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A novel gene encoding a 54 kDa polypeptide is
A novel gene encoding a 54 kDa polypeptide is

... bacteria, and selection, being a growth-dependent process, may miss out organisms which require different media or temperatures. Polyclonal and monoclonal antibodies have proved to be more reliable and easy to use for detection of target organisms. However, this method of detection depends on the pr ...
Determination of guanine-plus-cytosine content of
Determination of guanine-plus-cytosine content of

... A dual-laser flow cytometer was used to analyse different species of bacteria for the molar percentage of guanineplus-cytosine (% G + C) without the need for DNA extraction or purification. Ethanol-fixed bacterial cells were stained with a combination of DNA-specific fluorochromes, Hoechst 33258 and ...
BIOLOGY (Theory) 57/2 SECTION – A 1. Name the two gases
BIOLOGY (Theory) 57/2 SECTION – A 1. Name the two gases

... 7. State one reason why breast-feeding the baby acts as a natural contraceptive for the mother. Ans. Breast-feeding prevents ovulation during lactation/absence of menstruation ...
Chapter 13 Chromosomes
Chapter 13 Chromosomes

... euploid = correct chromosome # aneuploid = missing or extra chromosome polyploidy = ...
ANSWERS TO REVIEW QUESTIONS
ANSWERS TO REVIEW QUESTIONS

... b. A female with XO Turner syndrome has wide-set nipples, flaps of skin on the neck, and no secondary sexual development. c. A female with trisomy 21 Down syndrome. Phenotype includes short, sparse, straight hair, wide-set eyes with epicanthal folds, a broad nose, protruding tongue, mental retardati ...
Molecular cloning and nucleotide sequence of another variant of the
Molecular cloning and nucleotide sequence of another variant of the

... Nucleotide sequence of E. coli SLTIIva genes membranes were given three 10 min washes with a solution of 2 x SSC/O.l%SDS at 42 "C and two 15 min washes with a solution of 0.1 x SSC/O.l% SDS at 65 "C. Membranes for hybridization with oligonucleotide probes were prehybridized in 6 x SSC, 5 x Denhardt ...
A Rapid Screening Method to Detect Nonsense and Frameshift
A Rapid Screening Method to Detect Nonsense and Frameshift

... mutations and are not applicable for detection of unknown mutations (8, 15). In APC screening, for example, these methods would be inefficient, as none of the mutations identified to date has been found at a frequency of more than 1 in 10. A functional assay for the detection of p53 mutations in Li- ...
RHD - Labex
RHD - Labex

... But - exons 7 and 10 are not suitable for testing any population containing people of African origin, as they will give false-positive results when the fetus has RHDΨ ...
Classification of nucleic acids structures by means of the
Classification of nucleic acids structures by means of the

... Now, we will study in detail each one of these two main branches. The left one contains in turn three clusters. The first one (samples 2 to 16) undoubtedly contains the samples that present a disordered structure. This cluster includes samples 1 to 10 (measured at denaturing conditions (i.e. at high ...
EXTENDED CONCEPT OF KNOWLEDGE FOR EVOLUTIONARY
EXTENDED CONCEPT OF KNOWLEDGE FOR EVOLUTIONARY

... makes it a gene, and what or who could be that 'somebody' (and in what sense). There is a real danger to take these metaphors too literally, and as a consequence to load them with spiritualist metaphysics or religious mysticism. I think this danger is one apparent reason why this kind of 'contentiou ...
Mitochondrial transfer: Ethical, legal and social implications in assisted reproduction
Mitochondrial transfer: Ethical, legal and social implications in assisted reproduction

... organs are severely affected, no preventative treatments are available and most patients experience a poor quality of life or early death. With developments in mitochondrial transfer techniques, hope for preventing transmission of mutated mtDNA onto offspring is emerging. Many ethical issues have be ...
1 X chromosome crossover formation and genome stability in
1 X chromosome crossover formation and genome stability in

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DNA polymerase active site is highly mutable
DNA polymerase active site is highly mutable

... The publication costs of this article were defrayed in part by page charge payment. This article must therefore be hereby marked “advertisement” in accordance with 18 U.S.C. §1734 solely to indicate this fact. ...
animal genetics
animal genetics

... All animals originate from the union of a single haploid cell from the female (ovum or egg) and a single haploid cell from the male (spermatozoa or sperm). The result of this union is a zygote (diploid cell), which develops into a new animal with a full set of chromosomes. Illustration of DNA Doubl ...
Power Point
Power Point

... All animals originate from the union of a single haploid cell from the female (ovum or egg) and a single haploid cell from the male (spermatozoa or sperm). The result of this union is a zygote (diploid cell), which develops into a new animal with a full set of chromosomes. Illustration of DNA Doubl ...
All research involving recombinant DNA molecules must comply
All research involving recombinant DNA molecules must comply

... 2. I will immediately report to the Biological Safety Officer any spill of biohazardous material, any equipment or facility failure (e.g., ventilation failure), and/or any breakdown in procedure that could result in potential exposure of laboratory personnel and/or the public to biohazardous materia ...
Geuvadis Analysis Meeting
Geuvadis Analysis Meeting

... - Quantified 615 datasets based on the Gencode v7 annotation - Sensitivity is a function of sequencing depth ...
Insights into Protein–DNA Interactions through Structure
Insights into Protein–DNA Interactions through Structure

... Protein–DNA interactions are crucial for many cellular processes. Now with the increased availability of structures of protein–DNA complexes, gaining deeper insights into the nature of protein–DNA interactions has become possible. Earlier, investigations have characterized the interface properties b ...
FREE Sample Here
FREE Sample Here

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Molecular events during translocation and proofreading extracted
Molecular events during translocation and proofreading extracted

... Figure 1. A cartoon representation of the main findings. DNA double helix is drawn as intertwined arcs as viewed along the major and minor grooves on one side of the DNA duplex. ␣ helices in the fingers domain are shown as cylinders. (A and C) viewed from a similar perspective show that the overall ...
letter Widespread aneuploidy revealed by DNA microarray expression profiling
letter Widespread aneuploidy revealed by DNA microarray expression profiling

... 4b,d) and contains RPL20B, which encodes a protein with 99% ecm18∆/ecm18∆ correlation, which was dependent on a large numidentity to Rpl20ap. It is tempting to speculate that the duplica- ber of small-magnitude expression changes arising from tion was a result of a homologous recombination event and ...
How is the biological information arranged in genome?
How is the biological information arranged in genome?

... 13,16-19], and an individual gene was an integral part of a genome. There were many genes and the associated regulatory regions that were expressed, replicated, transcribed and translated into proteins, and all participate in biological phenomena. Individual gene, i.e., a protein to be converted thr ...
structure and mechanism of dna polymerases
structure and mechanism of dna polymerases

... idea, proposed by Watson and Crick (1953a,b) based on the structure of DNA, was that selection could be determined by the A‐T and G‐C hydrogen bond–mediated base pairing. However, replicative polymerases have error rates for nucleotide insertion in the range of 10–3–10–6/base replicated and the diff ...
CRISPR: The Last Piece of the Genetic Puzzle
CRISPR: The Last Piece of the Genetic Puzzle

... What are CRISPR and Cas9? In 1987, researchers studying the bacteria E. coli noticed short repeated segments in the bacteria’s DNA. The repeated segments were grouped near each other on the bacteria’s chromosome separated by unique DNA sequences called spacers.2 This group of repeated segments and s ...
12–3 RNA and Protein Synthesis
12–3 RNA and Protein Synthesis

... Slide 24 of 39 Copyright Pearson Prentice Hall ...
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DNA supercoil



DNA supercoiling refers to the over- or under-winding of a DNA strand, and is an expression of the strain on that strand. Supercoiling is important in a number of biological processes, such as compacting DNA. Additionally, certain enzymes such as topoisomerases are able to change DNA topology to facilitate functions such as DNA replication or transcription. Mathematical expressions are used to describe supercoiling by comparing different coiled states to relaxed B-form DNA.As a general rule, the DNA of most organisms is negatively supercoiled.
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