Ribotyping of Clostridium perfringens from industrially produced
... ribopattern. Figure 1 shows an example of the variability of the ribotype patterns. The number of DIG labelled bands of Cl. perfringens varied between 12 and 17. Typical ribotype patterns of Cl. perfringens were as follows: 7–11 bands between 23 kb and 4Æ3 kb, 0–4 bands between 4Æ3 kb and 2Æ0 kb and ...
... ribopattern. Figure 1 shows an example of the variability of the ribotype patterns. The number of DIG labelled bands of Cl. perfringens varied between 12 and 17. Typical ribotype patterns of Cl. perfringens were as follows: 7–11 bands between 23 kb and 4Æ3 kb, 0–4 bands between 4Æ3 kb and 2Æ0 kb and ...
Nucleic Acids - Farmasi Unand
... • However, other groups in the structures may also contribute to the binding of a drug to the DNA. • For example, the amino group of the sugar residue of doxorubicin forms an ionic bond with the negatively charged oxygens of the phosphate groups of the DNA chain, which effectively locks the drug in ...
... • However, other groups in the structures may also contribute to the binding of a drug to the DNA. • For example, the amino group of the sugar residue of doxorubicin forms an ionic bond with the negatively charged oxygens of the phosphate groups of the DNA chain, which effectively locks the drug in ...
Synthesis of Oligonucleotides
... Nucleobases. Permanent protecting groups for the exocyclic amino groups of adenine, cytosine and guanine have been used for many years in oligonucleotide synthesis.1 Acyl protecting groups were chosen, since they are stable for long periods during mildly basic and acidic conditions used during oligo ...
... Nucleobases. Permanent protecting groups for the exocyclic amino groups of adenine, cytosine and guanine have been used for many years in oligonucleotide synthesis.1 Acyl protecting groups were chosen, since they are stable for long periods during mildly basic and acidic conditions used during oligo ...
Glycolysis Lecture
... no reason to suppose that Biochemistry is intrinsically uninteresting, difficult to understand or an obstacle to be overcome during your progress to a professional qualifications. In the contrary you can enjoy its study if you remember the simple biological principals that the body is formed from or ...
... no reason to suppose that Biochemistry is intrinsically uninteresting, difficult to understand or an obstacle to be overcome during your progress to a professional qualifications. In the contrary you can enjoy its study if you remember the simple biological principals that the body is formed from or ...
Engineered Cpf1 Enzymes with Altered PAM Specificities
... Previous work has shown that the PAM preference of Cas9 can be altered by mutations to residues in close proximity with the PAM DNA duplex6-9. We sought to investigate whether the PAM preference of Cpf1, despite its strong evolutionary conservation1, can also be modified. Based on the crystal struct ...
... Previous work has shown that the PAM preference of Cas9 can be altered by mutations to residues in close proximity with the PAM DNA duplex6-9. We sought to investigate whether the PAM preference of Cpf1, despite its strong evolutionary conservation1, can also be modified. Based on the crystal struct ...
Forensic Science Handbook
... scientifically validated procedures, and quality-control policies that are enforced for the benefit of your agency. They are in place to protect the integrity of the laboratory’s work product and to prevent complications to ongoing investigations and court proceedings resulting from shoddy laborator ...
... scientifically validated procedures, and quality-control policies that are enforced for the benefit of your agency. They are in place to protect the integrity of the laboratory’s work product and to prevent complications to ongoing investigations and court proceedings resulting from shoddy laborator ...
Identifikasi Molekular Bakteri Pathogen yang Menginfeksi Hama
... of the entomophatogens are essential for development of biological control agent to the pest. Brontispa longissima, a serious and invasive pest of coconut, was infected by bacterium causing mortality of the larvae and pupae in coconut field. Objective of the research was to identify bacterium as a c ...
... of the entomophatogens are essential for development of biological control agent to the pest. Brontispa longissima, a serious and invasive pest of coconut, was infected by bacterium causing mortality of the larvae and pupae in coconut field. Objective of the research was to identify bacterium as a c ...
fulltext
... Tardigrades are known for being resistant to extreme conditions, including tolerance to ionising and UV radiation in both the hydrated and the dehydrated state. It is known that these factors may cause damage to DNA. It has recently been shown that single and double DNA strand breaks occur when tard ...
... Tardigrades are known for being resistant to extreme conditions, including tolerance to ionising and UV radiation in both the hydrated and the dehydrated state. It is known that these factors may cause damage to DNA. It has recently been shown that single and double DNA strand breaks occur when tard ...
Maintenance of genomic integrity by p53: complementary
... themselves, and the role p53 plays in their activation are far less clear. Furthermore, it is still not known, whether and how p53 directly participates in DNA repair processes, despite some evidence pointing to this possibility. Last but not least, a possible role of p53 in the control of genomic i ...
... themselves, and the role p53 plays in their activation are far less clear. Furthermore, it is still not known, whether and how p53 directly participates in DNA repair processes, despite some evidence pointing to this possibility. Last but not least, a possible role of p53 in the control of genomic i ...
Eds., N. Hamamura, S. Suzuki, S. Mendo, C. M. Barroso,... © by TERRAPUB, 2010.
... PCR was carried out in 50 µl reaction mixture consisting of 3.0 mM MgCl2, 0.3 pmol of each oligonucleotide (Table 1), 0.2 mM of each dATP, dCTP, dGTP, and dTTP, 1 × green GoTaq®Flexi Buffer (Promega, USA), 1 U GoTaq® DNA polymerase (Promega, USA), 10–100 ng plasmid DNA of clone 69. Twenty-five ampli ...
... PCR was carried out in 50 µl reaction mixture consisting of 3.0 mM MgCl2, 0.3 pmol of each oligonucleotide (Table 1), 0.2 mM of each dATP, dCTP, dGTP, and dTTP, 1 × green GoTaq®Flexi Buffer (Promega, USA), 1 U GoTaq® DNA polymerase (Promega, USA), 10–100 ng plasmid DNA of clone 69. Twenty-five ampli ...
rec-mediated recombinational hot spot activity in bacteriophage
... are termed “hot spot survey crosses.” These crosses are carried out using conditions which block nearly all DNA synthesis (MCMILINand Russo 1972). Though there is some residual DNA synthesis, which is potentially very interesting (J. SIEGEL,nianuscript in preparation), it is convenient and justifiab ...
... are termed “hot spot survey crosses.” These crosses are carried out using conditions which block nearly all DNA synthesis (MCMILINand Russo 1972). Though there is some residual DNA synthesis, which is potentially very interesting (J. SIEGEL,nianuscript in preparation), it is convenient and justifiab ...
arXiv:0708.2724v1 [cond-mat.other] 20 Aug 2007
... There is also a global property called secondary structure. For ds-DNA, for instance, there exist several different types of helices. The most common ones are called A-DNA and B-DNA. What changes the global structure of DNA between A- and B-DNA is the ionic and water environment. B-DNA is preferred ...
... There is also a global property called secondary structure. For ds-DNA, for instance, there exist several different types of helices. The most common ones are called A-DNA and B-DNA. What changes the global structure of DNA between A- and B-DNA is the ionic and water environment. B-DNA is preferred ...
paper - Université de Namur
... understand the X-ray images of DNA. However the use of the CCV theory for its originally intended purpose [3], namely for the interpretation of X-ray diffraction by protein α-helices, is a bit too advanced and will not be pursued in the present didactic paper (a recent review [4] covers this and fur ...
... understand the X-ray images of DNA. However the use of the CCV theory for its originally intended purpose [3], namely for the interpretation of X-ray diffraction by protein α-helices, is a bit too advanced and will not be pursued in the present didactic paper (a recent review [4] covers this and fur ...
transposon
... is given the prefix IS, followed by a number that identifies the type. The IS elements are normal constituents of bacterial chromosomes and plasmids. To describe an insertion into a particular site, a double colon is used; so λ::IS1 describes an IS1 element inserted into phage lambda. The IS ele ...
... is given the prefix IS, followed by a number that identifies the type. The IS elements are normal constituents of bacterial chromosomes and plasmids. To describe an insertion into a particular site, a double colon is used; so λ::IS1 describes an IS1 element inserted into phage lambda. The IS ele ...
GENtle, a free multi-purpose molecular biology tool
... design, alignment generation and layout, chromatogram and image display, as well as many related functions. GENtle strives to satisfy the need for an easy and comfortable, ...
... design, alignment generation and layout, chromatogram and image display, as well as many related functions. GENtle strives to satisfy the need for an easy and comfortable, ...
video slide - Dublin City Schools Home
... • While transgenic animals are currently used to produce potentially useful proteins, none are yet found in our food supply. • It is possible that DNA technology will eventually replace traditional animal breeding. ...
... • While transgenic animals are currently used to produce potentially useful proteins, none are yet found in our food supply. • It is possible that DNA technology will eventually replace traditional animal breeding. ...
The effecT of chlorinaTion of nucleoTide bases on The
... Fig. 4. The structure of the pairs of a conformers 42 of 5'-thymidylic acid (a) and 5-chloro-2'-deoxyuridine monophosphate (b) molecules where the cause of conformational parameters changes is turning of the phosphate group due to the formation and / or rupture of C7-H72···Oр аnd Op1-Hp1···Cl bonds ...
... Fig. 4. The structure of the pairs of a conformers 42 of 5'-thymidylic acid (a) and 5-chloro-2'-deoxyuridine monophosphate (b) molecules where the cause of conformational parameters changes is turning of the phosphate group due to the formation and / or rupture of C7-H72···Oр аnd Op1-Hp1···Cl bonds ...
File
... One distinction needs to be made at the outset. Throughout this text, various molecular processes are described as occurring in "bacteria." The "bacteria" to which I refer are the common bacteria (formerly called eubacteria). The archaea or archaebacteria, including the thermophilic (heatloving), ex ...
... One distinction needs to be made at the outset. Throughout this text, various molecular processes are described as occurring in "bacteria." The "bacteria" to which I refer are the common bacteria (formerly called eubacteria). The archaea or archaebacteria, including the thermophilic (heatloving), ex ...
The Art of Multiple Sequence Alignment in R - decipher
... This document is intended to illustrate the art of multiple sequence alignment in R using DECIPHER. Even though its beauty is often concealed, multiple sequence alignment is a form of art in more ways than one. Take a look at Figure 1 for an illustration of what is happening behind the scenes during ...
... This document is intended to illustrate the art of multiple sequence alignment in R using DECIPHER. Even though its beauty is often concealed, multiple sequence alignment is a form of art in more ways than one. Take a look at Figure 1 for an illustration of what is happening behind the scenes during ...
... or van der Waals, plays an important role in the stability of double stranded DNA, but not protein stability. Why is this interaction more important for DNA? Electrostatic interactions (not H-bonds, these are also important in stabilizing the folded protein). The phosphate groups on DNA (and RNA) ar ...
Quantitative Analysis of the Kinetics of End
... RecA protein will form mixed ®laments on DNA with the RecA K72R mutant protein that binds but does not hydrolyze ATP or dATP (Shan & Cox, 1996). In most experiments with the mutant, dATP replaces ATP since the mutant protein functions in some RecA assays only in the presence of dATP (Rehrauer & Kowa ...
... RecA protein will form mixed ®laments on DNA with the RecA K72R mutant protein that binds but does not hydrolyze ATP or dATP (Shan & Cox, 1996). In most experiments with the mutant, dATP replaces ATP since the mutant protein functions in some RecA assays only in the presence of dATP (Rehrauer & Kowa ...
DNA supercoil
DNA supercoiling refers to the over- or under-winding of a DNA strand, and is an expression of the strain on that strand. Supercoiling is important in a number of biological processes, such as compacting DNA. Additionally, certain enzymes such as topoisomerases are able to change DNA topology to facilitate functions such as DNA replication or transcription. Mathematical expressions are used to describe supercoiling by comparing different coiled states to relaxed B-form DNA.As a general rule, the DNA of most organisms is negatively supercoiled.