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Virginia State Science Olympiad Regional Tournament
Virginia State Science Olympiad Regional Tournament

... c. Discontinuous ...
Bioinformatics Molecular Genetics
Bioinformatics Molecular Genetics

... Genes are the units of heredity in living organisms. Encoded in the organism's genetic material (usually DNA or RNA), & control the development and behavior of the organism. During reproduction, the genetic material is passed on from the parent(s) to the offspring. Genetic material can also be passe ...
A Study of the Asp110–Glu112 Region of EcoRII Restriction
A Study of the Asp110–Glu112 Region of EcoRII Restriction

Exporter la page en pdf
Exporter la page en pdf

... In higher eukaryotes, the absence of specific sequence motifs, marking the origins of replication has been a serious hindrance to the understanding of (i) the mechanisms that regulate the spatio-temporal replication program, and (ii) the links between origins activation, chromatin structure and trans ...
Chapter 6 – Exam style questions Q1. Bk Ch6 Exam MQ1 What is
Chapter 6 – Exam style questions Q1. Bk Ch6 Exam MQ1 What is

... that was needed for normal growth. They were able to establish that this resulted from the lack of a particular enzyme. They deduced which enzyme was missing in each case by exposing the different strains to a variety of nutrient combinations. After establishing that the mutations coincided with par ...
Document
Document

... advent of Southern transfer and the associated hybridization techniques made it possible for the first time to obtain information about the physical organization of single and multicopy sequences in complex genomes. The term Southern blotting, now used to describe any type of DNA transfer from gel t ...
Powerpoint Presentation
Powerpoint Presentation

... •The concentration of X-gal is constant at 0.5M within the cell at all times. •The concentration of X-gal is sufficiently large to have the rate of change of indigo be equal to Vmax. •Vmax is equal to Kcat multiplied by the concentration of β-galactosidase. •The concentration of β-galactosidase is e ...
DNA Polyacrylamide Gel Electrophoresis
DNA Polyacrylamide Gel Electrophoresis

IGEM_presentation
IGEM_presentation

... bi is the binding constant of component I to its activator, ei is the inverse rate of breakdown of component i. L relates to LexA, R to RecA, R* to RecA* and S to ssDNA Ki is the binding constant of component i to the LexA gene. Cs is the concentration of single stranded DNA ...
Introduction and Preliminaries - Department of Computer and
Introduction and Preliminaries - Department of Computer and

...  Made by genes (fragments of DNA) that are roughly three times longer than the corresponding proteins.  Why? Every 3 nucleotides in the DNA alphabet code one letter in the protein alphabet of amino ...
CHARACTERlZATION OF THE ~ 0 CHONDRIA . L DNA MOLECULE
CHARACTERlZATION OF THE ~ 0 CHONDRIA . L DNA MOLECULE

Unit 05 - Delivery guide
Unit 05 - Delivery guide

... The topic could be approached using a historical perspective, but is best approached in context. It is becoming increasingly clear that most human traits once thought of as demonstrating single gene inheritance, e.g. attached and detached ear lobes, are now known to involve more than one gene, so mo ...
Intelligent Icons: Integrating Lite-Weight Data Mining
Intelligent Icons: Integrating Lite-Weight Data Mining

... The three files in the example are ASCII text files, each of which contains approximately 16,000 base pairs of mitochondrial DNA. Here we used string edit distance as suggested in [15] to measure the distance between file names, and Euclidean distance to measure the distance between the file icons ( ...
Commonly Used STR Markers
Commonly Used STR Markers

What is Biotechnology
What is Biotechnology

... • An “RNA Club” organized by George Gamow (1954) assembled to determine the role of RNA in protein synthesis • Vernon Ingram’s research on sickle cell anemia (1956) tied together inheritable diseases with protein structure • Link made between amino acids and DNA ...
the association of chloroplast dna with photosynthetic membrane
the association of chloroplast dna with photosynthetic membrane

... vesicles free of central grana have not formed from this structure. The silver grains follow the relatively unswollen grana-rich regions, and not the swollen stromal regions. Most vesicles observed show the reported labelling patterns. Such patterns are not observed in the presence of the detergent ...
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Document

... • An “RNA Club” organized by George Gamow (1954) assembled to determine the role of RNA in protein synthesis • Vernon Ingram’s research on sickle cell anemia (1956) tied together inheritable diseases with protein structure • Link made between amino acids and DNA ...
An Introduction to Genetic Analysis Chapter 20 Transposable
An Introduction to Genetic Analysis Chapter 20 Transposable

... transposable genetic element, which is formally most correct and embraces the entire family of types. The term transposition has long been used in genetics to describe transfer of chromosomal segments from one position to another in major structural rearrangements. In the present context, what is be ...
MD Simulations of the P53 oncoprotein structure
MD Simulations of the P53 oncoprotein structure

Deconstructing the Genome: DNA at High Resolution
Deconstructing the Genome: DNA at High Resolution

... technology. The most important enzymes in this tool kit are ones that operate on the DNA molecule itself: restriction enzymes that cut DNA, ligases that join two molecules together, and polymerases that synthesize new DNA strands. The most important uncatalyzed biochemical reaction is hybridization: ...
13-1
13-1

... relationship between DNA and RNA. For example, a student might suggest a film negative for DNA and photographic prints of that negative for RNA. Then, have pairs or small groups of students discuss their analogies and choose a few that they think best model the roles of DNA and RNA. Ask students to s ...
Rosalind Elsie Franklin Physicist www.AssignmentPoint.com
Rosalind Elsie Franklin Physicist www.AssignmentPoint.com

... Crick and Watson then published their model in Nature on 25 April 1953 in an article describing the double-helical structure of DNA with only a footnote acknowledging "having been stimulated by a general knowledge of" Franklin and Wilkins' "unpublished" contribution. Actually, although it was the ba ...
Non-Type 1 Cystinuria Marker DNA Test for Mastiff
Non-Type 1 Cystinuria Marker DNA Test for Mastiff

... Before  explaining  our  DNA  findings,  let’s  review  some  basic  genetic  terminology.    All  mammals   (dogs,  people,  etc)  have  two  copies  of  every  chromosome,  except  the  sex  chromosomes,  where   females  have  two  cop ...
The whole paper can be downloaded here if you like.
The whole paper can be downloaded here if you like.

... the bacterial host to translate proteins from the plasmid to acquire antibiotic resistance and other characteristics. The second portion was called amplification and purification, it consisted of two parts as well. The process of amplification involves growing transformed bacteria in a bath of LB br ...
Replication of plasmids with the p15A origin in Shewanella
Replication of plasmids with the p15A origin in Shewanella

... this possibility, we similarly replaced the 383-bp DraI-NcoI fragment of the Cmr gene of pACYC184 with a 224-bp DraINcoI fragment of an MR-1 genomic ORF that complements a previously isolated mutant deficient in the reduction of Fe(III), nitrate and fumarate (Myers and Myers 1993b). After purificati ...
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DNA supercoil



DNA supercoiling refers to the over- or under-winding of a DNA strand, and is an expression of the strain on that strand. Supercoiling is important in a number of biological processes, such as compacting DNA. Additionally, certain enzymes such as topoisomerases are able to change DNA topology to facilitate functions such as DNA replication or transcription. Mathematical expressions are used to describe supercoiling by comparing different coiled states to relaxed B-form DNA.As a general rule, the DNA of most organisms is negatively supercoiled.
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