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Mutations Review Sheet True or False: 1. TRUE Point mutations affect just one nucleotide. 2. FALSE The substitution of one nucleotide for another in a gene never affects the function of the protein. 3. TRUE Frameshift mutations affect every amino acid that follows the point of the mutation. 4. FALSE All mutations are harmful. 5. FALSE The only source of mutations are errors made during replication. 6. TRUE A mutation is any change in the nucleotide sequence. 7. TRUE Mutations are A source of variety in a population. 8. TRUE The codon sequence is also referred to as the ‘reading frame.’ Complete the following: (you will need to refer to a genetic code (codon table.) 9. Translate the following mRNA: AUG CCA GAC AAU AUU AAG UGA Amino Acids: Methionine- Proline- Aspartic Acid- Asparagine- Isoleucine- Lysine- Stop 10. Translate the following mRNA: AUG CCA GAC CAU AUU AAG UGA Amino Acids: : Methionine- Proline- Aspartic Acid- Histadine- Isoleucine- Lysine- Stop a. How does this mRNA sequence compare to the one in #9? (If it is different describe how it is different). The 4th codon is different- A “C” has been substituted in for an “A” b. How does this amino acid sequence compare to the one in #9? (If it is different describe how it is different) The 4th codon in #9 codes for Asparagine while in #10 the codon codes for Histadine c. What type of mutation is this? The mutation represented is a substitution (point mutation) 11. Translate the following mRNA: AUG CCA GAC GAA UAU UAA GUG A Amino Acids: Methionine- Proline- Aspartic Acid- Glutamic Acid- Tyrosine- Stop On the 4th a. How does this mRNA sequence compare to the one in #9? codon in #11 there is an insertion of a ”G.” b. How does this amino acid sequence compare to the one in #9? c. What type of mutation is this? Frameshift- insertion