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SYBR Green I Nucleic Acid Gel Stain
SYBR Green I Nucleic Acid Gel Stain

background of the invention
background of the invention

... The present invention relates to a biosensor and a bio-signal amplification method of the biosensor, in particular to a field-effect transistor type biosensor and a bio-signal amplification method thereof that integrate an isothermal nucleic acid amplification technology Description of the Related A ...
Lab 22 DNA-based Screening for Smallpox as a Bio
Lab 22 DNA-based Screening for Smallpox as a Bio

Prediction and investigation of novel proteins in DNA double
Prediction and investigation of novel proteins in DNA double

... ligation of the broken ends of DNA. The process of NHEJ involves three main protein complexes: Yku70/Yku80 initiates and stabilizes the DNA ends, Mre11/Rad50/Xrs2 brings broken ends to close proximity and Dnl4/Lif1/Nej1 ligates the DNA ends. Protein-protein interaction (PPI) has been utilized in fun ...
What are rare chromosome disorders?
What are rare chromosome disorders?

... cell contains a NUCLEUS. Inside the nucleus are the CHROMOSOMES, which contain GENES • We each usually have 23 pairs of chromosomes in every cell • Chromosome 1 is the largest, with 22 the smallest. The 23rd pair are the sex chromosomes (males = XY, females = XX) ...


... concentration and the binding curves are shown on the right. Based on these data, what type(s) of interactions are used by the lac repressor to bind to DNA [Hint: It may be useful to sketch a plot of KD versus [NaCl]] The Kd increases as salt increases, indicating weaker binding (3 1/2 pts). The int ...
CHAPTER 17 Variation in Chromosomal Number and Structure
CHAPTER 17 Variation in Chromosomal Number and Structure

... iv.Transposable elements. v. Errors in recombination. b. Deletions do not revert, because the DNA is missing. ...
CHAPTER 21 Chromosomal Mutations
CHAPTER 21 Chromosomal Mutations

... iv.Transposable elements. v. Errors in recombination. b. Deletions do not revert, because the DNA is missing. ...
Regulation of Bacterial Conjugation in Microaerobiosis by
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... screens for host-encoded activators and repressors of the transfer operon (tra) of pSLT. We show that the transcription factor ArcA is an activator of conjugation, especially under microaerobiosis. In turn, succinate dehydrogenase (SdhABCD) is a repressor of mating in aerobiosis. ArcA binds upstream ...
Distinguishing Different DNA Heterozygotes by
Distinguishing Different DNA Heterozygotes by

... However, it was not clear whether different heterozygotes within the same amplicon could be distinguished from each other based on curve shape differences. Four different classes of SNPs have been defined based on the homo- and heteroduplexes that are produced after amplification (1 ). Because the h ...
Inhibition of RNA Synthesis by Anthracycline Analogs
Inhibition of RNA Synthesis by Anthracycline Analogs

Localization and structural analysis of the ribosomal RNA operons of
Localization and structural analysis of the ribosomal RNA operons of

Bacteriophage A cloning system for the construction of
Bacteriophage A cloning system for the construction of

... We have developed a bacteriophage A cloning vector, XORF8, that can be used for the construction of cDNA libraries. The wild-type A genome contains five BamHI, five EcoRI, and seven Hindu restriction sites that have all been removed from the genome of AORF8. Sites for these endonucleases are present ...
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DNA METHODS FOR HLA TYPING A WORKBOOK FOR - ASHI-U
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The Use of Cytochrome B Sequence Variation in Estimation of

Microplate-Based Pathlength Correction Method for Photometric
Microplate-Based Pathlength Correction Method for Photometric

... 0.3–0.5 µg/ml of DNA with a 384-well plate, and between 0.5–0.9 µg/ml with a 96-well plate. This is the lowest concentration that can be reliably separated from the blank level in order to determine whether a sample contains DNA or not. For reliable numeric concentration values one must have at leas ...
The msh2 Gene of Schizosaccharomyces pombe Is
The msh2 Gene of Schizosaccharomyces pombe Is

... homology to the Msh2 subgroup. msh2 disruption gives rise to increased mitotic mutation rates and increased levels of postmeiotic segregation of genetic markers. In bandshift assays performed with msh2D cell extracts, a general mismatch-binding activity is absent. By complementation assays, we showe ...
transposon
transposon

... is given the prefix IS, followed by a number that identifies the type.  The IS elements are normal constituents of bacterial chromosomes and plasmids. To describe an insertion into a particular site, a double colon is used; so λ::IS1 describes an IS1 element inserted into phage lambda.  The IS ele ...
CBSE XII BIO QS with key(2009-2015)
CBSE XII BIO QS with key(2009-2015)

... they have food reserves for the seedling or embryo the seed coat provides protection to the embryo generate genetic variation remain viable for a considerable period of time.(any two) =1x2 State what is apomixis? Comment on its significance. How can it be commercially used? (2015) Form of asexual re ...
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Structural and Functional Studies of Insertion Element IS200
Structural and Functional Studies of Insertion Element IS200

Molecular Biology and Applied Genetics
Molecular Biology and Applied Genetics

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Taxonomic characterization of Ochrobactrum sp. isolates from soil
Taxonomic characterization of Ochrobactrum sp. isolates from soil

... wheat rhizoplane, was subjected to polyphasic taxonomy by examining various pheno- and genotypic parameters. Strains were grouped on (inter) repetitive extragenic palindromic DNA (REP) PCR profiles at the intraspecies level. Pheno- and genotypic characters were assessed for representatives from 13 d ...
An assessment of factors affecting the likelihood
An assessment of factors affecting the likelihood

... naturally released in situ e.g. in soil or in planta, has not been shown. Information on the general composition, dynamics and succession of bacterial communities in the phytosphere of various agriculturally grown plants needs to be further determined and understood before the potential bacterial re ...
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DNA supercoil



DNA supercoiling refers to the over- or under-winding of a DNA strand, and is an expression of the strain on that strand. Supercoiling is important in a number of biological processes, such as compacting DNA. Additionally, certain enzymes such as topoisomerases are able to change DNA topology to facilitate functions such as DNA replication or transcription. Mathematical expressions are used to describe supercoiling by comparing different coiled states to relaxed B-form DNA.As a general rule, the DNA of most organisms is negatively supercoiled.
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