Download 26493 Purify Nucleic Acids

NZQA registered unit standard
Title
Purify Nucleic Acids
Level
6
26493 version 2
Page 1 of 4
Credits
4
Purpose
People credited with this unit standard are able to: describe
purification techniques for nucleic acids; and carry out DNA and
RNA purification techniques.
Classification
Science > Molecular Biology
Available grade
Achieved
Entry information
Recommended skills
and knowledge
Unit 8040, Perform aseptic laboratory techniques; Unit 8043,
Perform spectrophotometric analyses; and Unit 8044, Perform
laboratory centrifugation techniques; or demonstrate equivalent
knowledge and skills.
Explanatory notes
1
All work must be carried out in accordance with the quality management system,
documented protocol system or Standard Operating Procedures (SOP) acceptable in
a commercial or research laboratory.
2
Health and Safety practices must conform to Australian/New Zealand Standard
AS/NZS 2243:2006 Set – Safety in Laboratories Parts 1, 2, 3, 7 and 10 available at
http://www.standards.co.nz and http://infostore.saiglobal.com/store.
3
Legislation applicable to this unit standard includes:
Health and Safety in Employment Act 1992;
Hazardous Substances and New Organisms Act 1996.
4
Glossary
Laboratory procedures refer to documented systems or processes of operation which
may be found in a SOP manual, quality management system, or in protocol system
documentation. These procedures are external and/or internal laboratory
requirements governing laboratory work.
NZQA National Qualifications Services
SSB Code 130301
 New Zealand Qualifications Authority 2017
NZQA registered unit standard
26493 version 2
Page 2 of 4
Outcomes and evidence requirements
Outcome 1
Describe purification techniques for nucleic acids.
Range
one each of DNA and RNA.
Evidence requirements
1.1
A technique is selected according to the type of nucleic acid, the material from
which the nucleic acid is to be purified, and quantity required.
1.2
Factors are identified that will influence biochemical properties of the nucleic
acid.
Range
1.3
temperature, concentration, ionic strength, nuclease activity,
shearing effects, storage conditions.
Factors are identified that will influence biochemical properties of DNA and
RNA.
Range
two of – temperature, concentration, ionic strength, shearing
effects, storage conditions.
Outcome 2
Carry out a DNA purification technique.
Range
temperature, concentration, ionic strength, nuclease activity, shearing effects,
storage conditions.
Evidence requirements
2.1
Disruption procedure is selected according to cell or tissue type to allow for
functional integrity of DNA to be maintained.
2.2
DNA is extracted from a sample and purified in accordance with laboratory
procedures.
2.3
DNA yield and purity are determined in accordance with laboratory procedures.
NZQA National Qualifications Services
SSB Code 130301
 New Zealand Qualifications Authority 2017
NZQA registered unit standard
26493 version 2
Page 3 of 4
Outcome 3
Carry out an RNA purification technique.
Range
material from which RNA is to be purified may include two of – plant, animal,
microorganism, electrophoresis gel.
Evidence requirements
3.1
Ribonuclease free conditions are maintained and RNA is obtained in
accordance with laboratory procedures.
3.2
RNA yield and purity are determined in accordance with laboratory procedures.
Replacement information
This unit standard replaced unit standard 8061 and unit
standard 8062.
Planned review date
31 December 2018
Status information and last date for assessment for superseded versions
Process
Version Date
Last Date for Assessment
Registration
1
17 September 2010
N/A
Rollover
2
27 January 2015
N/A
Consent and Moderation Requirements (CMR) reference
0152
This CMR can be accessed at http://www.nzqa.govt.nz/framework/search/index.do.
Please note
Providers must be granted consent to assess against standards (accredited) by NZQA,
before they can report credits from assessment against unit standards or deliver courses
of study leading to that assessment.
Industry Training Organisations must be granted consent to assess against standards by
NZQA before they can register credits from assessment against unit standards.
Providers and Industry Training Organisations, which have been granted consent and
which are assessing against unit standards must engage with the moderation system that
applies to those standards.
Requirements for consent to assess and an outline of the moderation system that applies
to this standard are outlined in the Consent and Moderation Requirements (CMR). The
CMR also includes useful information about special requirements for organisations wishing
to develop education and training programmes, such as minimum qualifications for tutors
and assessors, and special resource requirements.
NZQA National Qualifications Services
SSB Code 130301
 New Zealand Qualifications Authority 2017
NZQA registered unit standard
26493 version 2
Page 4 of 4
Comments on this unit standard
Please contact NZQA National Qualifications Services [email protected] if you wish to
suggest changes to the content of this unit standard.
NZQA National Qualifications Services
SSB Code 130301
 New Zealand Qualifications Authority 2017