Telomere maintenance without telomerase
... Discovery of a telomerase-independent pathway for telomere maintenance Several observations during the 1980's provided early clues that recombination could potentially maintain telomeric repeats at chromosome ends. Linear plasmids that lacked a functional telomere, but contained homology to sub-telo ...
... Discovery of a telomerase-independent pathway for telomere maintenance Several observations during the 1980's provided early clues that recombination could potentially maintain telomeric repeats at chromosome ends. Linear plasmids that lacked a functional telomere, but contained homology to sub-telo ...
The sequence of human serum albumin cDNA and its expression in
... corresponding to amino acid codons no. 175-179). Although not employed in the construction of the mature HSA expression plasmid, this cDNA clone (P-14) allowed determination of the DNA sequence of the "prepro" peptide coding and 5' non-coding regions of HSA mRNA. The mature HSA mRNA sequence was joi ...
... corresponding to amino acid codons no. 175-179). Although not employed in the construction of the mature HSA expression plasmid, this cDNA clone (P-14) allowed determination of the DNA sequence of the "prepro" peptide coding and 5' non-coding regions of HSA mRNA. The mature HSA mRNA sequence was joi ...
IOSR Journal of Dental and Medical Sciences (IOSR-JDMS)
... Certain previously described CCRs (Table 1) in female cases were also ascertained by recurrent miscarriages were all phenotypically normal, it is likely that these chromosomal breakpoints might not include genes or gene regulatory regions whose disruptions may give rise to physical dysfunction and c ...
... Certain previously described CCRs (Table 1) in female cases were also ascertained by recurrent miscarriages were all phenotypically normal, it is likely that these chromosomal breakpoints might not include genes or gene regulatory regions whose disruptions may give rise to physical dysfunction and c ...
Methods of Human Heredity Study
... Besides centromere, which produces a primary constriction in chromosomes, secondary constrictions can also be observed in some chromosomes. Such a secondary constriction if presents in the distal region of an arm would pinch off a small fragment called trabant or satellite (Fig.3). The satellite re ...
... Besides centromere, which produces a primary constriction in chromosomes, secondary constrictions can also be observed in some chromosomes. Such a secondary constriction if presents in the distal region of an arm would pinch off a small fragment called trabant or satellite (Fig.3). The satellite re ...
53 - Lab Times
... ligation steps are rather time consuming. That’s usually no problem for researchers wanting to clone single genes. However, this species of molecular biologist seems to be in danger of extinction when it comes to sequencing entire genomes like there’s no tomorrow. Instead of cloning single genes, as ...
... ligation steps are rather time consuming. That’s usually no problem for researchers wanting to clone single genes. However, this species of molecular biologist seems to be in danger of extinction when it comes to sequencing entire genomes like there’s no tomorrow. Instead of cloning single genes, as ...
Myriad--Ambry -- Final Version of Ambry Preliminary Injunction
... Chromosomes in a human cell are made up of two complementary strands of DNA molecules—one strand is on one side of the double helix and the second strand is on the other side. For any given gene, only one molecule strand (the “template strand”) is actually transcribed into mRNA and ultimately used t ...
... Chromosomes in a human cell are made up of two complementary strands of DNA molecules—one strand is on one side of the double helix and the second strand is on the other side. For any given gene, only one molecule strand (the “template strand”) is actually transcribed into mRNA and ultimately used t ...
An organism containing a normal chromosome complement and
... Other aneuploids (i.e. primary trisomics, tetrasomics, multiple trisomics, secondary trisomics, tertiary trisomics, compensating trisomics) ...
... Other aneuploids (i.e. primary trisomics, tetrasomics, multiple trisomics, secondary trisomics, tertiary trisomics, compensating trisomics) ...
ANALYSIS OF MULTIPLE RESTRICTION FRAGMENT LENGTH
... using CRI-4 as the probe. In three of the four individuals having the S allotype, a 14 .5-kb fragment was identified that was absent in individuals homozygous for the F allotype (Fig . 2A). We could find no fragment present in individuals having the F allotype that was absent in the SS individuals, ...
... using CRI-4 as the probe. In three of the four individuals having the S allotype, a 14 .5-kb fragment was identified that was absent in individuals homozygous for the F allotype (Fig . 2A). We could find no fragment present in individuals having the F allotype that was absent in the SS individuals, ...
DNA cytosine methylation in plant development
... of CG methylation that reside predominantly at the 3′ end of genes; nonetheless, like gene-body methylation in general, the genesis and biological function of methylation in these regions are also largely unknown (Tran et al., 2005). Such detailed cytosine methylomes remain to be established for oth ...
... of CG methylation that reside predominantly at the 3′ end of genes; nonetheless, like gene-body methylation in general, the genesis and biological function of methylation in these regions are also largely unknown (Tran et al., 2005). Such detailed cytosine methylomes remain to be established for oth ...
Assessment of the mosaic structure in the
... Numerous PCR amplification assays have been described for the identification of the CagA EPIYA/T phosphorylation motif, including multiple PCR amplification steps and ethidium-bromide stained agarose gel electrophoresis (summarised in ref [18]). Commonly, amplicons are sequenced using a battery of g ...
... Numerous PCR amplification assays have been described for the identification of the CagA EPIYA/T phosphorylation motif, including multiple PCR amplification steps and ethidium-bromide stained agarose gel electrophoresis (summarised in ref [18]). Commonly, amplicons are sequenced using a battery of g ...
Practice exam 3 key
... (3 pts) Leaves single-stranded overhangs (or 'sticky ends') (1 pt); these ends are complementary (will hybridize) (1 pt); can be used to join 2 DNA fragments cut with EcoRI (with the same restriction enzyme) (1 pt). ...
... (3 pts) Leaves single-stranded overhangs (or 'sticky ends') (1 pt); these ends are complementary (will hybridize) (1 pt); can be used to join 2 DNA fragments cut with EcoRI (with the same restriction enzyme) (1 pt). ...
Chromosomal Basis of Inherited Disorders
... cells derived from that one will have the same inactive X chromosome or Barr body. By this process, females compensate for their double genetic dose of X chromosome. In so-called tortoiseshell cats, embryonic X inactivation is observed as color variegation (Figure 5). Females that are heterozygous ...
... cells derived from that one will have the same inactive X chromosome or Barr body. By this process, females compensate for their double genetic dose of X chromosome. In so-called tortoiseshell cats, embryonic X inactivation is observed as color variegation (Figure 5). Females that are heterozygous ...
Multiple Roles of the Y Chromosome in the Biology of
... segregates from the X chromosome, irrespective of its origin”[15]. This suggests two considerations: (1) although all Y chromosomes seem to evolve similarly in many organisms once they have been established, their origin may be very different even in the same taxon[15,38], being derived either from ...
... segregates from the X chromosome, irrespective of its origin”[15]. This suggests two considerations: (1) although all Y chromosomes seem to evolve similarly in many organisms once they have been established, their origin may be very different even in the same taxon[15,38], being derived either from ...
Mosaic Isodicentric Y Chromosome in a Patient with Mixed Gonadal
... chromosome Y probe revealed that the derivative chromosome is a Y chromosome, without translocation a heterochromatic region on Yq was not observed by CBG banding. FISH examination by probes for the SRY region and whole chromosome Y revealed that the derivative chromosome is a Y chromosome, and one ...
... chromosome Y probe revealed that the derivative chromosome is a Y chromosome, without translocation a heterochromatic region on Yq was not observed by CBG banding. FISH examination by probes for the SRY region and whole chromosome Y revealed that the derivative chromosome is a Y chromosome, and one ...
Variation in the size of the oqhcontaining linear
... Species variation in the size of the ospA-containing plasmid The size of the ospA-containing linear DNA molecule, the largest of the plasmids in most isolates, was determined by constant-field electrophoresis in 0.2 YO agarose gels (a representative gel is shown in Fig. la), which have a linear rang ...
... Species variation in the size of the ospA-containing plasmid The size of the ospA-containing linear DNA molecule, the largest of the plasmids in most isolates, was determined by constant-field electrophoresis in 0.2 YO agarose gels (a representative gel is shown in Fig. la), which have a linear rang ...
ARTICLES - Weizmann Institute of Science
... expressed genes. Consistent with this expectation, the highly expressed ribosomal RNA and transfer RNA genes stood out as having markedly low predicted nucleosome occupancy. In contrast to the ubiquitously expressed tRNAs, many other genes vary their expression between high and low levels in differe ...
... expressed genes. Consistent with this expectation, the highly expressed ribosomal RNA and transfer RNA genes stood out as having markedly low predicted nucleosome occupancy. In contrast to the ubiquitously expressed tRNAs, many other genes vary their expression between high and low levels in differe ...
video slide - Dublin City Schools Home
... • While transgenic animals are currently used to produce potentially useful proteins, none are yet found in our food supply. • It is possible that DNA technology will eventually replace traditional animal breeding. ...
... • While transgenic animals are currently used to produce potentially useful proteins, none are yet found in our food supply. • It is possible that DNA technology will eventually replace traditional animal breeding. ...
Structure and function of the GINS complex, a key component of the
... conditions from exponentially growing yeast cells and was shown to co-purify with Sld5 and Psf3, as well as with a novel 213-amino-acid protein later designated Psf2 [5]. Overproduction of PSF2, like PSF1, was also shown to be able to suppress to sld5-12. Further biochemical analysis showed that all ...
... conditions from exponentially growing yeast cells and was shown to co-purify with Sld5 and Psf3, as well as with a novel 213-amino-acid protein later designated Psf2 [5]. Overproduction of PSF2, like PSF1, was also shown to be able to suppress to sld5-12. Further biochemical analysis showed that all ...
Structural organization of the transfer RNA gene clusters of cholera
... most cases, host aminoacyl-tRNA synthetases and tRNAs. However, infections of Escherichia coli cells with T5 (Scherberg and Weiss 1970) and T-even phages (McClain et al 1972; Wilson et al 1972; Desai and Weiss 1977) induce the synthesis of a large number of tRNA species coded by the phage genome. A ...
... most cases, host aminoacyl-tRNA synthetases and tRNAs. However, infections of Escherichia coli cells with T5 (Scherberg and Weiss 1970) and T-even phages (McClain et al 1972; Wilson et al 1972; Desai and Weiss 1977) induce the synthesis of a large number of tRNA species coded by the phage genome. A ...
DNA supercoil
DNA supercoiling refers to the over- or under-winding of a DNA strand, and is an expression of the strain on that strand. Supercoiling is important in a number of biological processes, such as compacting DNA. Additionally, certain enzymes such as topoisomerases are able to change DNA topology to facilitate functions such as DNA replication or transcription. Mathematical expressions are used to describe supercoiling by comparing different coiled states to relaxed B-form DNA.As a general rule, the DNA of most organisms is negatively supercoiled.