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Equality and Equity in Curriculum
Equality and Equity in Curriculum

... In 2014, NJ adopted the Next Generation Science Standards with the goal of ensuring our students graduate ready for college and career. The standards for science practice describe varieties of expertise that science educators at all levels should seek to develop in their students. These practices re ...
Microbial Diversity in Prince Edward County`s Soil Microbiome
Microbial Diversity in Prince Edward County`s Soil Microbiome

... water of the tube. The tube was swirled around and all cells were transferred to a different, labeled microbead tube. 50 microliters of solution MD1 was added to each microbead tube. All microbead tubes were heated to 65 degrees Celsius for 10 minutes and secured horizontally to a vortex, which was ...
SVD and PCA
SVD and PCA

... compute row (or column) sums compute sum of sums apply above formula to each entry of D Divide by –2 ...
Molecular Systematics of the Genus Zoogloea and Emendation of
Molecular Systematics of the Genus Zoogloea and Emendation of

... Phylogenetic relationships among strains of zoogloea and related taxa were determined by 16s rDNA sequencing and genomic DNA hybridizationtechniques. The 16s rRNA gene was amplified by the polymerase chain reaction with a pair of eubacterial consensus primers and sequenced directly by using an autom ...
video slide - SharpSchool
video slide - SharpSchool

... Working with the mold Neurospora crassa, George Beadle and Edward Tatum had isolated mutants requiring arginine in their growth medium and had shown genetically that these mutants fell into three classes, each defective in a different gene. From other considerations, they suspected that the metaboli ...
Human Heredity - Lyndhurst School
Human Heredity - Lyndhurst School

... Have students examine the photo and discuss the ways the people look different from one another and the ways they look similar. Help students connect the caption to the Big Idea of Information and Heredity by asking them to recall the role of DNA and its significance in heredity. (DNA carries the com ...
Nuclear Genes That Encode Mitochondrial Proteins
Nuclear Genes That Encode Mitochondrial Proteins

... Loci on Chromosome III in Arabidopsis An extensive survey of the Arabidopsis genome for genes that might be involved in mitochondrial genome maintenance functions revealed the presence of a number of genes on chromosome III that appeared to encode mitochondrial proteins based on their prokaryotic se ...
SVD and PCA COS 323
SVD and PCA COS 323

... compute row (or column) sums compute sum of sums apply above formula to each entry of D Divide by –2 ...
Lack of biological significance in the `linguistic features` of
Lack of biological significance in the `linguistic features` of

Construction of plant BAC libraries This document
Construction of plant BAC libraries This document

... 14. Add 30 µl of 0.5 M EDTA (pH 8.0) to each tube (this inhibits further enzyme activity) and gently agitate tubes to promote contact between the agarose and the EDTA. Keep tubes on ice. ...
Extension Activity 1: Plasmid Mapping STUDENT MANU AL
Extension Activity 1: Plasmid Mapping STUDENT MANU AL

... Scientists routinely take advantage of plasmid DNA and a natural bacterial defense mechanism, the restriction enzyme, as the basis for much of biotechnology. Restriction enzymes allow bacteria to destroy DNA from invading bacteriophages (phages) which are viruses that infect and destroy bacteria. Re ...
BI0I 121 cel]
BI0I 121 cel]

... Smallest of the RNA molecules; many different kinds. B. Single long strand that passes from the nucleus to the cytoplasm. C. Part of the mRNA that is translated into a polypeptide. D. Noncoding part of the mRNA transcript that is excised before the mRNA leaves the nucleus. E. Made in the nucleus; •p ...
light - Microbiology
light - Microbiology

... Stimulation of recombinant formation in Escherichia coli K 12 crosses by ultraviolet (u.v.) irradiation of the parental bacteria was first reported by Haas, Wyss & Stone (1948). Following the discovery of sexual differentiation, Hayes (1952) found that the effect is exclusively due to an increase in ...
Sex reversal: deletion mapping the male
Sex reversal: deletion mapping the male

... but otherwise phenotypically female individuals whose karyotype is 46,XY. They have "streak" ovaries devoid of follicles and no testicular tissue. The internal accessory structures are female. Secondary sexual characteristics are female but variably developed, and the somatic features of Turner synd ...
Next-Generation Sequencing applied to aDNA
Next-Generation Sequencing applied to aDNA

... sumatran_orangutan/ This folder contains trimmed reads (organized as in the makefile), and other intermediate files; these are often useful in other analyses, but may be deleted to save space. Next, genotype the coding genes on the mitochondrial genome and build a phylogeny using the following comma ...
Ultraviolet Induction of Chromosome Transfer by
Ultraviolet Induction of Chromosome Transfer by

... Stimulation of recombinant formation in Escherichia coli K 12 crosses by ultraviolet (u.v.) irradiation of the parental bacteria was first reported by Haas, Wyss & Stone (1948). Following the discovery of sexual differentiation, Hayes (1952) found that the effect is exclusively due to an increase in ...
Alisch RS, Wang T, Chopra P, Visootsak J, Conneely KN, Warren ST . Genome-wide analysis validates aberrant methylation in fragile X syndrome is specific to the FMR1 locus. BMC Med Genet. 2013 Jan 29;14:18. doi: 10.1186/1471-2350-14-18.
Alisch RS, Wang T, Chopra P, Visootsak J, Conneely KN, Warren ST . Genome-wide analysis validates aberrant methylation in fragile X syndrome is specific to the FMR1 locus. BMC Med Genet. 2013 Jan 29;14:18. doi: 10.1186/1471-2350-14-18.

... annotated the genome for all CGG trinucleotide repeats containing at least eight consecutive repeats (N = 136 tracts; N = 452 probes; see Methods) and found two juxtaposed probes annotated to ZFHX3 that reached significance (Additional file 6: Table S3). In contrast to the FMR1 locus, the ZFHX3 locu ...
Section D - Prokaryotic and Eukaryotic Chromosome Structure
Section D - Prokaryotic and Eukaryotic Chromosome Structure

... • Replication of phage λin vivo produces long linear molecules with multiple copies of the λ genome. These concatemers are ...
The Nterminal region of the bacterial DNA polymerase PolC features
The Nterminal region of the bacterial DNA polymerase PolC features

Using an Alu Insertion Polymorphism to Study Human
Using an Alu Insertion Polymorphism to Study Human

... is approximately 300 nucleotides in length. Alu owes its name to a recognition site for the endonuclease AluI in its middle. Although Alu is sometimes called a “jumping gene,” it is not properly a gene, because it does not produce a protein product. Alu transposons are found only in primate genomes ...
infection
infection

... Starnes lying in the hospital bed in front of her with her mother standing nearby. Picking up Kayla’s chart, Dr. Collins introduced herself to Kayla. “Hi, I’m Dr. Collins. I hear you’re not feeling very well.” Kayla replied weakly, “Hi ... am I gonna have surgery?” “I don’t know, Kayla. Let’s take a ...
PartFourAnswers.doc
PartFourAnswers.doc

... The trp operon is subject to regulation both by repression and by attenuation. Attenuation depends on the tight coupling between transcription and translation in bacteria. When the [Trp] is high, translation of the trp leader is completed and the ribosome blocks sequence 2. This allows the transcrib ...
The Co-Evolution of Genes and Culture Pedigrees
The Co-Evolution of Genes and Culture Pedigrees

... lactose tolerant. They can also be referred to as being lactase persistent, meaning that lactase production persists beyond childhood. (People who no longer produce lactase as adults are called lactase nonpersistent.) Genetic studies suggest that lactose tolerance arose among human populations in th ...
Human Biology - Genetics
Human Biology - Genetics

... of several experiments led scientists to agree that DNA was the substance responsible for the inheritance of traits. In this section you will learn about DNA. If DNA is responsible for the inheritance of traits, then what is a gene? We hear that word, and we read that word often. For example, “She i ...
Role of base-backbone and base-base interactions
Role of base-backbone and base-base interactions

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DNA supercoil



DNA supercoiling refers to the over- or under-winding of a DNA strand, and is an expression of the strain on that strand. Supercoiling is important in a number of biological processes, such as compacting DNA. Additionally, certain enzymes such as topoisomerases are able to change DNA topology to facilitate functions such as DNA replication or transcription. Mathematical expressions are used to describe supercoiling by comparing different coiled states to relaxed B-form DNA.As a general rule, the DNA of most organisms is negatively supercoiled.
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