Towards Programmable Molecular Machines
... Tian et al. [10] also proposed a walker using DNAzymes. In this construction, the walker is a DNAzyme. It attaches to single stranded DNAs anchored on the substrate, makes a cut and moves onto the next DNA strand. Pei et al. [4] extended the DNAzyme walker to a 2-dimensional walker. In their design, ...
... Tian et al. [10] also proposed a walker using DNAzymes. In this construction, the walker is a DNAzyme. It attaches to single stranded DNAs anchored on the substrate, makes a cut and moves onto the next DNA strand. Pei et al. [4] extended the DNAzyme walker to a 2-dimensional walker. In their design, ...
Translocation Breakpoints Are Clustered on Both Chromosome 8
... within it can be investigated using probes for the two fragments generated by BamHI digestion. Thus, two probes were prepared (Fig 1): the first (21A)detects the telomeric BamHI fragment (normal size of I 1 kbp), is 500 bp long and spans the whole of the fifth exon, extending to the Sac1 site immedi ...
... within it can be investigated using probes for the two fragments generated by BamHI digestion. Thus, two probes were prepared (Fig 1): the first (21A)detects the telomeric BamHI fragment (normal size of I 1 kbp), is 500 bp long and spans the whole of the fifth exon, extending to the Sac1 site immedi ...
Molecular characterization of dioxygenases from polycyclic aromatic
... results (Table 1). Fig. 3 shows the comparison between Southern blot patterns of XbaI-digested DNA blotted with the nidA and nidB gene probes respectively (negative controls listed in Table 1). One band from Mycobacterium sp. PAH 2.135 (RJGII-135) hybridized at 48 kb with both nidA and nidB probes ( ...
... results (Table 1). Fig. 3 shows the comparison between Southern blot patterns of XbaI-digested DNA blotted with the nidA and nidB gene probes respectively (negative controls listed in Table 1). One band from Mycobacterium sp. PAH 2.135 (RJGII-135) hybridized at 48 kb with both nidA and nidB probes ( ...
Mycobacterium tuberculosis DNA gyrase ATPase domain structures
... [7,8] and ATPase activity [9,10]. The structure of the ATPase domain of the GyrB subunit of Escherichia coli DNA gyrase [7] showed that it contained two structural domains, an N-terminal GHKL domain [11] and a Cterminal transducer domain (Figure 1). A change in the relative positions of the GHKL and ...
... [7,8] and ATPase activity [9,10]. The structure of the ATPase domain of the GyrB subunit of Escherichia coli DNA gyrase [7] showed that it contained two structural domains, an N-terminal GHKL domain [11] and a Cterminal transducer domain (Figure 1). A change in the relative positions of the GHKL and ...
Grade 12 - Curriculum
... Lesson Plans are working documents, and any comments to improve the lesson plans in this document will be appreciated. Teachers are urged to use this document with the following departmental policy documents: Subject Statement; LPG 2008; SAG 2008; Examination Guidelines 2009 and Provincial CASS Poli ...
... Lesson Plans are working documents, and any comments to improve the lesson plans in this document will be appreciated. Teachers are urged to use this document with the following departmental policy documents: Subject Statement; LPG 2008; SAG 2008; Examination Guidelines 2009 and Provincial CASS Poli ...
UV-Targeted Dinucleotides Are Not Depleted in Light
... genomes and its link with environmental or metabolic characteristics has been the focus of considerable interest. It has been established that ultraviolet (UV) light—at both UVb (290–320 nm) and UVa wavelengths (320– 400 nm)—damages DNA by specific mechanisms. It has been shown that UVb wavelengths ...
... genomes and its link with environmental or metabolic characteristics has been the focus of considerable interest. It has been established that ultraviolet (UV) light—at both UVb (290–320 nm) and UVa wavelengths (320– 400 nm)—damages DNA by specific mechanisms. It has been shown that UVb wavelengths ...
Microbiology
... Two transposon Tn5-induced mutants of wild-type broad-host-range Rhizobium sp. GRHZ were isolated and found to harbour different alterations in surface polysaccharides. These mutants, designated GRHZ-14 and GRHZ-50, induced a few, empty nodules on Acacia and lost the ability to nodulate most host he ...
... Two transposon Tn5-induced mutants of wild-type broad-host-range Rhizobium sp. GRHZ were isolated and found to harbour different alterations in surface polysaccharides. These mutants, designated GRHZ-14 and GRHZ-50, induced a few, empty nodules on Acacia and lost the ability to nodulate most host he ...
Surface polysaccharide mutants of Rhizobium sp. (Acacia) strain
... Two transposon Tn5-induced mutants of wild-type broad-host-range Rhizobium sp. GRHZ were isolated and found to harbour different alterations in surface polysaccharides. These mutants, designated GRHZ-14 and GRHZ-50, induced a few, empty nodules on Acacia and lost the ability to nodulate most host he ...
... Two transposon Tn5-induced mutants of wild-type broad-host-range Rhizobium sp. GRHZ were isolated and found to harbour different alterations in surface polysaccharides. These mutants, designated GRHZ-14 and GRHZ-50, induced a few, empty nodules on Acacia and lost the ability to nodulate most host he ...
What is Cytogenetics?
... specific DNA sequences on chromosomes. • FISH uses fluorescent probes which bind only to those parts of the chromosome with which they show a high degree of sequence similarity. • Generally between 200-1000 cells are counted. ...
... specific DNA sequences on chromosomes. • FISH uses fluorescent probes which bind only to those parts of the chromosome with which they show a high degree of sequence similarity. • Generally between 200-1000 cells are counted. ...
Applied and Environmental Microbiology
... volume of 20 l at 37°C overnight. After the addition of 0.5 l ...
... volume of 20 l at 37°C overnight. After the addition of 0.5 l ...
Plant centromeres: structure and control Eric J Richards and R Kelly
... itive DNA [18]. It is likely that this block corresponds, on the cytological level, to the heterochromatin that encompasses the region associated with the kinetochore. This pericentric heterochromatin appears to have a role in controlling and/or mediating sister chromatid cohesion in metaphase (of m ...
... itive DNA [18]. It is likely that this block corresponds, on the cytological level, to the heterochromatin that encompasses the region associated with the kinetochore. This pericentric heterochromatin appears to have a role in controlling and/or mediating sister chromatid cohesion in metaphase (of m ...
Catellibacterium aquatile sp. nov., isolated from fresh water, and
... fragment was determined by direct sequencing and compared with available 16S rRNA gene sequences in GenBank using the BLAST program (Altschul et al., 1990). Multiple alignment with closely related sequences was performed by using the CLUSTAL_X program (Thompson et al., 1994). Ambiguous and unalignab ...
... fragment was determined by direct sequencing and compared with available 16S rRNA gene sequences in GenBank using the BLAST program (Altschul et al., 1990). Multiple alignment with closely related sequences was performed by using the CLUSTAL_X program (Thompson et al., 1994). Ambiguous and unalignab ...
Genomic and Physiological Comparisons Between Heterotrophic
... insight is supported by the homology results shown in Table 1. If the two organisms differ by one or a few mutations, their DNAs should show nearly 100% homology (within the experimental precision of the method used, which is -5 to 8% homology). However, data in Table 1 do indicate a close relations ...
... insight is supported by the homology results shown in Table 1. If the two organisms differ by one or a few mutations, their DNAs should show nearly 100% homology (within the experimental precision of the method used, which is -5 to 8% homology). However, data in Table 1 do indicate a close relations ...
Aberrant replication timing induces defective chromosome
... homologous subunits or even whole complexes are functionally interchangeable between species, suggesting a high degree of conservation of ORC function [3–5]. The Drosophila ORC complex was isolated from embryonic extracts by functional homology to the Saccharomyces cerevisiae complex, and all six Dr ...
... homologous subunits or even whole complexes are functionally interchangeable between species, suggesting a high degree of conservation of ORC function [3–5]. The Drosophila ORC complex was isolated from embryonic extracts by functional homology to the Saccharomyces cerevisiae complex, and all six Dr ...
Genomic and Physiological Comparisons Between Heterotrophic
... insight is supported by the homology results shown in Table 1. If the two organisms differ by one or a few mutations, their DNAs should show nearly 100% homology (within the experimental precision of the method used, which is -5 to 8% homology). However, data in Table 1 do indicate a close relations ...
... insight is supported by the homology results shown in Table 1. If the two organisms differ by one or a few mutations, their DNAs should show nearly 100% homology (within the experimental precision of the method used, which is -5 to 8% homology). However, data in Table 1 do indicate a close relations ...
Comparative Genomics II.
... • The one of β genes is a pseudogene that has a mutation which prevents its expression. The sequences of these six genes are quite similar which suggests they occurred by duplication of an ancestral β-globin gene • Once a gene duplication event has occurred evolutionary constraints working upon thes ...
... • The one of β genes is a pseudogene that has a mutation which prevents its expression. The sequences of these six genes are quite similar which suggests they occurred by duplication of an ancestral β-globin gene • Once a gene duplication event has occurred evolutionary constraints working upon thes ...
Gene as the unit of genetic material - E
... Autopolyploid - an individual that has an additional set of chromosomes that are identical to parental species; an autotriploid would have the chromosomal composition of AAA and an autotetraploid would be AAAA; both of these are in comparison to the diploid with the chromosomal composition of AA All ...
... Autopolyploid - an individual that has an additional set of chromosomes that are identical to parental species; an autotriploid would have the chromosomal composition of AAA and an autotetraploid would be AAAA; both of these are in comparison to the diploid with the chromosomal composition of AA All ...
DNA supercoil
DNA supercoiling refers to the over- or under-winding of a DNA strand, and is an expression of the strain on that strand. Supercoiling is important in a number of biological processes, such as compacting DNA. Additionally, certain enzymes such as topoisomerases are able to change DNA topology to facilitate functions such as DNA replication or transcription. Mathematical expressions are used to describe supercoiling by comparing different coiled states to relaxed B-form DNA.As a general rule, the DNA of most organisms is negatively supercoiled.