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NZQA registered unit standard Title Purify Nucleic Acids Level 6 26493 version 2 Page 1 of 4 Credits 4 Purpose People credited with this unit standard are able to: describe purification techniques for nucleic acids; and carry out DNA and RNA purification techniques. Classification Science > Molecular Biology Available grade Achieved Entry information Recommended skills and knowledge Unit 8040, Perform aseptic laboratory techniques; Unit 8043, Perform spectrophotometric analyses; and Unit 8044, Perform laboratory centrifugation techniques; or demonstrate equivalent knowledge and skills. Explanatory notes 1 All work must be carried out in accordance with the quality management system, documented protocol system or Standard Operating Procedures (SOP) acceptable in a commercial or research laboratory. 2 Health and Safety practices must conform to Australian/New Zealand Standard AS/NZS 2243:2006 Set – Safety in Laboratories Parts 1, 2, 3, 7 and 10 available at http://www.standards.co.nz and http://infostore.saiglobal.com/store. 3 Legislation applicable to this unit standard includes: Health and Safety in Employment Act 1992; Hazardous Substances and New Organisms Act 1996. 4 Glossary Laboratory procedures refer to documented systems or processes of operation which may be found in a SOP manual, quality management system, or in protocol system documentation. These procedures are external and/or internal laboratory requirements governing laboratory work. NZQA National Qualifications Services SSB Code 130301 New Zealand Qualifications Authority 2017 NZQA registered unit standard 26493 version 2 Page 2 of 4 Outcomes and evidence requirements Outcome 1 Describe purification techniques for nucleic acids. Range one each of DNA and RNA. Evidence requirements 1.1 A technique is selected according to the type of nucleic acid, the material from which the nucleic acid is to be purified, and quantity required. 1.2 Factors are identified that will influence biochemical properties of the nucleic acid. Range 1.3 temperature, concentration, ionic strength, nuclease activity, shearing effects, storage conditions. Factors are identified that will influence biochemical properties of DNA and RNA. Range two of – temperature, concentration, ionic strength, shearing effects, storage conditions. Outcome 2 Carry out a DNA purification technique. Range temperature, concentration, ionic strength, nuclease activity, shearing effects, storage conditions. Evidence requirements 2.1 Disruption procedure is selected according to cell or tissue type to allow for functional integrity of DNA to be maintained. 2.2 DNA is extracted from a sample and purified in accordance with laboratory procedures. 2.3 DNA yield and purity are determined in accordance with laboratory procedures. NZQA National Qualifications Services SSB Code 130301 New Zealand Qualifications Authority 2017 NZQA registered unit standard 26493 version 2 Page 3 of 4 Outcome 3 Carry out an RNA purification technique. Range material from which RNA is to be purified may include two of – plant, animal, microorganism, electrophoresis gel. Evidence requirements 3.1 Ribonuclease free conditions are maintained and RNA is obtained in accordance with laboratory procedures. 3.2 RNA yield and purity are determined in accordance with laboratory procedures. Replacement information This unit standard replaced unit standard 8061 and unit standard 8062. Planned review date 31 December 2018 Status information and last date for assessment for superseded versions Process Version Date Last Date for Assessment Registration 1 17 September 2010 N/A Rollover 2 27 January 2015 N/A Consent and Moderation Requirements (CMR) reference 0152 This CMR can be accessed at http://www.nzqa.govt.nz/framework/search/index.do. Please note Providers must be granted consent to assess against standards (accredited) by NZQA, before they can report credits from assessment against unit standards or deliver courses of study leading to that assessment. Industry Training Organisations must be granted consent to assess against standards by NZQA before they can register credits from assessment against unit standards. Providers and Industry Training Organisations, which have been granted consent and which are assessing against unit standards must engage with the moderation system that applies to those standards. Requirements for consent to assess and an outline of the moderation system that applies to this standard are outlined in the Consent and Moderation Requirements (CMR). The CMR also includes useful information about special requirements for organisations wishing to develop education and training programmes, such as minimum qualifications for tutors and assessors, and special resource requirements. NZQA National Qualifications Services SSB Code 130301 New Zealand Qualifications Authority 2017 NZQA registered unit standard 26493 version 2 Page 4 of 4 Comments on this unit standard Please contact NZQA National Qualifications Services [email protected] if you wish to suggest changes to the content of this unit standard. NZQA National Qualifications Services SSB Code 130301 New Zealand Qualifications Authority 2017