Methods for detection of point mutations
... [1]. When low amounts of mutant alleles are analyzed in a large background of wild-type DNA, sensitivity can be increased by separation and detection of fluorescencelabeled fragments on a DNA sequencer [31]. Detectable mutations. In principle, all possible mutations are detectable by CCM [1, 30]. It ...
... [1]. When low amounts of mutant alleles are analyzed in a large background of wild-type DNA, sensitivity can be increased by separation and detection of fluorescencelabeled fragments on a DNA sequencer [31]. Detectable mutations. In principle, all possible mutations are detectable by CCM [1, 30]. It ...
Archives of Microbiology 167:
... from ligation of a 12-kb HpaI genomic fragment contained two plasmids; one appeared to be of the predicted 12-kb size, while the other was larger. Efforts to stabilize the desired plasmid by growth on either rich or minimal medium or by lowered temperature (30° C) were not successful. We were, howev ...
... from ligation of a 12-kb HpaI genomic fragment contained two plasmids; one appeared to be of the predicted 12-kb size, while the other was larger. Efforts to stabilize the desired plasmid by growth on either rich or minimal medium or by lowered temperature (30° C) were not successful. We were, howev ...
Updated ISSR and agarose gel protocol
... 1. Make gel solution (1.2% agarose solution) in a 250 mL flask. Mix together 1.08 g agarose and 90 mLs of 1X TBE buffer (in 5 gal. tub by sink in room with gel rigs) 2. Heat to boiling in microwave. I recommend reducing the microwave power to 50% to limit the loss of your gel from boilover. Watch th ...
... 1. Make gel solution (1.2% agarose solution) in a 250 mL flask. Mix together 1.08 g agarose and 90 mLs of 1X TBE buffer (in 5 gal. tub by sink in room with gel rigs) 2. Heat to boiling in microwave. I recommend reducing the microwave power to 50% to limit the loss of your gel from boilover. Watch th ...
KARNATAKA COMMON ENTRANCE TEST – MAY, 2016 BIOLOGY
... (1) a and c only (2) a and b only (3) b only (4) b and c only Ans (2) 49. Offsprings formed during sexual reproduction exhibits more variation than, those formed asexual method, because, (1) sexual reproduction is more complicated (2) genetic material comes from two different individuals (3) genetic ...
... (1) a and c only (2) a and b only (3) b only (4) b and c only Ans (2) 49. Offsprings formed during sexual reproduction exhibits more variation than, those formed asexual method, because, (1) sexual reproduction is more complicated (2) genetic material comes from two different individuals (3) genetic ...
Lab 7: Conjugation/Transformation
... Bacteria are haploid organisms that lack the "true nuclei" of eukaryotic organisms. Since they only have one copy of each gene, typically the genotypes of bacteria can be deduced easily from the phenotypes. Unlike the traits typically studied in eukaryotic organisms, bacteria have few distinct exter ...
... Bacteria are haploid organisms that lack the "true nuclei" of eukaryotic organisms. Since they only have one copy of each gene, typically the genotypes of bacteria can be deduced easily from the phenotypes. Unlike the traits typically studied in eukaryotic organisms, bacteria have few distinct exter ...
Adherin - Semantic Scholar
... the pre-replication complex that binds origins of DNA replication, reduced chromosomal association of the XScc2 adherin and cohesin. Moreover, depletion of the XOrc1 component of the origin recognition complex [6,19] or the Mcm2–7 complex that licenses origins [19], also blocked adherin binding. In ...
... the pre-replication complex that binds origins of DNA replication, reduced chromosomal association of the XScc2 adherin and cohesin. Moreover, depletion of the XOrc1 component of the origin recognition complex [6,19] or the Mcm2–7 complex that licenses origins [19], also blocked adherin binding. In ...
Identification
... cycle threshold (Ct) values less than 36 are considered as positive for P. ramorum, typically values are between 25-36. Ct values between 36-40 are rare and usually result from aerosol contamination these should be retested and the presence of P. ramorum confirmed by another means if the result is s ...
... cycle threshold (Ct) values less than 36 are considered as positive for P. ramorum, typically values are between 25-36. Ct values between 36-40 are rare and usually result from aerosol contamination these should be retested and the presence of P. ramorum confirmed by another means if the result is s ...
DNA phosphorothioation inStreptomyces lividans: mutational
... are dnd-specific and the Dnd proteins are correctly expressed in vivo. In the meantime, these expression plasmids carrying individual dnd genes could also rescue the Dnd phenotype of other corresponding mutants including HXY1 and HXY2 (data not shown). These complementation results from different mu ...
... are dnd-specific and the Dnd proteins are correctly expressed in vivo. In the meantime, these expression plasmids carrying individual dnd genes could also rescue the Dnd phenotype of other corresponding mutants including HXY1 and HXY2 (data not shown). These complementation results from different mu ...
Genit 3
... One homologous chromosome is inherited from the organism's mother; the other from the organism's father.[1] They are usually not identical. Each chromosome in the pair contains genes for the same biological features, such as eye color, at the same locations (loci) on the chromosome. However, each ca ...
... One homologous chromosome is inherited from the organism's mother; the other from the organism's father.[1] They are usually not identical. Each chromosome in the pair contains genes for the same biological features, such as eye color, at the same locations (loci) on the chromosome. However, each ca ...
K - Romanian Biotechnological Letters
... time interval and the ramping. Using a computer controlled device, only few parameters must be defined, every cycle consisting of a succession of steps (forward migration, pause, reverse migration, pause) being controlled by computer, according to the chosen ramp and parameters. It is obvious that, ...
... time interval and the ramping. Using a computer controlled device, only few parameters must be defined, every cycle consisting of a succession of steps (forward migration, pause, reverse migration, pause) being controlled by computer, according to the chosen ramp and parameters. It is obvious that, ...
1. Introduction
... the cell nucleus and previously described by Walther Flemming (1878) to be involved in the phenomenon of cell division (mitosis). During the first decade of the twentieth century, the Mendel’s laws of heredity in which each individual present two “factors” (identical or not) for each trait, with one ...
... the cell nucleus and previously described by Walther Flemming (1878) to be involved in the phenomenon of cell division (mitosis). During the first decade of the twentieth century, the Mendel’s laws of heredity in which each individual present two “factors” (identical or not) for each trait, with one ...
Chapter 18: Gene Mutation and DNA Repair
... affecting single genes. The purpose of this first section is to establish the basic types of mutations and the terminology associated with the study of mutations. There is a significant amount of descriptive terminology. Table 18.1 provides a very good overview. It is important that you have a good ...
... affecting single genes. The purpose of this first section is to establish the basic types of mutations and the terminology associated with the study of mutations. There is a significant amount of descriptive terminology. Table 18.1 provides a very good overview. It is important that you have a good ...
Highly precise and developmentally programmed genome
... thousands of short, non-coding germline sequences (Internal Eliminated Sequences or IESs), each one flanked by two TA dinucleotides. It has been reported previously that these genome rearrangements are initiated by the introduction of developmentally programmed DNA double-strand breaks (DSBs), which ...
... thousands of short, non-coding germline sequences (Internal Eliminated Sequences or IESs), each one flanked by two TA dinucleotides. It has been reported previously that these genome rearrangements are initiated by the introduction of developmentally programmed DNA double-strand breaks (DSBs), which ...
J Urol - prostata
... phosphatase and the amount of metastatic foci detected during radioisotope bone scan. Univariate survival analyses were performed and Cox's proportional hazards model was used to identify significant prognostic factors. To assess how the experimental factors improve the capacity of the classical fac ...
... phosphatase and the amount of metastatic foci detected during radioisotope bone scan. Univariate survival analyses were performed and Cox's proportional hazards model was used to identify significant prognostic factors. To assess how the experimental factors improve the capacity of the classical fac ...
Phylogenetic Affinity of Mitochondria of Euglena
... RNA molecules called guide RNAs mediate the uridine insertion/deletion type of RNA editing (Simpson et al. 1993). It is known that these guide RNA molecules can be capped in vitro with guanylyl transferase and GTP (Blum and Simpson 1990). To search for similar RNA species in E. gracilis mitochondria ...
... RNA molecules called guide RNAs mediate the uridine insertion/deletion type of RNA editing (Simpson et al. 1993). It is known that these guide RNA molecules can be capped in vitro with guanylyl transferase and GTP (Blum and Simpson 1990). To search for similar RNA species in E. gracilis mitochondria ...
DNA supercoil
DNA supercoiling refers to the over- or under-winding of a DNA strand, and is an expression of the strain on that strand. Supercoiling is important in a number of biological processes, such as compacting DNA. Additionally, certain enzymes such as topoisomerases are able to change DNA topology to facilitate functions such as DNA replication or transcription. Mathematical expressions are used to describe supercoiling by comparing different coiled states to relaxed B-form DNA.As a general rule, the DNA of most organisms is negatively supercoiled.