Cloning in bacteria other than Escherichia coli
... Box 8.1 Transforming Bacillus subtilis with plasmid DNA Although it is very easy to transform B. subtilis with fragments of chromosomal DNA, there are problems associated with transformation by plasmid molecules. Ehrlich (1977) first reported that competent cultures of B. subtilis can be transformed ...
... Box 8.1 Transforming Bacillus subtilis with plasmid DNA Although it is very easy to transform B. subtilis with fragments of chromosomal DNA, there are problems associated with transformation by plasmid molecules. Ehrlich (1977) first reported that competent cultures of B. subtilis can be transformed ...
Investigation of a Zα-like Peptide Motif in Koi Herpesvirus
... stranded nucleic acids [14]. Significant amino acid sequence similarities are illustrated in Figure 1. This study suggests that ORF112 may contain an N-terminal Zα domain. It has been hypothesized that Z-DNA forms in vivo during transcription in alternating purinepyrimidine sequences. If the KHV pr ...
... stranded nucleic acids [14]. Significant amino acid sequence similarities are illustrated in Figure 1. This study suggests that ORF112 may contain an N-terminal Zα domain. It has been hypothesized that Z-DNA forms in vivo during transcription in alternating purinepyrimidine sequences. If the KHV pr ...
Applications of Genomics
... strands that are packaged by a large complex of supporting proteins into chromosomes. Humans have 23 pairs of chromosomes, including the pair that determines sex, which in women comprises 2 X chromosomes and in men 1 X and 1 Y chromosome (Figure 2). For each chromosome pair, 1 chromosome was inherit ...
... strands that are packaged by a large complex of supporting proteins into chromosomes. Humans have 23 pairs of chromosomes, including the pair that determines sex, which in women comprises 2 X chromosomes and in men 1 X and 1 Y chromosome (Figure 2). For each chromosome pair, 1 chromosome was inherit ...
Lecture Notes
... chromosome); monosomies of the autosomes are rare. B) Nondisjunction is the most common mechanism leading to aneuploidy. 1) Nondisjunction in mitosis at an early cleavage division may lead to a clinically significant mosaicism. ...
... chromosome); monosomies of the autosomes are rare. B) Nondisjunction is the most common mechanism leading to aneuploidy. 1) Nondisjunction in mitosis at an early cleavage division may lead to a clinically significant mosaicism. ...
Cytogenetics Cytogenetics
... and transferred to a new position in another chromosome, or rarely within the same chromosome. • Recorded as ins, followed by a bracket with the number of the chromosome which receives the segment preceding the number of the chromosome which donates the segment eg ins(2)(p13q31q34) and ins(5;2)(p12; ...
... and transferred to a new position in another chromosome, or rarely within the same chromosome. • Recorded as ins, followed by a bracket with the number of the chromosome which receives the segment preceding the number of the chromosome which donates the segment eg ins(2)(p13q31q34) and ins(5;2)(p12; ...
DNA Sequence Variation in the Human Y Chromosome: Functions
... specific (heteromorphic) sequences. We identified two such heteromorphic (male specific) sequences, though not involved in sex determination but showed cross hybridization with a few mammalian species [2, 6]. This indicates that some of the repeat sequences present on the human Y chromosome may have ...
... specific (heteromorphic) sequences. We identified two such heteromorphic (male specific) sequences, though not involved in sex determination but showed cross hybridization with a few mammalian species [2, 6]. This indicates that some of the repeat sequences present on the human Y chromosome may have ...
... 4. (14 pts) Draw the chemical structure of a tri-peptide, i.e. three amino acids linked together. The sidechain of the first amino acid is a methyl group (-CH3), the second is just a hydrogen atom, and the third is a isopropyl group (CH3-CH-CH3) (4 pts). i) Label a peptide bond in your drawing and i ...
Genes and RNA
... ribosomal RNA. The long filaments are DNA molecules coated with proteins. The fibers extending in clusters from the main axes are molecules of ribosomal RNA which will be used in the construction of the cell's ribosomes. Transcription begins at one end of each gene, with the RNA molecules getting lo ...
... ribosomal RNA. The long filaments are DNA molecules coated with proteins. The fibers extending in clusters from the main axes are molecules of ribosomal RNA which will be used in the construction of the cell's ribosomes. Transcription begins at one end of each gene, with the RNA molecules getting lo ...
... the hydrogen bond (1 pt) • Protein secondary structure - stabilized by hydrogen bonds. • DNA structure - hydrogen bonds stabilize the correct base pair. (3 pts for example) 2. (8 pts) In addition to hydrogen bonding, the following thermodynamic factors: i) van der Waals, ii) electrostatics, iii) hyd ...
Immobilization_Mecha..
... (30%), 11-mercaptoundecylphosphoric acid, 1-undecanethiol, ammonium hydroxide, 20× SSC (saline sodium citrate) buffer, poly-(α,β)DL-aspartic acid sodium salt, and poly-L-lysine hydrobromide were obtained from Sigma-Aldrich (St. Louis, MO). Hafnium dichloride oxide octahydrate was obtained from Alfa A ...
... (30%), 11-mercaptoundecylphosphoric acid, 1-undecanethiol, ammonium hydroxide, 20× SSC (saline sodium citrate) buffer, poly-(α,β)DL-aspartic acid sodium salt, and poly-L-lysine hydrobromide were obtained from Sigma-Aldrich (St. Louis, MO). Hafnium dichloride oxide octahydrate was obtained from Alfa A ...
Functional analysis of plastid DNA replication origins in tobacco by
... pea only two have been proposed due to the lack of an Inverted Repeat. oriA was localised within the rRNA operon, oriB downstream of the trnN gene. As the rRNA operon shows a high degree of conservation between different species, it appears possible that oriA is also located at this site in other sp ...
... pea only two have been proposed due to the lack of an Inverted Repeat. oriA was localised within the rRNA operon, oriB downstream of the trnN gene. As the rRNA operon shows a high degree of conservation between different species, it appears possible that oriA is also located at this site in other sp ...
PCR Reagents
... TaqNovaHS DNA Polymerase is a mixture of thermostable TaqNova DNA polymerase isolated from Thermus aquaticus and a highly specific monoclonal antibody, which acts as an inhibitor of the polymerization activity. The TaqNovaHS enables easy set up of a hot-start PCR reaction at room temperature. The an ...
... TaqNovaHS DNA Polymerase is a mixture of thermostable TaqNova DNA polymerase isolated from Thermus aquaticus and a highly specific monoclonal antibody, which acts as an inhibitor of the polymerization activity. The TaqNovaHS enables easy set up of a hot-start PCR reaction at room temperature. The an ...
Reconstruction of phylogenetic trees
... Models for DNA evolution (JC69) Proportion of site differences between two sequences in th JJukes-Canter the k C t model d l plotted l tt d against i t titime (# generations), starting from the common ancestor. ...
... Models for DNA evolution (JC69) Proportion of site differences between two sequences in th JJukes-Canter the k C t model d l plotted l tt d against i t titime (# generations), starting from the common ancestor. ...
Breaking PCR - Integrated DNA Technologies
... Overall, we have to conclude that the polymerase chain reaction is an even more forgiving technique than we first assumed. The nature of some of the robust results we obtained, particularly for MgCl2 concentration, primer annealing temperatures, and polymerase extension step duration, suggest that a ...
... Overall, we have to conclude that the polymerase chain reaction is an even more forgiving technique than we first assumed. The nature of some of the robust results we obtained, particularly for MgCl2 concentration, primer annealing temperatures, and polymerase extension step duration, suggest that a ...
CfE Higher Biology Unit 1: DNA and the Genome
... DNA can also be found within the chloroplasts of plant cells. It is usually larger than mitochondrial DNA and takes the form of circular chromosomes containing the genes involved in the photosynthetic process. Where circular DNA is found in eukaryotes, it is thought that it has been incorporated fro ...
... DNA can also be found within the chloroplasts of plant cells. It is usually larger than mitochondrial DNA and takes the form of circular chromosomes containing the genes involved in the photosynthetic process. Where circular DNA is found in eukaryotes, it is thought that it has been incorporated fro ...
64$ CfE Higher Biology Unit 1: DNA and the
... DNA can also be found within the chloroplasts of plant cells. It is usually larger than mitochondrial DNA and takes the form of circular chromosomes containing the genes involved in the photosynthetic process. Where circular DNA is found in eukaryotes, it is thought that it has been incorporated fro ...
... DNA can also be found within the chloroplasts of plant cells. It is usually larger than mitochondrial DNA and takes the form of circular chromosomes containing the genes involved in the photosynthetic process. Where circular DNA is found in eukaryotes, it is thought that it has been incorporated fro ...
64$ CfE Higher Biology Unit 1: DNA and the
... DNA can also be found within the chloroplasts of plant cells. It is usually larger than mitochondrial DNA and takes the form of circular chromosomes containing the genes involved in the photosynthetic process. Where circular DNA is found in eukaryotes, it is thought that it has been incorporated fro ...
... DNA can also be found within the chloroplasts of plant cells. It is usually larger than mitochondrial DNA and takes the form of circular chromosomes containing the genes involved in the photosynthetic process. Where circular DNA is found in eukaryotes, it is thought that it has been incorporated fro ...
Nucleosomal structure of sea urchin and starfish sperm chromatin
... histone H2B from sea urchin sperm could be reflected in chromatin structure we compared using micrococcal nuclease some parameters of sea urchin and starfish sperm chromatin. Starfish sperm cells have been chosen for such analysis since all the histones they contain, for exeption of histone H2B, see ...
... histone H2B from sea urchin sperm could be reflected in chromatin structure we compared using micrococcal nuclease some parameters of sea urchin and starfish sperm chromatin. Starfish sperm cells have been chosen for such analysis since all the histones they contain, for exeption of histone H2B, see ...
The nucleotide sequence of Saccharomyces cerevisiae
... Large-scale systematic sequencing has generally depended on the availability of an ordered library of large-insert bacterial or viral genomic clones for the organism under study. The generation of these large insert libraries, and the location of each clone on a genome map, is a laborious and time-c ...
... Large-scale systematic sequencing has generally depended on the availability of an ordered library of large-insert bacterial or viral genomic clones for the organism under study. The generation of these large insert libraries, and the location of each clone on a genome map, is a laborious and time-c ...
Assembly of additional heterochromatin distinct from centromere
... centromere assembly or centromere inactivation. CENP-A chromatin and centromere components, however, can be reassembled specifically on the ectopic alphoid YAC integration sites that correlate with transcriptional activation on the marker gene on the YAC sites (Nakano et al., 2003). The result indic ...
... centromere assembly or centromere inactivation. CENP-A chromatin and centromere components, however, can be reassembled specifically on the ectopic alphoid YAC integration sites that correlate with transcriptional activation on the marker gene on the YAC sites (Nakano et al., 2003). The result indic ...
DNA supercoil
DNA supercoiling refers to the over- or under-winding of a DNA strand, and is an expression of the strain on that strand. Supercoiling is important in a number of biological processes, such as compacting DNA. Additionally, certain enzymes such as topoisomerases are able to change DNA topology to facilitate functions such as DNA replication or transcription. Mathematical expressions are used to describe supercoiling by comparing different coiled states to relaxed B-form DNA.As a general rule, the DNA of most organisms is negatively supercoiled.