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广西医科大学理论课教案(1)
广西医科大学理论课教案(1)

... eye 想像 and believe that they could learn biochemistry very well ...
source file - MIMG — UCLA
source file - MIMG — UCLA

... Insert WebLogo of E. coli ribosome binding sites and start codons from http://molbiol-tools.ca/Motifs.htm ...
Nucleosides, Nucleotides,Nucleic Acids
Nucleosides, Nucleotides,Nucleic Acids

Nucleotide Sequence Preservation of Human
Nucleotide Sequence Preservation of Human

... sequence comparisons of the D-loop region of unrelated normal humans (5, 21). Three additional between-individual differences outside the Dloop region of mtDNA were identified in this study. Substitution of cytidine for thymidine at L-strand positions 9698 and 9725 was found in all clones containing ...
Spontaneous mutagenesis in exponentially growing and sta
Spontaneous mutagenesis in exponentially growing and sta

Foundations of Biology
Foundations of Biology

... left to right starting with the X chromosome distal left arm and ending with division 100 on the distal right arm of chromosome 3. Divisions are divided into lettered subdivisions going left to right ©2000 Timothy G. Standish ...
Chapter 21
Chapter 21

... The acentric fragment is lost during mitosis; this can be detected by the disappearance of dominant alleles in a heterozygote. Fusion between the broken ends of the chromosome generates dicentric chromosomes, which undergo further cycles of breakage and fusion. The fusion-breakage-bridge cycle is re ...
Gel Electrophoresis
Gel Electrophoresis

... of free radicals, polymerization is performed in closed cassettes. Sample application wells for vertical gels are formed at the upper edge of the gel during polymerization with the help of an inserted comb (see Figure 4). Sample wells for flatbed gels are made by using self-adhesive tape glued onto o ...
Chapter C4
Chapter C4

... 20 million.” The jury members were stunned by these figures. Can there be any doubt that the defendant is guilty? Continued on the next slide ...
The Fly Genome
The Fly Genome

... left to right starting with the X chromosome distal left arm and ending with division 100 on the distal right arm of chromosome 3. Divisions are divided into lettered subdivisions going left to right ©2000 Timothy G. Standish ...
Chapter 6
Chapter 6

... 20 million.” The jury members were stunned by these figures. Can there be any doubt that the defendant is guilty? Continued on the next slide ...
The coiled-coil of the human Rad50 DNA repair protein contains
The coiled-coil of the human Rad50 DNA repair protein contains

... polymer with a persistence length of 45 nm. For very small curvatures, the data closely matched the calculated energy curvature, validating the method. However, for curvatures exceeding 0.02 nm⫺1 the experimental data deviate substantially from the theoretical curve. Because of resolution limits of ...
Degenerate PCR - Yale School of Medicine
Degenerate PCR - Yale School of Medicine

... Inosine is a purine (which occurs naturally in tRNAs) that can form base pairs with cytidine, thymidine, and adenosine (although the inosine:adenosine pairing presumably doesn't fit quite correctly in double stranded DNA, so there may be an energetic penalty to pay when the helix bulges out at this ...
The agouti mouse model: an epigenetic
The agouti mouse model: an epigenetic

... The BPA study represents the first time the Avy model was employed to examine the effects of an environmental contaminant rather than a nutritional agent on the fetal epigenome. Unfortunately, current risk assessment policies have yet to incorporate environmental influences on the fetal epigenome in st ...
Homologous Recombination Between Episomal Plasmids and Chromosomes in Yeast.
Homologous Recombination Between Episomal Plasmids and Chromosomes in Yeast.

... mitotic recombination between closely linked markers. This result was reproduced in the mitotic crosses in Table 3, where factors of increase of more than tenfold relative to the spontaneous rate were found. However, the stimulation of recombination between plasmid and chromosome by the same doses o ...
ppt - eweb.furman.edu
ppt - eweb.furman.edu

... the carbonate minerals precipitate into microporous chimneys. High flux of carbon and energy is channeled over inorganic catalysts Thermal currents through pores can concentrate organics 1000 – 106 fold Vents persist for millennia ...
NOCARDIA sp. INDONESIAN VOLCANIC SOIL DESAK GEDE SRI ANDAYANI , ELIN YULINAH SUKANDAR
NOCARDIA sp. INDONESIAN VOLCANIC SOIL DESAK GEDE SRI ANDAYANI , ELIN YULINAH SUKANDAR

... Microorganisms that grow in extreme environments such as volcanic area have way of self-defense in order to survive its life. Nocardia sp. is one genus of this group which can find in volcanic soil will appear to be the most promising source of the future antibiotics. Ways in which microorganisms am ...
Encyclopedia of Nanotechnology
Encyclopedia of Nanotechnology

... biomolecular interactions in the living cell. DNA elasticity plays an important role in the interactions with proteins and protein complexes. It was demonstrated that by cooperative protein binding to DNA, proteins act as a powerful molecular machine [15]. The seminal experiments, which focused on p ...
Overexpression of the catalytic subunit of DNA polymerase results in
Overexpression of the catalytic subunit of DNA polymerase results in

20Sexual Reproduction, Meiosis, and Genetic Recombination
20Sexual Reproduction, Meiosis, and Genetic Recombination

... The hallmark of sexual reproduction is that genetic information contributed by two parents is brought together in a single individual. Because the offspring of sexual reproduction are diploid, the contribution from each parent must be haploid. The haploid cells produced by each parent that fuse toge ...
lac
lac

... determine whether it is transcribed or not. -Regulatory sequences are primarily ‘operators’ (repressor binding sequences). Can also include activator binding sequences. -Regulatory proteins work with regulatory sequences to control transcription of the operon. -Regulatory proteins act as activators ...
Chromosomes
Chromosomes

... fibers during mitosis and meiosis • Contains many repeats of a 171base sequence called the alpha satellite • Centromere associated proteins ▫ Some form kinetochore during ...
Background for the Recombinant DNA Lab
Background for the Recombinant DNA Lab

... Next, you will set up a PCR reaction designed to amplify the region of DNA surrounding the SNP (Fig. 1). We need to use PCR so we will have many copies of the particular DNA we are interested in; we do not want to try to experiment with the entire chromosome (remember how big it was!). We need many ...
Lab (3) Report: Population Genetics
Lab (3) Report: Population Genetics

Mitochondrial DNA and aging
Mitochondrial DNA and aging

... particular, in mtDNA, is ubiquitous, substantial and, like mortality rates, increases exponentially with age [35] It has been shown that exhalation of ethane and n-penthane, indicators of ROS-mediated lipid peroxidation increases with age [36]. mtDNA was shown to accumulate oxidative damage in an ag ...
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DNA supercoil



DNA supercoiling refers to the over- or under-winding of a DNA strand, and is an expression of the strain on that strand. Supercoiling is important in a number of biological processes, such as compacting DNA. Additionally, certain enzymes such as topoisomerases are able to change DNA topology to facilitate functions such as DNA replication or transcription. Mathematical expressions are used to describe supercoiling by comparing different coiled states to relaxed B-form DNA.As a general rule, the DNA of most organisms is negatively supercoiled.
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