium Perimysium Fascicle Striations Myofibrils I bands A band H band Intercalated discs Actin (thin filaments) Myosin (thick filaments) Sarcoplasm Draw and label a sarcomere: Action potentials M line Zones of overlap Z lines Sarcomere Sarcolemma Neuromuscular junction Neurotransmitter Acetylcholine (Ach) Acetylcholinesterase (AChE) Motor nerve fiber (axon) Synaptic terminal Motor end plate Motor unit 14 Muscle tissue (skeletal, smooth, cardiac) Terms to know from Exercise 13 Table 13.1 Neuroglia cell types Central nervous system (CNS) Peripheral nervous system (PNS) Cranial nerves Spinal nerves Receptors Motor (efferent) division Sensory (afferent) division Somatic nervous system Autonomic nervous system Somatic sensory receptors Visceral sensory receptors Special sensory receptors Neuron or nerve cell Chromatophilic (Nissl) bodies Nuclei (singular = nucleus) Ganglia (singular = ganglion) Dendrites Axons Synaptic (axon) terminal (synaptic knob) Myelin sheath Neurolemmocytes (Schwann cells) Oligodendrocytes Myelinated fibers Unmyelinated fibers Nodes (nodes of Ranvier) Interneurons or association neurons Multipolar neurons Anaxonic neurons Bipolar neurons Unipolar neurons Pseudounipolar neurons Neuroglia or glial cells Cell body Axon hillock Synapses axoplasm 15 Lab Practical I will be next week (week 5)! The practical will cover all the material discussed in the last 4 weeks of lab Terms to know, labeled figures, tables, any additional info outlined in the package Models Images Microscopes (images will also be provided for histology questions) 75 questions Timed stations 16 BI 231 Laboratory: Week 6 No quiz this week! The Axial Skeleton Ex 7. Part 2, The Axial Skeleton (pp. 89-110) _____________________________________________________________________________________ Study focus: Be able to identify all bones and landmarks on tables 7.1, 7.2, 7.3 and 7.4 Lab resources: Skulls, bags of ribs, sternum, box of vertebrae, boxes of articulated spines. Also, there is a fetal skull and a model of disarticulated skull (please leave on the instructor bench) For clarification: Zygomatic arch: Zygomatic process (temporal bone) + Temporal process (zygomatic bone) Nasal septum: Perpendicular plate (ethmoid bone) + vomer Terms to know: Along with the Tables listed above Frontal sinus Sphenoidal sinus Maxillary sinus Ethmoid sinus Anterior (frontal) fontanel Posterior (occipital) fontanel Anterolateral (sphenoidal) fontanel Posterolateral (mastoid) fontanel Primary curvatures Secondary curvatures Intervertebral foramen Atlas Axis Dens Sternum Manubrium Body Xiphoid process Sternal angle Costal cartilages True ribs False ribs Vertebrochondral ribs Floating ribs Vertebral end of rib Sternal end of rib Head, tubercle, neck, shaft or body, angle and costal groove of ribs Coronal suture Squamous suture Sagittal suture Lambdoid suture Nuchal lines of occipital bone Alveolar ridge Lateral, medial and superior borders of scapula 17 Study Tips and How to cope with the amount of material for the following labs Write out a list of terms Write out terms as many times as it takes you to get the spelling correct Test yourself …you can do this in many ways! Former successful students have o Made up quizzes near the end of lab using models o Had other students quiz them using models or – if not present – images o Taken pictures of models/specimen and made up a PowerPoint quiz o Drawn structures and named them If you do the above, test yourself randomly by using several models at once …remember that in the practical you will have to recall names of structures in a random fashion! If you do the above, write down the structures you are naming to practice spelling! If a name is hard for you to remember, it helps to know the meaning of root words and to make the connection to the structure… after all, they have a name for a reason Some student use mnemonics to remember names for structures Taking pictures of models always helps! There are also great web resources with labeled images of the models we use in lab Use a coloring book! Successful students have raved about them… 18 BI 231 Laboratory: Week 7 Quiz on the Axial Skeleton Ex 8. Part 2, The Appendicular Skeleton Ex 9: The structure of the knee joint _____________________________________________________________________________________ Study focus: Know all the bones and landmarks listed in Tables 8.1 and 8.2, fig. 8.9 Lab resources: Articulated skeletons, bone boxes Terms to know: Male and female pelvis characteristics (See fig 8.8) Hyoid bone Interosseous membrane Quadriceps tendon Patellar ligament Tibial collateral ligament Fibular collateral ligament Anterior cruciate ligament Posterior cruciate ligament Medial meniscus Lateral meniscus 19 BI 231 Laboratory: Week 8 Quiz on the Appendicular Skeleton Ex 11. Part 1, The Muscle System _____________________________________________________________________________________ Study focus: List of muscles included in this packet ONLY found in Exercise 11 on pp. 166 - 187 of lab manual, Exercise 11 review sheet Lab resources: Models, Muscle men Give definition of origin of a muscle: Give definition of insertion of a muscle: Table 9.2 Anatomical terms of movement FlexionExtension Abduction Adduction SupinationPronationOppositionRepositionEversionInversion Protraction Retraction Elevation Depression Dorsiflexion Plantar flexion Medial rotation Lateral rotation Circumduction Lateral flexion 20 Be able to identify the muscle, action, origin and insertion for each muscle Muscles MUSCLES OF THE HEAD ORIGIN INSERTION ACTION NERVE Frontalis Epicranial aponeurosis Skin over forehead Elevates eyebrows and wrinkles skin of forehead Facial VII Occipitalis Nuchal line on occipital bone Epicranial aponeurosis Fixes epicranial aponeurosis and pulls scalp posteriorly Facial VII Orbicularis oculi Medial orbital margin Skin surrounding eye Closes eyelids and acts during squinting and blinking Facial VII Temporalis Temporal bone Coronoid process of mandible Elevates and retracts mandible Trigeminal V Masseter Zygomatic process and arch Ramus of mandible Elevates mandible Trigeminal V Orbicularis oris Maxillary bone; mandible lips Closes and purses lips Facial VII Zygomaticus major Zygomatic bone Corner of mouth Elevates corner of mouth Facial VII Mentalis Alveolar ridge of incisors of mandible Skin over chin Elevates and protrudes lower lip Facial VII Risorius Fascia of masseter Corner of mouth Draws corner of mouth laterally Facial VII 21 SUPERFICIAL MUSCULATURE OF THE NECK ORIGIN INSERTION ACTION NERVE Omohyoid Superior belly Clavicle and first rib Hyoid bone Depresses hyoid bone Ansa cervicalis Inferior belly Superior margin of scapula Clavicle and 1st rib Thyrohyoid Thyroid cartilage of the larynx Hyoid bone Depresses hyoid bone, elevates thyroid cartilage Ansa cervicalis Sternothyroid Posterior surface of manubrium Thyroid cartilage of the larynx Depresses thyroid cartilage Ansa cervicalis Sternohyoid Posterior surface of manubrium, and medial clavicle Hyoid bone Depresses hyoid bone Ansa cervicalis Stylohyoid Styloid process Hyoid bone Moves hyoid bone up and back Facial VII Mylohyoid Inferior margin of mandible Hyoid bone Elevates hyoid bone and floor of mouth, depresses mandible Trigeminal V Digastric Anterior: Lower border of mandible near midline Hyoid bone Elevates hyoid bone and base of tongue, depresses mandible Ant: Trigeminal V - Anterior belly -Posterior belly Posterior: Mastoid process of temporal bone 22 Post: Facial VII MUSCLES OF THE NECK & BACK ORIGIN INSERTION ACTION NERVE Sternocleidomastoid Manubrium and medial third of clavicle Mastoid process Flexion and lateral flexion of neck; rotates head so face is turned superiorly and to the opposite side C2 and C2, spinal accessory, XI Trapezius Posterior surface of occipital bone; ligamentum nuchae; and spinous process of thoracic vertebrae Lateral third of clavicle; acromion process; spine of scapula Superior fibers: elevates scapula Spinal accessory XI, C3, and C4 Middle fibers: retracts scapula Inferior fibers: depress scapula Latissimus dorsi Spinous processes of lower 6 thoracic vertebrae, thoracolumbar fascia, crest of ilium Intertubercular groove of humerus Extension, adduction and medial rotation of arm Thoracodorsal Levator scapula Transverse processes of C1-4 Medial border above spine of scapula Elevates scapula; acts with pectoralis minor to inferiorly rotate scapula Doral scapular Rhomboid major Spinous process of T2-5 and supraspinous ligament Medial border below spine of scapula Adducts scapula and rotates it downward; stabilizes scapula Dorsal scapular Rhomboid minor Spinous process of C7-T1 Medial border of scapula at base of spine Adducts scapula and rotates it downward; stabilizes scapula Dorsal scapular Serratus anterior External surface of upper 8 ribs Medial border of scapula Abducts scapula and rotates it upward. Important in horizontal arm movements (‘boxer’s muscle) Long thoracic 23 Sacrum, iliac crest, spinous processes of lumbar vertebrae and T11,12 Angle of the last 6 or 7 ribs Extension of vertebral column Spinal nerves dorsal rami --Longissimus Sacrum, iliac crest, spinous processes of lumbar vertebrae and T11,12 Transverse processes of thoracic vertebrae Extension of vertebral column Spinal nerves dorsal rami --Spinalis Sacrum, iliac crest, spinous processes of lumbar vertebrae and T11,12 Spinous processes of upper thoracic vertebrae Extension of vertebral column Spinal nerves dorsal rami Ant. and middle: elevates 1st rib, flexes neck forward and laterally, rotates neck Cervical spinal C3-C8. Erector Spinae --Iliocoastalis Scalenes - Anterior Middle Posterior MUSCLES OF THORACIC WALL Transverse processes of C3-C6 1st rib Transverse processes of C2-C7 1st rib Transverse processes of C4-C6 2nd rib ORIGIN Post: elevates 2nd rib, flexes neck laterally, slightly rotates neck INSERTION ACTION NERVE External intercostal Inferior border of rib above Superior border of rib below Elevates rib cage during normal inspiration Intercostal Internal intercostal Superior border of rib below Inferior border of rib above Depresses rib cage during forced expiration Intercostal 24 Pectoralis major Medial half of clavicle, sternum, costal cartilages, aponeurosis of external abdominal oblique Lateral lip of intertubular groove of humerus Flexion, medial rotation and adduction of the arm Lateral and medial pectoral Pectoralis minor Anterior surface of ribs 3 to 5 Coracoid process of scapula Depression and inferior rotation of scapula Medial pectoral MUSCLES OF THE ANTERIOR ABDOMINAL WALL ORIGIN INSERTION ACTION NERVE Rectus abdominis Pubis symphysis and crest of pubis Xiphoid process and cartilages of ribs 5 to 7 Flexion of vertebral column; stabilizes pelvis during walking; compresses abdomen to aid in defecation, urination, forced exhalation. Intercostal 7 to 12 External abdominal oblique External surface of lower 8 ribs Linea alba; pubic crest; pubic tubercle; iliac crest Rotation and lateral flexion of trunk; increases intraabdominal pressure during forced expiration Intercostals 8 to 12, iliohypogastric, ilioinguinal Internal abdominal oblique Lateral half of inguinal ligament, anterior iliac crest and thoracolumbar fascia Lower four ribs, linea alba and by conjoined tendon to pubis Compresses abdomen, and flex vertebral column; acting singly, laterally flex vertebral column Intercostals 8 to 12, iliohypogastric, ilioinguinal Transverse abdominis Lateral third of inguinal ligament, anterior iliac crest, and thoracolumbar fascia Linea alba, pubis crest Compresses abdomen and Increases intraabdominal pressure during forced expiration Intercostals 7 to 12, iliohypogastric, ilioinguinal 25 MUSCLES OF THE SHOULDER AND ARM Deltoid ORIGIN lateral third of clavicle, acromion process and spine of scapula INSERTION Deltoid tuberosity of humerus ACTION Anterior fibers: medial rotation and flexion of arm NERVE Axillary Middle fibers: abduction of arm Posterior fibers: lateral rotation and extension of arm Supraspinatus Supraspinous fossa Superior portion of greater tubercle of humerus Abducts humerus Suprascapular Infraspinatus Infraspinous fossa Middle portion of greater tubercle of humerus Rotates humerus laterally Suprascapular Teres minor Lateral border of scapula Greater tubercle of humerus Rotates humerus laterally Scapular circumflex Subscapularis Subscapular fossa Lesser tubercle of humerus Rotates humerus medially Upper and lower subscapularis Teres major Scapula, near inferior angle Medial lip of intertubular groove of humerus Extends arm at shoulder joint and assists in adduction and medial rotation of arm at shoulder joint. Lower subscapular Biceps brachii Long head, supraglenoid tubercle; Tuberosity of radius Flexes and supination of forearm; flexion of arm Musculocutaneous Coronoid process of ulna Flexes forearm Musculocutaneous and radial Short head, coracoid process scapula Brachialis Anterior distal two-thirds of humerus 26 Coracobrachialis Coracoid process of scapula Middle third of humerus Flexes and adducts arm at shoulder joint Musculocutaneous nerve Triceps brachii Long head, infraglenoid tubercle; Olecranon process of ulna Extension of forearm; long head extends and adducts arm Radial Lateral head, proximal portion of posterior humerus; Medial head, distal half of posterior humerus 27 BI 231 Laboratory: Week 9 Quiz on Muscle Group 1 Ex 11. Part 2, The Muscle System _____________________________________________________________________________________ Study focus: List of muscles included in this packet ONLY found in exercise 11 on pp. 187 - 232 of lab manual, Exercise 11 review sheet Lab resources: Models, Muscle men Be able to identify the muscle, action, origin and insertion for each muscle Anconeus Lateral epicondyle of humerus Olecranon process, posterior surface of ulna 28 Weak extensor of ulna, stabilizes elbow joint in extension Radial MUSCLES OF ANT. FOREARM ORIGIN INSERTION ACTION NERVE Flexor carpi radialis Medial epicondyle of humerus Base of second metacarpal Flexion and abduction of the wrist Median Flexor carpi ulnaris Medial epicondyle of humerus, olecranon process, & posterior ulna Pisiform, hamate, and fifth metacarpal Flexes and adducts wrist Ulnar Pronator teres Medial epicondyle of humerus and coronoid process of ulna Lateral surface of radius Pronates and flexes forearm Median Flexor digitorum superficialis Medial epicondyle of humerus and coronoid process of ulna Middle phalanges of fingers Flexes fingers and wrist Median Flexor digitorum profundus Anterior and medial surfaces of ulna and interosseous membrane Distal phalanges of fingers Flexes fingers and wrist Median and Ulnar Flexor pollicis longus Middle half of radius, interosseous membrane, coronoid process of ulna Distal phalanx of thumb Flexes thumb and wrist Median Palmaris longus Medial epicondyle of the humerus Palmar aponeurosis Weak flexion of wrist Median 29 MUSCLES OF POSTEROLATERAL FOREARM ORIGIN INSERTION ACTION NERVE Brachioradialis Lateral supracondylar ridge Styloid process of radius Flexes forearm Radial Extensor carpi radialis longus Lateral supracondylar ridge of humerus Second metacarpal Extends and abducts wrist Radial Extensor carpi radialis brevis Lateral epicondyle of humerus Third metacarpal Extends and abducts wrist Radial Extensor digitorum Lateral epicondyle of humerus Distal and middle phalanges of each finger Extends fingers and hand Radial Extensor digiti minimi Lateral epicondyle of humerus Tendon of extensor digitorum on fifth phalanx Extends 5th digit and hand Radial Extensor carpi ulnaris Lateral epicondyle of humerus and posterior border of ulna Fifth metacarpal Extends and adducts hand at wrist joint Radial Abductor pollicis longus Posterior surface of ulna and radius, and interosseous membrane First metacarpal Abducts and extends thumb Radial Extensor pollicis brevis Posterior surface of middle of radius and interosseous membrane Base of proximal phalanx of thumb Extends thumb Radial Extensor pollicis longus Posterior surface of middle third of ulna and interosseous membrane Base of distal phalanx of thumb Extends thumb Radial Supinator Lateral epicondyle of humerus, proximal ulna Lateral surface and posterior border of radius Supinates forearm Radial 30 MUSCLES OF THE PALM ORIGIN INSERTION ACTION NERVE Abductor pollicis brevis Scaphoid and trapezium Proximal phalanx of thumb Abduction of thumb; opposition of thumb Median Flexor pollicis brevis Trapezium Proximal phalanx of thumb Flexes thumb; opposition of thumb Median Flexor digiti minimi Hook of hamate Proximal phalanx of fifth digit Flexes fifth digit Ulnar Abductor digiti minimi Pisiform and tendon of flexor carpi ulnaris Proximal phalanx of fifth digit Abducts fifth digit Ulnar MUSCLES OF DEEP BACK AND GLUTEAL REGION ORIGIN INSERTION ACTION NERVE Gluteus maximus Upper portion of ilium, the sacrum and coccyx Gluteal tuberosity and iliotibial tract Principal extensor and lateral rotator of thigh; Inferior gluteal Gluteus medius Lateral surface of ilium Greater trochanter of femur, lateral surface Abduction and medial rotation of thigh; steadies the pelvis during walking Superior gluteal Piriformis Anterior surface of sacrum Greater trochanter of femur Abducts thigh Twigs from sacral one and two 31 Quadratus femoris Ischial tuberosity Elevation superior to mid-portion of intertrochanteric crest on posterior femur Laterally rotates and stabilizes hip joint Nerve to quadratus lumborum Psoas Transverse processes of bodies of lumbar vertebrae Lesser trochanter of femur with iliacus Flex thigh at hip joint, rotate thigh laterally, and flex trunk at hip as in sitting up from supine position Femoral Iliac fossa and lateral margin of sacrum Lesser trochanter of femur with psoas major Flex thigh at hip joint, rotate thigh laterally, and flex trunk at hip as in sitting up from supine position Femoral Major Iliacus MUSCLES OF THE POSTERIOR THIGH Biceps femoris ORIGIN Long head, ischial tuberosity; Short head, lateral supracondylar ridge of femur INSERTION ACTION NERVE Head of fibula and lateral condyle of tibia Extension of thigh; flexion of leg Sciatic Semimembranosus Ischial tuberosity Medial condyle of tibia Extension of thigh; flexion of leg Sciatic Semitendinosus Ischial tuberosity, Medial condyle of tibia Extension of thigh; flexion of leg Sciatic 32 MUSCLES OF ANTERIOR AND MEDIAL THIGH ORIGIN INSERTION ACTION NERVE Sartorius Anterior superior iliac spine Medial surface of tibia near the knee joint Flexion, abduction, and lateral rotation of thigh; flexion of leg Femoral Tensor fasciae latae Anterior superior iliac spine of ilium Iliotibial tract Flexes thigh, abducts, and medially rotates thigh; steadies trunk on thigh while standing Superior gluteal Quadriceps femoris Anterior inferior iliac spine and upper margin of acetabulum Tibial tuberosity Extension of leg; flexion of thigh Femoral Vastus lateralis Intertrochanteric line and linea aspera of femur Tibial tuberosity Extends leg Femoral Vastus medialis Intertrochanteric line and linea aspera of femur Tibial tuberosity Extends leg Femoral Vastus intermedius Upper shaft of femur Tibial tuberosity Extends leg Femoral Adductor longus Body of pubic bone Middle third of linea aspera Adduction and flexion of thigh Obturator Adductor magnus Inferior ramus of pubic bone; ramus of ischium Linea aspera and adductor tubercle Adduction and flexion of thigh; extension of thigh Obturator & sciatic Medial surface of tibia near the knee joint Adducts thigh at hip joint, medially rotates thigh, and flexes leg at knee joint. Obturator Rectus femoris Ischial tuberosity of ischium Gracilis Body and inferior ramus of pubic bone 33 Pectineus MUSCLES OF THE POSTERIOR LEG Superior ramus of pubis ORIGIN Just inferior to the lesser trochanter INSERTION Adducts and flexes thigh, assists with medial rotation of thigh ACTION Femoral NERVE Tibialis posterior Posterior surfaces of tibia and fibula; interosseous membrane Navicular, with slips to cuneiform; cuboid; metatarsals 2-4 Plantar flexion and inversion of foot Tibial Flexor digitorum longus Posterior surface of tibia Distal phalanges of digits 2-5 Plantar flexion of foot; flexion of toes Tibial Flexor hallucis longus Posterior surface of fibula; interosseous membrane Distal phalanx of great toe Plantar flexion of foot; flexion of great toe Tibial Soleus Head of fibula; posterior surface of fibula; medial aspect of tibia With gastrocnemius into calcaneus via calcaneal tendon Plantar flexion of foot Tibial Gastrocnemius Medial and lateral condyles of femur With soleus into calcaneus via calcaneal tendon Plantar flexion of foot; flexion of the leg Tibial Popliteus Lateral surface of lateral condyle Posterior surface of tibia just below condyles Flexion of leg; medial rotation of leg to unlock knee prior to leg flexion Tibial Plantaris Lateral supracondylar line of femur Posterior calcaneus via calcaneal tendon Assists in plantar flexion of foot and flexion of leg Tibial 34 MUSCLES OF THE ANTEROLATERAL LEG AND DORSUM OF THE FOOT ORIGIN INSERTION ACTION NERVE Tibialis anterior Lateral condyle and lateral surface of tibia and interosseous membrane Base of first cuneiform and first metatarsal Dorsiflexes and inverts foot Deep fibular Extensor hallucis longus Anterior surface of fibula and interosseous membrane Distal phalanx of great toe Dorsiflexes foot and extends great toe Deep fibular Extensor digitorum longus Lateral condyle of tibia; medial surface of fibula & interosseous membrane Middle and distal phalanges of digits 2-5 Dorsiflexes foot and extends toes Deep fibular Fibularis longus Head and lateral surface of fibula First metatarsal and first cuneiform and Eversion of foot Superficial fibular Fibularis brevis Lateral surface of fibula Fifth metatarsal Eversion of foot Superficial fibular Fibularis tertius Inferior third of anterior surface of fibula and interosseous membrane 5th metatarsal Dorsiflexes ankle and aids in eversion of the foot Deep fibular Extensor digitorum brevis Dorsal surface of calcaneus By four tendons into extensor expansion Extends toes Deep fibular Extensor hallucis brevis Medial surface of calcaneus Proximal phalanx of great toe Extends toes Deep fibular 35 MUSCLES OF THE SOLE OF THE FOOT ORIGIN INSERTION ACTION NERVE Abductor hallucis Tuberosity of calcaneus Medial aspect of proximal phalanx of great toe Abducts and flexes great toe; supports medial longitudinal arch Medial plantar Flexor digitorum brevis Tuberosity of calcaneus By four tendons into middle phalanx of lateral four toes Flexes lateral four toes Medial plantar Abductor digiti minimi Tuberosisty of calcaneus Lateral side of proximal 5th phalanx Abducts 5th Toe Lateral plantar Flexor hallucis brevis Cuboid and lateral cuneiform bones Base of proximal phalanx of 1st toe Flexes great toe Medial plantar Flexor digiti minimi brevis Base of 5th Metatarsal Base of proximal 5th phalanx Flexes 5th toe Lateral plantar The final practical will cover all the material discussed in the last 4 weeks of lab (bones and muscles) Terms to know, labeled figures, tables, any additional info outlined in the package Models Images 75 questions Timed stations 36